ʻO Glycyrrhizin, Liquiritin;Liquiritoside;Likviritin;Liquiritoside Cas No.551-15-5
Hoʻolauna pōkole
ʻO Glycyrrhizin, i kapa ʻia ʻo Liquiritin.He mea kanu ʻo Licorice o Glycyrrhiza ma Leguminosae.ʻO kona mau aʻa a me kona ʻōpuʻu he mau lāʻau Kina maʻamau.
Hoʻolaha nui ʻia ka lāʻau lapaʻau ma Northeast Kina, Xinjiang, Yunnan, Inner Mongolia, Anhui a me nā wahi ʻē aʻe.Ua helu ʻo Shennong materia medica classic iā ia ma ka papa kiʻekiʻe, me ka ʻōlelo ʻana "ʻo kēia mauʻu ke aliʻi o nā lāʻau lapaʻau āpau, a he kakaikahi ka poʻe ʻaʻole hoʻohana ia".Loaʻa i ka Licorice nā mea paʻakikī, ʻo ka hapa nui me nā triterpenoids, flavonoids a me nā coumarins.ʻO Flavonoids kekahi ʻano mea bioactive i loaʻa mai ka licorice extract.ʻO kāna mau mea lāʻau lapaʻau nui ka glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, etc. I nā makahiki i hala iho nei, ua loaʻa nā hōʻike e pili ana i ka free radical scavenging, antioxidant, anti-cancer a me nā hopena anti mutagenic o nā flavonoids licorice ma ka home a ma waho.
KemikaName:4H-1-Benzopyran-4-one, 2- [4-(β-D-glucopyranosyloxy) phenyl]-2, 3-dihydro-7-hydroxy-, (S)
Pkino kinoPkuleana:Monohydrate (dilute ethanol a i ʻole ka wai), ka helu heheʻe: 212 ~ 213 ° ℃.
Hana lāʻau lapaʻau
Kaona: ʻAʻole
Ka hopena ʻino: ʻike ʻole
Kumu kumu: legume Glycyrrhiza glabra L. root, Glycyrrhiza uralensis Fisch Root.
Ka unuhi ʻana o Glycyrrhizin
Hoomaamaa mua ana i na mea Laiki
He paʻakikī loa ka haku mele ʻana o ka licorice maka.I mea e loaʻa ai ka hopena hoʻokaʻawale ʻoi aku ka maikaʻi, e hoʻemi i ka haumia o nā haumia ma ka kolamu chromatographic hoʻomākaukau, a hoʻomaikaʻi i ka ʻike o ka glycyrrhizin i loko o ka injection raw material, ua hoʻohana ʻia ke ʻano unuhi e hoʻomaʻamaʻa i ka mea maka.E kaupaona i ka mauʻu 4G me kahi koena uila a hoʻokomo i loko o ka beaker.E ana pono i ka 100ml wai i hoʻomaʻemaʻe ʻia me ke ana cylinder a ninini i loko o ka beaker no ka hemo ʻana.Kani ultrasonic no ma kahi o 15 minuke, a hoʻoulu mau me ke aniani koʻokoʻo e accelerate dissolution.A laila e hoʻokomo i ka beaker i loko o kahi ʻauʻau thermostatic 90 ℃ a hoʻomehana iā ia no 2 mau hola, a laila wela ia no ka kānana.Ma hope o ka hoʻohui ʻana i ka kānana i loko o ka solvent n-butanol a kū no kekahi mau minuke, ua hoʻoheheʻe ʻia ka hapa nui o nā glycosides i loko o ka solvent n-butanol, a laila e hana i ke kiʻekiʻe kiʻekiʻe, e unuhi i kahi liʻiliʻi o nā glycosides i koe i loko o ka wai, a hope e hui a hoʻopaʻa i ka n- ʻO ka hopena butanol i loaʻa ma ke kiʻi lua no ka chromatography a me ka hoʻomaʻemaʻe.
