Paeoniflorin CAS No. 23180-57-6
ʻIke Pono
ʻIke ʻia ʻo paeoniflorin, he pinane monoterpene bitter glycoside i hoʻokaʻawale ʻia mai ka peony ʻulaʻula a me ka peony keʻokeʻo.He pauka amorphous hygroscopic.Aia ia ma nā aʻa o Paeonia, peony, peony poni a me nā mea kanu ʻē aʻe o Ranunculaceae.He haʻahaʻa loa ka ʻona o kēia kristal.
[inoa kemika]5beta-[(Benzoyloxy)methyl]tetrahydro-5-hydroxy-2-methyl-2,5-methano-1H-3,4-dioxacyclobuta[cd] pentalen-1alpha(2H)-yl-beta-D-glucopyranoside
[kumu molekala]C23H28O11
【CASʻAʻole】23180-57-6
Maʻemaʻe: ma luna o 98%, ke ala ʻike: HPLC.
[kumu]ʻO nā aʻa o Paeonia albiflora pall, P. suffrsticosa Andr, P. delarayi Franch, he mea kanu o Ranunculaceae, ʻo ka ʻike o ka radix paeoniae Rubr ka mea kiʻekiʻe loa.
[Hōʻike]10%，20%，30%，50%，90% ， 98%
[ApiliImea komo ] ʻO ka huina glucosides o Paeonia (TGP) ka inoa nui o paeoniflorin, hydroxy paeoniflorin, paeoniflorin, albiflorin a me benzoyl paeoniflorin, i kapa ʻia ʻo TGP no ka pōkole.
ʻAno kino a me kemika
He hygroscopic amorphous Tan pauda (90% he pauda keʻokeʻo) [α] 16D-12.8. (C = 4.6, methanol), ʻo ka tetraacetate he aniani acicular kala ʻole, kahi heheʻe: 196 ℃.Paʻa ʻo Paeoniflorin i loko o ke kaiapuni acidic (pH 2 ~ 6) a paʻa ʻole i ka ʻāina alkaline.
Hoʻoholo maʻiʻo
ʻO ka maʻamau, hiki ke hoʻohana ʻia ke ala 1 a me ke ala 2 no ka ʻike.Hoʻohana maikaʻi ʻia ke ʻano 1 no ka hana ʻana i nā maʻiʻo kiʻekiʻe, hiki ke kōkua i ka hana ʻana i nā limahana e hoʻoholo maikaʻi i ka maʻemaʻe o nā huahana.He maʻalahi ka hoʻoheheʻe ʻana o ka mea kuhikuhi ma hope o ka hemo ʻana.
1. Ua hoʻoholo ʻia e ka chromatography wai hana kiʻekiʻe (Appendix VI d).Ua hoʻāʻo ʻia nā kūlana chromatographic a me ke kūpono o ka ʻōnaehana me Octadecyl silane paʻa silica gel i mea hoʻopiha;Ua hoʻohana ʻia ʻo Acetonitrile-0.1% phosphoric acid solution (14:86) ma ke ʻano o ka pae kelepona;ʻO 230nm ka lōʻihi hawewe ʻike.ʻAʻole e emi ka nui o nā papa manaʻo e like me ka paeoniflorin peak ma mua o 2000. Ka hoʻomākaukau ʻana i ka hopena kuhikuhi: e kaupaona pono i ka nui kūpono o ka paeoniflorin reference solution a hoʻohui i ka methanol e hoʻomākaukau ai i ka 60% paeoniflorin no 1ml μG solution.
2. No ka hoʻomaikaʻi ʻana i ke ʻano o ka paeoniflorin ma Radix Paeoniae Alba.Nā ʻano hana: ua hoʻohālikelike ʻia nā ʻano hana ma Chinese Pharmacopoeia a me nā ʻano i hoʻomaikaʻi ʻia.ʻO ka wai methanol (30:70) ka pae kelepona a ʻo 230nm ka lōʻihi o ka hawewe.hopena;Maikaʻi ka pilina laina o kēia ʻano hana (r = 0.9995).ʻO ka hoʻihoʻi maʻamau ʻo 101.518% a ʻo RSD ka 1.682%.Ka hopena: he maʻalahi a pololei ke ala i hoʻomaikaʻi ʻia, hiki ke hōʻemi i ka ʻawaʻawa o nā mea hoʻoheheʻe organik i nā kānaka a me ka pollution kaiapuni, a hāʻawi i kahi kumu kuhikuhi no ka hoʻoholo ʻana o ka paeoniflorin i ka hana.
Hoʻoholo Hoʻoholo
Ka hoʻoholo ʻana o ka paeoniflorin e HPLC
Ka laulā o ka noi:Ke hoʻohana nei kēia ʻano hana i ka HPLC e hoʻoholo i ka ʻike o ka paeoniflorin i loko o nā paila Guizhi Fuling.
He kūpono ke ala no ka pill Guizhi Fuling.
Kuʻina kumu:e hoʻokomo i ka hāpana hoʻāʻo i loko o kahi pahu conical, e hoʻohui i kahi nui kūpono o ka ethanol dilute no ka unuhi ʻana i ke kani ultrasonic, hoʻoluʻu, hoʻoluliluli maikaʻi, kānana, komo ka filtrate i ka chromatograph wai hana kiʻekiʻe no ka hoʻokaʻawale chromatographic, hoʻohana i ka mea ʻike hoʻoheheʻe ultraviolet e ʻike i ka waiwai absorption o paeoniflorin ma ka nalu nalu o 230nm, a e helu i kona maʻiʻo.
