Kayayyaki

Manufar:JNK1 jnk2 NF-κ B

Nazarin in vitro:isovitexin yana hana LPS haifar da lalacewar oxidative ta hanyar hana samar da ROS intracellular, kuma yana raunana tasirin H2O2 akan iyawar salula.Ya ƙunshi LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) ba shi da cytotoxicity zuwa raw 264.7 Kwayoyin, amma 200 μ G / ml isovitexin ya nuna muhimmancin cytotoxicity.Isovitexin (25,50) μ G / ml) ya hana LPS haifar da TNF-α, Ƙara yawan matakan IL-6, iNOS da COX-2.Isovitexin (25,50) μ G / ml) kuma ya hana I a cikin raw 264.7 sel κ B α Phosphorylation da lalata, wanda ya dace da tasirin JNK1 / 2 inhibitor [1].

A cikin nazarin vivo:isovitexin (50 da 100 mg / kg, IP) ya haifar da ƙananan canje-canje na histopathological a cikin sassan huhu kuma ya rage adadin ƙwayoyin kumburi a cikin ƙwayoyin cuta na LPS.Heteroeosinophils (50 da 100 mg / kg, IP) sun rage TNF by- α Kuma IL-6 samar da, ROS samar, MPO da MDA abun ciki, ƙara SOD da GSH, da kuma hana LPS jawo kumburi da oxidative danniya a LPS jawo Ali mice.Kuma yadda ya kamata ya hana bayyanar furotin na iNOS da COX-2 [1].Isovitexin (25,50, 100 mg / kg) ya rage yawan rayuwa na LPS / D-gal wanda ya haifar da raunin hanta a cikin mice ta hanyar dogaro da kashi.Isovitexin kuma ya hana NF-κ B kuma ya daidaita LPS / D-gal Nrf2 da HO-1 a cikin mice [2].

Gwajin kwayar halitta:An ƙaddara yiwuwar tantanin halitta ta hanyar MTT assay.Raw 264.7 Kwayoyin An allura a kan 96 rijiyoyin faranti (1) × 104 Kwayoyin / rijiyar) kuma tare da daban-daban yawa na isovitexin (karshen taro: 0-200) μ G / ml) da LPS (2 μ G / ml) na 24 hours.Bugu da kari, amfani da IV (25 ko 50 μG / ml) pretreated da sel na 1 hour, sa'an nan H 2O 2 (300) da aka kara μ M） Bayan 24 hours, MTT (5 mg / ml) aka kara zuwa ga Kwayoyin sa'an nan kuma a sanya su na tsawon awanni 4 [1].

Gwajin dabba:mice [1] don kafa ƙirar Ali, an raba mice ba da gangan zuwa ƙungiyoyi 6: sarrafawa (saline), kawai isovitexin (100 mg / kg, narkar da 0.5% DMSO), kawai LPS (0.5 mg / kg, narkar da cikin saline). LPS (0.5 mg / kg) + isovitexin (50 ko 100 mg / kg) da LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, narkar da a cikin Saline).Isovitexin ko DEX (5 mg / kg) an gudanar da isovitexin.Bayan bayyanar isovitexin ko DEX na awa 1, an yi amfani da berayen tare da ether kuma an gudanar da LPS cikin ciki (a) don haifar da rauni na huhu.An kashe dabbobin sa'o'i 12 bayan gudanar da LPS.Sabili da haka, ruwan lavage ruwa na bronchoalveolar (BALF) da samfuran nama na huhu an girbe don auna matakan cytokine;ROS tsara;SOD, GSH, MDA da ayyukan MPO;Kuma bayanin COX-2, iNOS, HO-1 da Nrf2 sunadaran [1].