Kayayyaki

Bayani:isoorientin shine ingantacciyar COX-2 mai hanawa tare da ƙimar IC50 na 39 μM.

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manufa: cox-2:39 μM (IC50)

Nazarin in vitro:Isoorientin wani zaɓi ne mai hanawa na cyclooxygenase-2 (COX-2) daga tuber na Pueraria tuberosa [1].Kwayoyin PANC-1 da patu-8988 an bi da su tare da Isoorientin (0,20,40,80 da 160 μ M) Girma a gaban 24 hours kuma ƙara CCK8 bayani.A 20, 40, 80 da 160 μ A ƙaddamarwa na M, haɓakar kwayar halitta ya ragu sosai. An yi amfani da Isoorientin (0,20,40,80 da 160) don sel μ M don PANC-1;0, 20, 40, 80160 da 320 μM an yi amfani da su don patu-8988) al'ada na sa'o'i 24, kuma an tantance furcin P ta Western blot - AMPK da AMPK.Maganar p-ampk ya karu bayan jiyya na Isoorientin.Sa'an nan, a cikin rukunin shRNA, 80 μM maida hankali don gano tasirin Isoorientin.Matakan magana na AMPK da p-ampk a cikin rukunin shRNA sun kasance ƙasa da waɗanda ke cikin nau'ikan PC na daji (WT) kuma ƙungiyar ta canza tare da lentivirus mara kyau (NC) [2].

A cikin nazarin vivo:Dabbobin da aka yi amfani da su tare da Isoorientin a 10 mg / kg da nauyin jiki na 20 mg / kg sun sami raguwar ƙididdiga mai mahimmanci a cikin edema, tare da matsakaicin kauri na 1.19 ± 0.05 mm da 1.08 ± 0.04 mm, bi da bi.Wannan ya nuna cewa Isoorientin ya rage girman edema idan aka kwatanta da ƙungiyar kulawa [3].

Gwajin kwayar halitta:An yi allurar sel PANC-1 da patu-8988 akan faranti 96 rijiya.Kowace rijiya ta ƙunshi sel ~ 5000 da sel 200 μ L matsakaici mai ɗauke da 10% FBS.Lokacin da sel a cikin kowace rijiya suka kai kashi 70%, an canza matsakaici kuma an ƙara matsakaicin kyauta na FBS tare da nau'ikan isoorientin daban-daban.Bayan sa'o'i 24, an wanke sel sau ɗaya tare da PBS, an watsar da matsakaicin al'adun da ke ɗauke da isoorientin, kuma an ƙara 100% μ L FBS matsakaici kyauta da 10 μ L cell kit 8 (CCK8) reagent.An shigar da ƙwayoyin sel a 37 ℃ na wasu sa'o'i 1-2, kuma an gano shayar da kowace rijiya a 490 nm ta amfani da mai karanta ELISA.Ana bayyana iyawar tantanin halitta azaman canji mai yawa a cikin abin sha [2].

Gwajin dabba:Dangane da samfurin paw edema, an ba mice [3] isoorientin ko celecoxib intraperitoneally, kuma an yi wa carrageenan allurar kai tsaye a cikin paw bayan sa'a ɗaya.A cikin samfurin airbag, duk jiyya sun shiga ramin jakar kai tsaye tare da carrageenan.An yi allurar isoorientin sa'o'i 3 kafin a yi wa carrageenan allurar a cikin capsule.An ba da Isoorientin da celecoxib ga beraye.Maganin jari na isoorientin (100 mg / ml) da celecoxib (100 mg / ml) an shirya su a cikin DMSO kuma an ƙara diluted yayin jiyya.An raba dabbobi zuwa kungiyoyi daban-daban guda biyar masu zuwa: sarrafawa (DMSO da aka bi da su);Maganin carrageenan (0.5 ml (1.5% (w / V) carrageenan a cikin brine); Maganin carrageenan + celecoxib (20mg / kg nauyin jiki); Maganin carrageenan + isoorientin (10 mg / kg nauyin jiki); kg jiki nauyi).

Magana:[1].Sumalatha M, et al.Isoorientin, Mai Zaɓin Inhibitor na Cyclooxygenase-2 (COX-2) daga Tubers na Pueraria tuberosa.Nat Prod Commun.2015 Oktoba; 10 (10): 1703-4.
[2].Ya T, et al.Isoorientin yana haifar da apoptosis, yana rage ɓarna, kuma yana raguwar ɓoyewar VEGF ta hanyar kunna siginar AMPK a cikin ƙwayoyin kansa na pancreatic.Onco Targets Ther.2016 Dec 12;9:7481-7492.
[3].Anilkumar K, et al.Ƙimar Kayayyakin Anti-Inflammatory na Isoorientin Warewa daga Tubers na Pueraria tuberosa.Oxid Med Cell Longev.2017; 2017: 5498054.