Nā huahana

wehewehe:
ʻO ka pachymic acid he triterpenoid mai P. cocos.ʻO ka pachymic acid ke kāohi i nā ala hōʻailona Akt a me ERK.

Māhele pili:
kahua noiʻi > > maʻi 'aʻai
Nā Huahana Kūlohelohe > > terpenoids a me nā glycosides

Pahuhopu:
Akt
ERK

Haʻawina In Vitro:
hiki i ka pachymic acid (PA) ke keakea i ka tumorigenesis o ka ma'i ma'i gallbladder e pili ana i na ala hoailona Akt a me ERK.ʻO ka lāʻau pachymic acid (PA) i pāpā nui ʻia i nā ala Rho a, Akt a me ERK i loko o nā pūnaʻi maʻi maʻi gallbladder.Hiki i ka lāʻau pachymic acid (PA) ke hoʻohaʻahaʻa i ka PCNA, ICAM-1, RhoA, p-Akt a me ka perk ma kahi ʻano hilinaʻi.12 mau hola ma hope o ka mālama ʻana, 10 μG / ml Poria acid (PA) i kāohi i ka ulu ʻana o ka cell ma kahi ʻano o 30 μG / ml i hōʻemi hou i ka ulu ʻana o ke kelepona.Ua kāohi ʻia ka ulu ʻana o ka cell i kahi manawa - a me ke ʻano hilinaʻi.Ma hope o 48 mau hola o ka mālama ʻana, ʻo 10 μg/mL, 20 μG / ml, 20 μG / ml a me 30 μ Pachymic acid (PA) i ka g / ml i kāohi i ka ulu ʻana o ka cell ma kahi o 25%, 40% a me 70%.ʻO ka pachymic acid (PA) hoʻi e kāohi i ka ulu ʻana o nā maʻi maʻi maʻi gallbladder ma kahi ʻano e hilinaʻi ana i ka manawa a me ka hopena.

Ma ke aʻo ʻana ma Vivo:
i mea e loiloi ai i ka hana antitumor o ka pachymic acid (PA) i loko o ka vivo, ua hoʻohana ʻia ke ʻano hoʻohālike xenograft tumo kanaka nci-h23.ʻO ka pachymic acid (PA) ka mea i kāohi nui i ka ulu ʻana o ka maʻi maʻi ma nā ʻano o 30 a me 60 mg / kg no 21 mau lā i hoʻohālikelike ʻia me ka pūʻulu mana [2].

ʻO ka hoʻokolohua kelepona:
Ua hoʻohana ʻia ka pahu helu helu-8 (CCK-8) e loiloi i ka hopena anti proliferation o ka pachymic acid (PA) ma nā pūnaewele GBC-SD.I ka pōkole, ma hope o ka mālama ʻana, e hoʻohui i ka 10 i kēlā me kēia lua μ Lcck-8 solution, a ma hope o hoʻokahi incubation, ua ana ʻia ka absorbance ma 450 nm me ka hoʻohana ʻana i kahi microplate heluhelu [1].

Hoʻokolohua holoholona:
ʻiole [2] hoʻohana i nā ʻiole athymic olohelohe wahine he 4-5 mau pule.ʻO nā pūnaewele nci-h23 ulu nui (ma 100 μ5 ma LPBs × 106) i hoʻokomo ʻia i loko o ka ʻaoʻao ʻākau o kēlā me kēia ʻiole.Ua hoʻonuiʻia nā xenografts Tumor i ka awelika nui o 100-200mm 3 a ua hāʻawiʻia i nā hui likeʻoleʻehā (6 mauʻiole i kēlā me kēia hui): (a) kaʻa kaʻa (0.1% DMSO i ka saline maʻamau);（b）ʻakika pachymic（PA）10 mg / kg;（c）PA 30 mg / kg;（Dd）PA 60mg/kg。 Ua hāʻawi ʻia nā ʻiole pa e ka intraperitoneal (IP) injection no 3 pule (5 lā / pule).Ua ana ʻia ka nui o ka ʻōpū ma luna o ʻelua koʻi me ke kōkua o ka vernier caliper, a ua helu ʻia ka nui o ka tumo (mm3).

Kuhikuhi:
[1].ʻO Chen Y, et al.ʻO ka pachymic acid ka mea e pale ai i ka tumorigenesis i loko o nā pūpū carcinoma gallbladder.Int J Clin Exp Med.2015 ʻOkakopa 15;8(10):17781-8.
[2].Ma J, et al.Hoʻokomo ka pachymic acid i ka apoptosis ma o ka hoʻoulu ʻana i nā ala koʻikoʻi JNK a me ER i hilinaʻi ʻia ROS i loko o nā pūnaʻi maʻi kanesa.ʻO ka Cancer Cell Int.2015 ʻAukake 5;15:78.