Liquiritin, Glycyrrhizin;Liquiritoside;Likvirtin;Liquiritoside Cas No.551-15-5
Takaitaccen Gabatarwa
Glycyrrhizin, kuma aka sani da Liquiritin.Licorice shuka ne na Glycyrrhiza a cikin Leguminosae.Tushensa da mai tushe ganye ne na Sinawa na kowa.
Ana rarraba magunguna sosai a arewa maso gabashin kasar Sin, Xinjiang, Yunnan, Mongoliya ta ciki, Anhui da sauran wurare.Shennong materia medica classic ya jera shi a matsayin babban matsayi, yana mai cewa "wannan ciyawa ita ce sarkin duk magunguna, kuma akwai 'yan kaɗan waɗanda ba sa amfani da ita".Licorice yana da hadaddun abubuwan da suka hada da triterpenoids, flavonoids da coumarins.Flavonoids wani nau'i ne na abubuwan da ake amfani da su na bioactive da aka samo daga cirewar licorice.Abubuwan da ke cikin sinadarai na magani sun hada da glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, da dai sauransu. A cikin 'yan shekarun nan, an sami rahotanni game da sauye-sauyen radical, antioxidant, anti-cancer da anti mutagenic sakamakon licorice flavonoids a gida da waje.
ChemicalName:4H-1-Benzopyran-4-daya, 2- [4- (β-D-glucopyranosyloxy) phenyl] -2, 3-dihydro-7-hydroxy-, (S)
PhysicalPdukiya:Monohydrate (dilute ethanol ko ruwa), wurin narkewa: 212 ~ 213 ° ℃.
Ayyukan Pharmacological
Guba: Babu
Mummunan halayen: ba a sani ba
Tushen tushen: legume Glycyrrhiza glabra L. tushen, Glycyrrhiza uralensis Fisch Tushen.
Ana cire Glycyrrhizin
Pretreatment Na Licorice Raw Materials
Abubuwan sinadaran na kayan albarkatun licorice yana da matukar rikitarwa.Don samun sakamako mafi kyau na rabuwa, rage gurɓataccen ƙazanta a kan ginshiƙi na shirye-shiryen chromatographic, da haɓaka abun ciki na glycyrrhizin a cikin albarkatun ƙasa na allura, an yi amfani da hanyar cirewa don pretreat da albarkatun ƙasa.Auna hay 4G tare da ma'auni na lantarki kuma saka shi a cikin beaker.A auna ruwa mai tsafta 100ml daidai tare da silinda mai aunawa a zuba a cikin baƙar don narkewa.Ultrasonic na kimanin minti 15, kuma yana motsawa kullum tare da sandar gilashi don hanzarta rushewa.Sa'an nan kuma sanya beaker a cikin wanka mai zafi na 90 ℃ a zafi shi tsawon awanni 2, sannan a zafi shi don tacewa.Bayan an ƙara tacewa a cikin n-butanol solvent kuma a tsaye na tsawon mintuna da yawa, yawancin glycosides suna narkar da su a cikin n-butanol solvent, sannan a gudanar da cirewa na biyu, a fitar da ƙaramin adadin glycosides da suka rage a cikin ruwa, sannan a haɗa su da mayar da hankali ga n- maganin butanol da aka samu ta hanyar cirewa na biyu don chromatography da tsarkakewa.
Tsarkake Glycyrrhizin Ta Chromatography
Ɗauki 10 ml na samfurin da aka samo a sama a matsayin kayan da aka keɓe, fara famfo, saita ƙimar gudana a 25 ml / min, kuma kawo albarkatun cikin 500 mm ta hanyar wayar hannu (methanol: ruwa = 1: 4) × A cikin 40 mm ginshiƙi shirye-shirye, tattara gungu na hay glucoside samfurin bisa ga kololuwar halin da ake ciki: da kashi na farko 1 h aka tattara tare a matsayin pre-jasa juzu'i, sa'an nan canza kwarara.Misali, wanke ginshiƙi tare da cakuda methanol 50% da ruwa, haɗa samfurin kowane minti 20, sannan a tattara kowane kwalban samfurin tare da ƙanƙara mai jujjuyawa, sannan ɗauki 20 µ L don nazarin chromatographic na HPLC, Har sai ba a gano manufa ba.Sharuɗɗan gano HPLC sune kamar haka: lokacin wayar hannu: methanol: ruwa = 3.5: 6.5;Tsawon lokaci: silica gel carbon 18;Shafin Chromatographic: 450 mm × 4.6 mm; Yawan gudana: 1 ml / min;Tsawon Gano: 254nm.Abubuwan da ke cikin glycyrrhizin a cikin kwalba na biyu shine mafi girma a cikin samfuran da aka karɓa kowane minti 20
Tsarkake Glycyrrhizin Ta Rechromatography
Tun da abun ciki na glycyrrhizin bayan tsarkakewar chromatographic na farko ba shi da yawa, ana zaɓi hanyar iri ɗaya.Ɗauki 10 ml na samfurin da aka tsarkake a sama a matsayin kayan aiki na jiran aiki, ƙimar kwarara shine 25 ml / min, kuma kawo kwalban samfurin na biyu zuwa 500 mm ta hanyar wayar hannu (methanol: ruwa = 2: 5) × A cikin 20 mm } chromatographic shafi, tattara distillate na hay glycoside samfurin bisa ga kololuwar halin da ake ciki: haɗa samfurin kowane 4 min, sa'an nan kuma mayar da hankali kowane kwalban samfurin tare da Rotary evaporation, da kuma amfani da wannan ganewa tsiri a sama don HPLC chromatographic bincike har sai da babu manufa. .Bayan bincike, an gano cewa abun ciki na glycyrrhizin a cikin kwalban na shida shine mafi girma a cikin samfuran da aka karɓa kowane minti 4, wanda lokacin riƙewa ya kasance 5.898 min a matsayin mafi girman manufa, kuma abun ciki ya kai kusan 40% ta hanyar daidaita yanayin yanki. .
Post Jiyya Na Products
Samfurin da aka tattara yana distilled a ƙarƙashin rage matsa lamba akan injin juyawa a 70 ℃.Bayan da sauran ƙarfi ya fito, sai a narkar da ƙaƙƙarfan samfurin a kan kwandon ƙasa zagaye da ɗan ƙaramin methanol, sannan a yi crystallize a cikin bututun gwaji a yanayin ɗaki har sai fararen lu'ulu'u ya bayyana [2].
