Verbascoside CAS Lamba 61276-17-3
Bayani mai mahimmanci
[suna]Mullein glycoside
[lalata]ergosterol, Mullein
[kategori]phenylpropanoid glycosides
[sunan Ingilishi]acteoside;Verbascoside;Kusaginin
[Molecular formula]Saukewa: C29H36O15
[nauyin kwayoyin halitta]624.59
[CAS No.]61276-17-3
Properties na Physicochemical
[kayayyaki]wannan samfurin farin allura crystal foda
[yawan dangi]1.6g/cm 3
[soluble]mai sauƙi mai narkewa a cikin ethanol, methanol da ethyl acetate.
Tushen hakar
Wannan samfurin shine busasshen nama mai bushewa tare da ɓawon ganye na Cistanche deserticola, tsiron dangin liedang.
Hanyar Gwaji
HPLC ≥ 98%
Yanayi na chromatographic: lokacin wayar hannu methanol acetonitrile 1% acetic acid (15:10:75), yawan kwarara 0.6 ml · min-1, shafi zafin jiki 30 ℃, tsinkayar kalaman 334 nm (don tunani kawai)
Aiki da Amfani
Ana amfani da wannan samfurin don tantance abun ciki
Hanyar Ajiya
2-8 ° C, adana daga haske.
Bioactivity na Verbascoside
A cikin Nazarin Vitro:
A matsayin m PKC Inhibitor na ATP, Verbascoside yana da IC50 na 25 μ M. Verbascoside ya nuna kis na 22 da 28 dangane da ATP da histone, bi da bi μM. [1]
A cikin Nazarin Vivo:
Verbascoside (1%) ya rage abubuwan da ke faruwa na gabaɗayan ɓarna da kuma tsananin raunukan fata a cikin ƙirar linzamin kwamfuta na 2,4-dinitrochlorobenzene (DNCB) - haifar da atopic dermatitis (AD).Verbascoside kuma na iya toshe pro-mai kumburi cytokine TNF a cikin DNCB haifar da raunuka fata- α, Maganar IL-6 da IL-4 mRNA [2].Verbascoside (50100 mg / kg, IP) bai canza yanayin sanyi mara kyau ba wanda ya haifar da rauni na matsawa na yau da kullun (CCI).Verbascoside (200 mg / kg, IP) ya rage rashin lafiyar sanyi ga acetone mai motsa jiki a ranar 3. Verbascoside kuma ya rage yawan canje-canjen halayen da ke hade da neuropathy.Bugu da ƙari, Verbascoside ya rage Bax kuma ya karu Bcl-2 a ranar 3 [3].
Gwajin Kwayoyin Halitta:
Lymphocytic mouse leukemia L1210 Kwayoyin (ATCC, CCL 219) sun ƙunshi 10% fetal bovine serum, 4 mM glutamine, 100 U / ml penicillin, 100 μ A cikin 24 rijiyar gungu farantin Dulbecco modified Eagle matsakaici, 104 Kwayoyin kowane riji da aka spanly laidly. Ml streptomycin sulfate da Verbascoside (narkar da su a cikin DMSO).An kula da girma ta hanyar kirga adadin ƙwayoyin sel a cikin Coulter counter bayan kwanaki 2 na shiryawa a cikin yanayi mai ɗanɗano (5% CO2 a cikin iska) a 37 ℃.An ƙididdige ƙimar IC50 bisa ga layin koma baya na linzamin da aka kafa don kowane fili na gwaji [1].
Gwajin Dabbobi:
Domin haifar da atopic dermatitis (AD) - kamar alamomi, berayen [2] sunyi amfani da 2,4-dinitrochlorobenzene (DNCB).A takaice dai, an cire gashin dorsal na beraye tare da almakashi na lantarki kwanaki 2 kafin maganin DNCB.Za 200 μ L na 1% DNCB (a cikin acetone: man zaitun = 4: 1) an shafa shi ga fatar baya da aka aske don sanin yakamata.An sake kai hare-hare a wuri guda, 0.2% DNCB kowane kwanaki 3 na kimanin makonni 2.An raba mice zuwa ƙungiyoyin 4 (n = 6 a kowace ƙungiya): (1) kulawar abin hawa, (2) DNCB da aka bi da shi kawai, (3) 1% Verbascoside (acetone: man zaitun 4: 1) - magani kawai, da ( 4) DNCB + 1% Verbascoside da aka kula da ƙungiyar[2].
Magana:
[1].Herbert JM, et al.Verbascoside keɓe daga Lantana camara, mai hana furotin kinase C.J Nat Prod.1991 Nuwamba-Dec; 54 (6): 1595-600.
[2].Li Y, et al.Verbascoside Yana Rage Atopic Dermatitis-Kamar Alamu a cikin Mice ta Ƙarfin Ƙarfafa Ƙwararru.Int Arch Allergy Immunol.2018; 175 (4): 220-230.
[3].Amin B, et al.Tasirin Verbascoside a cikin Ciwon Neuropathic wanda ya haifar da Rauni na Tsanani a cikin Rats.Phytother Res.2016 Jan; 30 (1): 128-35.
