Kayayyaki

Typhon glycoside

Puhuang shine busasshiyar pollen na Typha angustifolia L., Typha Orientalis Presl ko irin tsire-tsire masu kama a cikin Typhaceae.Abubuwan da ke cikin isorhamnetin-3-o-neohesperidin a cikin Pharmacopoeia na Sinanci (Bugu na 2005) an ƙaddara ta HPLC.Babban hanyoyin da za a tabbatar da isorhamnetin-3-o-neohesperidin a cikin Typhae da shirye-shiryensa sune.

HPLC.Ƙarƙashin ƙayyadaddun abun ciki na Typha a cikin Pharmacopoeia na Sinanci (Bugu na 2005):

Yanayi na chromatographic da gwajin amfani da tsarin: octadecyl silane bonded silica gel as filler;An yi amfani da ruwan Acetonitrile (15:85) azaman lokacin hannu;Tsayin da aka gano shine 254 nm.Yanayin zafin jiki 40 ℃.Yawan faranti na ka'idar ba zai zama ƙasa da 1000 ba bisa ga kololuwar isorhamnetin-3-o-neohesperidin.

Shirye-shiryen maganin gwaji: ɗauki kimanin 0.5g na wannan samfurin, auna shi daidai, saka shi a cikin kwalban aunawa na 50ml, ƙara 45ml na methanol, maganin ultrasonic (ikon 250W, mita 20KHz) na minti 30, kwantar da shi, ƙara methanol zuwa ruwa. ma'auni, girgiza shi da kyau, tace shi, kuma ɗauka ci gaba da tacewa

Ƙaddamar da isorhamnetin-3-o-neohesperidin a Puhuang da shirye-shiryensa ta HPLC.Babban tsarin lokaci na wayar hannu shine ruwan acetonitrile:

① Hp1050 babban aikin ruwa chromatograph;Shafin Chromatographic C18 (5) μ m,4.6mm × 250mm) Cibiyar Dalian na Kimiyyar Kimiyyar Kimiyya, Kwalejin Kimiyya ta kasar Sin);Lokacin wayar hannu: ruwan acetonitrile (15:85);Tsawon tsayin tsinkaya: 254nm;Rukunin greenhouse zafin jiki.

② Babban aikin chromatograph na ruwa shine tsarin hp1050 na Kamfanin Hewlett Packard a Amurka;Chromatographic ginshiƙi bondclone 10 C18 (3.9mm) × 300mm) ; Waya lokaci: ruwa acetonitrile (80:20);tsayin daka na gano 254 nm, bandwidth 4 nm;550 nm tsawon tunani, bandwidth 100 nm;atamfa 8.

Fu Daxu et al.Ƙaddara abubuwan da ke cikin typhoniflorin da isorhamnetin-3-o-neohesperidin a cikin jimlar flavone tsantsa na Typha ta HPLC da 1100 chromatograph.Rukunin chromatographic shine husufin XdB C18 (250mm × 4.6mm, 5 μ m) ； Lokacin wayar hannu: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15: 78: 7; 15min: 23: 70: 7);Tsawon tsayin da aka gano shine 248 nm.Shiri na jimlar flavonoids tsantsa daga Typha: 50g na Typha aka cire tare da 60% ethanol (500ml) × 2), 1H kowane lokaci, da tsantsa aka mayar da hankali a 40 ℃, da tsantsa da aka narkar da da 600ml ruwa da centrifuged.An narkar da ragowar tare da 300ml na ruwa da centrifuged.Hada maganin centrifugal, gyara ƙarar zuwa 1000ml da ruwa, haɗa shi a kan ginshiƙi na guduro macroporous (150ml macroporous guduro busassun nauyi 75g) tare da adadin ɗanyen mai sau 1.5, ƙara 600ml na ruwa da 450ml na 20% ethanol don cirewa. najasa, sa'an nan elute tare da 600ml na 50% ethanol, kuma eluent yana samuwa ta hanyar bushewa a 40 ℃.

Yang Yonghua et al.An yi amfani da babban aikin capillary electrophoresis da babban aikin ruwa chromatography don tantance abubuwan da ke cikin isorhamnetin 232o2, neohesperidin da typhoside a Puhuang.Hpce-3d high yi capillary electrophoresis kayan aiki (Hewlett Packard, Amurka), ma'adini capillary shafi 50 μm × 56cm (HP), lc-6a high yi ruwa chromatograph (Shimadzu kamfanin na Japan), C18 shafi 5mm × 200mm, barbashi size 5 μ M (kamfanin Dalian Yite).HPCE yanayi: buffer 0.02mol/l borax-0.05mol/l 10% acetonitrile na sodium dodecyl sulfate (SDS), ƙarfin lantarki 20kV, shafi zafin jiki 30 ℃, ganewa kalaman 270nm, allura girma 30KPa · s.Halin HPLC: tsarin wayar hannu shine ruwan acetonitrile (15:85), zafin jiki na ginshiƙi shine 25 ℃, kuma tsayin tsinkayar ya kasance 254 nm.

Abubuwan Magana na Typhaneoside

[suna]typhonioside [1]

[CAS No.]104472-68-6

[hanyar ganowa]HPLC ≥ 98%

[Takaddun shaida]20mg 50mg 100mg 500mg 1g (za a iya kunshe bisa ga abokin ciniki bukatun)

[kayayyaki]amorphous foda