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Brief Introduction

English Alias：Astragaloside IV; beta-D-Glucopyranoside, (3beta,6alpha,16beta,24R)-20,24-epoxy-16,25-dihydroxy-3-(beta-D-xylopyranosyloxy)-9,19-cyclolanostan-6-yl; (3beta,6alpha,9beta,16beta,20R,24S)-16,25-dihydroxy-3-(beta-D-xylopyranosyloxy)-20,24-epoxy-9,19-cyclolanostan-6-yl beta-D-threo-hexopyranoside

Molecular Formula：C41H68O14

Chemical Name: 17-[5-(1-Hydroxyl-1-methyl-ethyl)- 2methyl-tetrahydro- furan-2-yl]-4,4,13,14-tetramethyl-tetradecahydro-cyclopropa[9,10]cyclopenta[a]phenanthren-16-ol-3-β-D-aracopyranosyl-6-β-D- glucoside

Mp: 200~204℃

[α]D:-56.6（c,0.13 in DMF）

UV: λmax203 nm

purity：98%

Source: legume Astragalus membranaceus, Astragalus pubescens.

Chemical structure formula of astragaloside IV

Physicochemical Properties

[appearance]: white crystalline powder

[purity]: above 98%, detection method: HPLC

[plant source]: the roots of Astragalus Alexandrinus Boiss, Astragalus dissectus, Astragalus membranaceus (Fisch.) Bungede root, Astragalus sieversianus Pall Root of Astragalus spinosus Vahl, aerial part of Astragalus spinosus Vahl.

[product properties]: Astragalus membranaceus extract is brown yellow powder.

[content determination]: determine by HPLC (Appendix VI D, Volume I, Chinese Pharmacopoeia, 2010 Edition).

Chromatographic conditions and system applicability test} octadecyl silane bonded silica gel is used as filler, acetonitrile water (32:68) is used as mobile phase, and evaporative light scattering detector is used for detection. The number of theoretical plates shall not be less than 4000 according to astragaloside IV peak.

Preparation of reference solution, take an appropriate amount of astragaloside IV reference, accurately weigh it, and add methanol to prepare a solution containing 0.5mg per 1ml.

Preparation of test solution: take about 4G of powder from this product, weigh it accurately, put it in Soxhlet extractor, add 40ml of methanol, soak it overnight, add an appropriate amount of methanol, heat and reflux for 4 hours, recover the solvent from the extract and concentrate it to dry, add 10ml of water to dissolve the residue, shake and extract it with saturated n-butanol for 4 times, 40ml each time, combine n-butanol solution, and wash it fully with ammonia test solution for 2 times, 40ml each time, discard the ammonia solution, evaporate the n-butanol solution, add 5ml water to dissolve the residue, and cool it, Through D101 macroporous adsorption resin column (inner diameter: 37.5px, column height: 300px), elute with 50ml of water, discard the water solution, elute with 30ml of 40% ethanol, discard the eluent, elute with 80ml of 70% ethanol, collect the eluent, evaporate it to dryness, dissolve the residue with methanol, transfer it to a 5ml volumetric flask, add methanol to the scale, shake well, and then get it.

Determination method: accurately absorb 10% of the reference solution respectively μ l、20 μ l. Test solution 20 each μ l. Inject it into the liquid chromatograph, determine it, and calculate it with the logarithm equation of the external standard two-point method.

Calculated as a dry product, the content of astragaloside IV (c41h68o14) shall not be less than 0.040%

Pharmacological Action

The main effective components of Astragalus are polysaccharides and astragaloside. Astragaloside is divided into astragaloside I, astragaloside II and astragaloside IV. Among them, astragaloside IV, astragaloside IV, has the best biological activity. Astragaloside IV not only has the effect of Astragalus polysaccharides, but also some incomparable effects of Astragalus polysaccharides. Its efficacy intensity is more than twice that of conventional astragalus polysaccharides, and its antiviral effect is 30 times that of Astragalus polysaccharides. Because of its low content and good effect, it is also known as “super astragalus polysaccharide”.

1.Enhance immunity and disease resistance.
It can specifically and nonspecifically exclude foreign bodies invading the body, promote specific, immune and nonspecific immunity, and improve the disease resistance of the body. It can promote the production of antibody, and significantly enhance the number of antibody forming cells and hemolysis test value. Astragaloside IV can significantly improve the lymphocyte transformation level and E-rosette formation rate of coccidia immunized chickens. It is an effective activator of monocyte macrophage system. Astragaloside IV can also improve oxidation, GSH-Px and SOD activities in immune organs, and improve immune defense and immune monitoring functions.

2.Antiviral effect.
Its antiviral principle: stimulate the function of macrophages and T cells, increase the number of E-ring forming cells, induce cytokines, promote the induction of interleukin, and make the animal body produce endogenous interferon, so as to achieve the purpose of antiviral. The results showed that the total protective rate of astragaloside IV on IBD was 98.33%, which could effectively prevent and treat IBD, and there was no significant difference compared with high immune egg yolk solution. Astragaloside can enhance the function of antioxidant enzymes in the body, reduce the content of LP0, reduce the damage of reactive oxygen species, and thus reduce the incidence rate and mortality of MD. It can improve the low immune function caused by tumor, promote the activation of immune cells, release endogenous factors, and prevent the killing and inhibition of tumor cells caused by peroxidation; Astragaloside a can inhibit the growth of influenza virus and the activity of sialidase. It has a significant effect on the function of influenza virus cell membrane and the adsorption and penetration of virus to sensitive cells. The mortality and egg laying rate of poultry were greatly reduced, and the egg laying rate and eggshell quality recovery were significantly better than those of amantadine only control group, and the effect of Astragalus polysaccharide was not obvious; Astragaloside IV has strong killing and inhibitory effects on nd virus. The premise is that the use of astragaloside IV is prior to the discovery of infection with Nd virus, so it is best to use astragaloside IV for a long time, Avian myeloblastic leukemia (AMB) 3 day old AA broilers fed astragaloside IV with the infection of AMB virus, could reduce the incidence rate and mortality of AMB, increase the LPO content in immune organs such as spleen and thymus, significantly enhance the scavenging effect of spleen and thymus and other immune organs on myeloid derived tumor cells. Secondly, astragaloside IV has obvious preventive and therapeutic effects on respiratory diseases such as infectious laryngotracheitis. Use.

3. Anti stress effect.
Astragaloside IV can prevent adrenal hyperplasia and thymus atrophy in the warning period of stress response, and prevent the abnormal changes in the resistance period and failure period of stress response, so as to play an anti stress role, especially has a significant two-way regulation effect on enzymes in the process of nutrient metabolism, and reduces and eliminates the impact of heat stress on the physiological function of the body to a certain extent.

4. As a growth promoter.
It can enhance the physiological metabolism of cells, promote blood circulation, enhance the metabolism of animal body, and play the role of nutrition and health care. Research shows that it can promote the growth of bifidobacteria and lactic acid bacteria and has the effect of probiotics.

5. Astragaloside IV can improve cardiopulmonary function.
Strengthen cardiac contractility, protect myocardium and resist heart failure. It also has the effects of protecting liver, anti-inflammatory and analgesic. It can be used as an adjuvant therapy for various viral and bacterial diseases.