Kayayyaki

Bayani:
prim-o-glucosylcimifugin yana da tasirin anti-mai kumburi.Maganar iNOS da COX-2 za a iya hana su ta hanyar daidaita siginar JAK2 / STAT3

Rukunin da suka dace:
Hanyar sigina > > rigakafi da kumburi > > Nitric oxide synthase
Filin bincike > > kumburi / rigakafi
Kayayyakin halitta > > wasu

Manufar:iNOS
COX-2

A cikin Nazarin Vitro:
prim-o-glucosylglycine (POG) shine mafi girman abun ciki na chromone kuma ɗayan manyan abubuwan da ke aiki a cikin Fangfeng (RS).Prim-o-glucosylglycine yana taka rawar anti-mai kumburi a cikin raw 264.7 macrophages ta hanyar hana jigilar siginar JAK2 / STAT3 da hana bayyanar iNOS da COX-2.An auna cytotoxicity na prim-o-glucoside akan LPS da aka kunna raw 264.7 macrophages.Yi amfani da LPS (1 μ G / ml) da haɓaka ƙididdiga na prim-o-glucosylglycine (15, 50 da 100 μ G / ml) na awanni 24, kuma an kimanta yiwuwar tantanin halitta ta hanyar CCK-8 assay.Idan aka kwatanta da ƙwayoyin DMSO da aka kula da su (iko), 24 hours da fallasa zuwa 15-100 μ Bayan g / ml prim-o-glucoside, yiwuwar tantanin halitta ba ta da tasiri sosai.Don nazarin tasirin anti-mai kumburi na prim-o-glucosylglycine, ko prim-o-glucosylglycine na iya shafar NO kira da aka bincika a cikin LPS kunna raw 264.7 Kwayoyin.Yi amfani da LPS (1 μ G / ml) da yawa daban-daban na prim-o-glucosylglycine (15, 50 da 100 μG / ml) na awanni 24.Ba a auna maida hankali a cikin al'adar da ta fi ƙarfin hali ta hanyar Griess.Matsakaicin babu a cikin al'adar al'ada ya karu sosai tare da bayyanar LPS, kuma prim-o-glucoside ya hana LPS da yawa ya haifar da babu samarwa ta hanyar dogaro da hankali [1].
A cikin Nazarin Vivo
An tattara ruwan lavage ruwa na bronchoalveolar (BALF) 7 hours bayan gudanar da lipopolysaccharide (LPS), kuma an auna matakin cytokine a cikin BALF ta ELISA.Idan aka kwatanta da ƙungiyar kulawa, TNF a cikin BALF- α, IL-1 β Kuma matakan IL-6 sun karu sosai.Duk da haka, pretreatment tare da prime-o-glucosylglycine (2.5, 5 ko 10 mg / kg) da muhimmanci saukar da TNF-α, IL-1 β Da IL-6 a cikin wani kashi-dogara hanya (P <0.05, P <0.05, 0.01). ) [1].

Gwajin Kwayoyin Halitta
An yi amfani da Kit ɗin ƙidaya tantanin halitta (CCK-8) don ƙayyade ƙwayar cytotoxic na prim-o-glucosylglycine.A takaice dai, an yi amfani da danyen sel 264.7 da 1 kowace rijiya × An yi allurar sel guda 104 a cikin rijiyoyi 96 kuma an sanya su cikin dare.Sa'an nan kuma amfani da 1 μ Kwayoyin an motsa su tare da g / ml LPS kuma ana bi da su tare da nau'i-nau'i na prim-o-glucosylglycine (15, 50 da 100 μ g / ml; Medchem express, Princeton, NJ, USA) ko DMSO magani.Bayan shiryawa a 37 ℃ na 24 hours, CCK-8 bayani da aka kara a kowace rijiya da kuma incubated na wani sa'a.An auna absorbance a 450 nm ta amfani da mai karanta microplate [1].

Gwajin Dabbobi
beraye [1] BALB/C mazan beraye, masu tsawon makonni 8, suna auna kusan 18 zuwa 20g.An raba berayen bazuwar zuwa ƙungiyoyi 5: ƙungiyar kulawa;Ƙungiyar LPS;LPS + prime-o-glucosylglycine (2.5, 5 ko 10mg / kg nauyin jiki).Prime-o-glucosylglycine an gudanar da shi ta intraperitoneally.Bayan awa 1, beraye a cikin rukunin LPS da ƙungiyar LPS + prime-o-glucosylglycine an ba su 50 mg / L intranasal (a) (200 mg / L) μ LLPs don haifar da mummunan rauni na huhu.An ba da berayen sarrafawa 50% intranasal (a) ba tare da LPS μ LPBS ba
[1]

Magana:
[1].Zhou J, et al.Prim-O-glucosylcimifugin Yana Haɓaka Amsar Cutar Cutar Lipopolysaccharide a cikin RAW 264.7 Macrophages.Pharmacogn Mag.2017 Jul-Satumba; 13 (51): 378-384.
[2].Chen N, et al.Prime-O-glucosylcimifugin yana rage raunin huhu da lipopolysaccharide ke haifarwa a cikin mice.Int Immunopharmacol.2013 Juni; 16 (2): 139-47.