Hoʻomaʻemaʻe o Glycyrrhizin e Chromatography
E lawe i 10 ml o ka huahana i unuhi ʻia ma luna nei ma ke ʻano he mea ʻokoʻa, e hoʻomaka i ka paila, e hoʻonohonoho i ka kahe kahe ma 25 ml / min, a lawe mai i ka mea maka i loko o 500 mm e ka pae kelepona (methanol: wai = 1:4) × I kahi 40 mm kolamu hoʻomākaukau, e hōʻiliʻili i ka hakina o ka hay glucoside huahana e like me ke kūlana kiʻekiʻe: ʻo ka hapa o ka 1 h mua e hōʻiliʻili pū ʻia e like me ka hapa mua o ka haumia, a laila hoʻololi i ke kahe.No ka laʻana, holoi i ke kolamu me ka hui ʻana o 50% methanol a me ka wai, e hoʻopili i ka huahana i kēlā me kēia 20 min, a laila e hoʻopaʻa i kēlā me kēia omole o ka huahana me ka rotary evaporation, a lawe i 20 µ L no ka HPLC chromatographic analysis, A hiki ʻole ke ʻike ʻia kahi pahuhopu.ʻO nā kūlana ʻike o ka HPLC penei: ka pae kelepona: methanol: wai = 3.5:6.5;ʻO ka pae paʻa: silica gel carbon 18;kolamu Chromatographic: 450 mm × 4.6 mm ； Kahe kahe: 1 ml / min;Ka lōʻihi nalu ʻike: 254nm.ʻO ka ʻike o ka glycyrrhizin i ka lua o ka hue ka mea kiʻekiʻe loa ma waena o nā huahana i loaʻa i kēlā me kēia 20 min
Hoʻomaʻemaʻe o Glycyrrhizin e Rechromatography
No ka mea ʻaʻole kiʻekiʻe ka ʻike o ka glycyrrhizin ma hope o ka hoʻomaʻemaʻe chromatographic mua, ua koho ʻia ke ʻano like.E lawe i ka 10 ml o ka mea i hoʻomaʻemaʻe ʻia ma luna e like me ka mea makaʻala, ʻo ka kahe kahe ʻana he 25 ml / min, a lawe mai i ka lua o ka hue o ka huahana i 500 mm e ka mobile phase (methanol: wai = 2:5) × I ka 20 mm. } kolamu chromatographic, e ohi i ka distillate o ka hay glycoside huahana e like me ke kūlana kiʻekiʻe: hoʻohui i ka huahana i kēlā me kēia 4 min, a laila e hoʻopololei i kēlā me kēia omole huahana me ka rotary evaporation, a e hoʻohana i ka ʻāpana ʻike like ma luna no ka nānā ʻana chromatographic HPLC a hiki i ka loaʻa ʻole o ka pahuhopu. .Ma hope o ka nānā ʻana, ua ʻike ʻia ʻo ka maʻiʻo o ka glycyrrhizin i ke ono o ka hue ka mea kiʻekiʻe loa ma waena o nā huahana i loaʻa i kēlā me kēia 4 min, kahi o ka manawa paʻa he 5.898 min e like me ke kiʻekiʻe kiʻekiʻe, a hiki i ka maʻiʻo ma kahi o 40% ma ke ʻano maʻamau. .
Mahope o ka lapaau ana i na huahana
Hoʻopili ʻia ka huahana i hōʻiliʻili ʻia ma lalo o ke kaomi hoʻemi ʻia ma kahi evaporator rotary ma 70 ℃.Ma hope o ka hoʻoheheʻe ʻia ʻana o ka mea hoʻoheheʻe, e hoʻoheheʻe i ka huahana paʻa ma ka ipu poʻe o lalo me kahi nui o ka methanol, a crystallize i loko o kahi paipu hoʻāʻo ma ka lumi wela a hiki i ka ʻike ʻia ʻana o nā kristal keʻokeʻo keʻokeʻo [2].
