Kayayyaki

Shirye-shiryen bayani:Ɗauki adadin daidaitaccen bayani na Ruscogenin, auna shi daidai, kuma ƙara methanol don sanya shi dauke da 50% a kowace 1ml μG bayani. ml na maganin magana, sanya su a cikin kwandon kwandon shara tare da tasha bi da bi, kuma a kwashe sauran ƙarfi a cikin wanka na ruwa.Daidai ƙara 10ml perchloric acid, girgiza shi da kyau, ajiye shi a cikin ruwan zafi na tsawon minti 15, fitar da shi, sanyaya shi da ruwan kankara, ɗaukar reagent mai dacewa a matsayin fanko, auna absorbance a tsawon 397nm bisa ga ultraviolet bayyane spectrophotometry ( Shafi VA), ɗauki abin sha azaman mai daidaitawa da maida hankali azaman abscissa, kuma zana madaidaicin lanƙwasa.

Shirye-shiryen maganin gwaji:a sha kamar 3G lafiya foda na samfurin, a auna shi daidai, sanya shi a cikin kwandon kwandon shara tare da tasha, daidai 50ml na methanol, auna shi, zafi da reflux na tsawon awa 2, kwantar da shi, auna shi, gyara nauyin da ya ɓace. tare da methanol, girgiza shi da kyau sannan a tace.Sai a auna 25ml na ci gaba da tacewa daidai sai a saka a cikin filo, sai a dawo da kaushi ya bushe, sai a zuba ruwa 10ml a narkar da ragowar, sai a jika shi da ruwa, a girgiza shi da n-butanol sau 5, 10ml kowane lokaci, sai a hada n. - Maganin butanol, a wanke shi sau biyu tare da maganin ammonia, 5ml kowane lokaci, jefar da maganin ammonia, sannan a kwashe maganin n-butanol don bushewa.Narkar da ragowar da methanol 80% da kuma canja shi zuwa 50ml volumetric flask, ƙara 80% methanol zuwa sikelin kuma girgiza da kyau.

Hanyar ƙaddara daidai gwargwado 2 ~ 5ml na maganin gwajin, sanya shi a cikin bututun gwajin busasshen 10ml, bisa ga hanyar da ke ƙarƙashin shirye-shiryen daidaitaccen lanƙwasa, auna ɗaukar kamar yadda doka ta tanada daga "volatilizing da sauran ƙarfi a cikin wanka na ruwa", karanta adadin ruscoegenin a cikin maganin gwajin daga daidaitaccen lanƙwasa kuma lissafta shi.

Jimlar saponins na Ophiopogon japonicus ba zai zama ƙasa da 0.12% bisa Ruscogenin (C27H42O4).

Yanayi na chromatographic: (don tunani kawai)