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wehewehe:ʻO isoorientin kahi mea hoʻopiʻi COX-2 maikaʻi me kahi waiwai IC50 o 39 μM.

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Pahuhopu: cox-2:39 μ M (IC50)

Nā haʻawina in vitro:ʻO Isoorientin kahi mea paʻa koho o ka cyclooxygenase-2 (COX-2) mai ka ʻōpala o Pueraria tuberosa [1].Ua mālamaʻia nā pūnaewele PANC-1 a me patu-8988 me Isoorientin (0,20,40,80 a me 160 μ M) E ulu i mua o nā hola 24 a hoʻohui i ka hopena CCK8.Ma ka 20, 40, 80 a me 160 μ Ma ka hoʻonuiʻiaʻana o M, ua emi nui ka olaola pūnaewele. Ua hoʻohanaʻiaʻo Isoorientin (0,20,40,80 a me 160) no nā pūnaewele μ M no PANC-1;Ua hoʻohana ʻia ka 0, 20, 40, 80160 a me 320 μ M no ka moʻomeheu patu-8988) no 24 mau hola, a ua loiloi ʻia ka ʻōlelo o P e Western blot - AMPK a me AMPK.Ua hoʻonui ʻia ka ʻōlelo o ka p-ampk ma hope o ka mālama ʻana iā Isoorientin.A laila, i loko o ka hui shRNA, 80 μ M ka manaʻo e ʻike i ka hopena o Isoorientin.ʻOi aku ka haʻahaʻa o nā pae haʻiʻōlelo o AMPK a me p-ampk i ka hui shRNA ma mua o ka poʻe i loko o nā cell PC wild-type (WT) a me ka hui i hoʻololi ʻia me ka lentivirus mana maikaʻi ʻole (NC) [2].

Nā haʻawina ma vivo:ʻO nā holoholona i mālama ʻia me Isoorientin ma 10 mg / kg a me 20 mg / kg ke kaumaha o ke kino, he hōʻemi koʻikoʻi koʻikoʻi i ka edema claw, me ka mānoanoa peak o 1.19 ± 0.05 mm a me 1.08 ± 0.04 mm.Ua hōʻike kēia i ka Isoorientin i hōʻemi nui i ka edema paw i hoʻohālikelike ʻia me ka pūʻulu mana [3].

ʻO ka hoʻokolohua kelepona:ʻO PANC-1 a me patu-8988 pūnaewele i hoʻopaʻa ʻia ma 96 mau papa punawai.Loaʻa i kēlā me kēia pūnāwai he ~ 5000 mau pūnaewele a me 200 mau cell μ L me ka 10% FBS.I ka hiki ʻana o nā cell i loko o kēlā me kēia pūnāwai i ka hui ʻana o 70%, ua hoʻololi ʻia ka medium a ua hoʻohui ʻia ʻo FBS manuahi me nā ʻokoʻa like ʻole o ka isoorientin.Ma hope o nā hola 24, holoi ʻia nā cell i hoʻokahi manawa me ka PBS, ua hoʻolei ʻia ka moʻomeheu moʻomeheu i loko o ka isoorientin, a ua hoʻohui ʻia ka 100% μL FBS manuahi manuahi a me 10 μL cell helu helu kit 8 (CCK8) reagent.Hoʻokomo ʻia nā cell ma 37 ℃ no kekahi mau hola 1-2, a ua ʻike ʻia ka absorbance o kēlā me kēia pūnāwai ma 490 nm me ka hoʻohana ʻana i kahi mea heluhelu ELISA.Hōʻike ʻia ke ola o ke kelepona ma ke ʻano he hoʻololi nui i ka absorbance [2].

Hoʻokolohua holoholona:i ka hihia o ka paw edema kŘkohu, iole [3] i haawi ia i ka isoorientin a i ole celecoxib intraperitoneally, a ua hookomo pono ia ka carrageenan i ka paw hookahi hora ma hope.I loko o ke kŘkohu airbag, komo pololei nā lā'au lapa'au a pau i ka lua o ka 'eke me ka carrageenan.Ua hoʻokomo ʻia ka isoorientin i 3 mau hola ma mua o ka hoʻokomo ʻia ʻana o ka carrageenan i loko o ka capsule.Hāʻawi ʻia ʻo Isoorientin a me celecoxib i nā ʻiole.Ua hoʻomākaukau ʻia nā hoʻonā waiwai o isoorientin (100 mg / ml) a me celecoxib (100 mg / ml) i DMSO a hoʻoheheʻe hou ʻia i ka wā mālama.Ua hoʻokaʻawale ʻia nā holoholona i ʻelima mau hui like ʻole: ka mālama ʻana (DMSO mālama ʻia);Carrageenan mālama ʻia (0.5 ml (1.5% (w / V) carrageenan i loko o ka brine); carrageenan mālama ʻia + celecoxib (20mg / kg paona kino); carrageenan mālama ʻia + isoorientin (10 mg / kg kino kaumaha); mālama i ka carrageenan + isoorientin (20mg / kg kaumaha kino).

Kuhikuhi:[1].Sumalatha M, et al.ʻO Isoorientin, kahi mea pale koho o Cyclooxygenase-2 (COX-2) mai nā Tuber o Pueraria tuberosa.Nat Prod Commun.2015 ʻOkakopa;10(10):1703-4.
[2].Ye T, et al.Hoʻoulu ʻo Isoorientin i ka apoptosis, hoʻemi i ka invasiveness, a hoʻohaʻahaʻa i ka huna ʻana o VEGF ma o ka hoʻoulu ʻana i ka hōʻailona AMPK i nā pūnaewele maʻi maʻi pancreatic.ʻO Onco Targets Ther.2016 Dec 12;9:7481-7492.
[3].Anilkumar K, et al.ʻO ka loiloi o nā ʻano Anti-Inflammatory Properties o Isoorientin i hoʻokaʻawale ʻia mai nā Tuber o Pueraria tuberosa.Oxid Med Cell Longev.2017;2017:5498054.