Nā huahana

ʻO Typhonine glycoside

ʻO Puhuang ka pollen maloʻo o Typha angustifolia L., Typha Orientalis Presl a i ʻole nā ​​mea kanu like i ka Typhaceae.Hoʻoholo ʻia ka ʻike o ka isorhamnetin-3-o-neohesperidin ma Chinese Pharmacopoeia (2005 Edition) e HPLC.ʻO nā ala nui no ka hoʻoholo ʻana i ka isorhamnetin-3-o-neohesperidin ma Typhae a me kāna hoʻomākaukau ʻana.

HPLC.Ma lalo o ka manaʻo hoʻoholo o Typha ma Chinese Pharmacopoeia (2005 Edition):

Nā kūlana Chromatographic a me ka hoʻāʻo ʻana i ka hoʻohana ʻana i ka ʻōnaehana: octadecyl silane i hoʻopaʻa ʻia i ka silica gel i mea hoʻopiha;Ua hoʻohana ʻia ka wai acetonitrile (15:85) ma ke ʻano o ka pae kelepona;ʻO 254 nm ka lōʻihi nalu.Kona wela 40 ℃.ʻAʻole e emi ka helu o nā papa theoretical ma mua o 1000 e like me ka piko o isorhamnetin-3-o-neohesperidin.

Ka hoʻomākaukau ʻana i ka hopena hoʻāʻo: e lawe ma kahi o 0.5g o kēia huahana, e kaupaona pono iā ia, e hoʻokomo iā ia i loko o kahi hue ana 50ml, e hoʻohui i 45ml o ka methanol, ka lāʻau ultrasonic (mana 250W, alapine 20KHz) no 30 mau minuke, e hoʻomaha, e hoʻohui i ka methanol i ka unahi, lulu maikaʻi, kānana, a lawe i ka kānana mau

ʻO ka hoʻoholo ʻana o ka isorhamnetin-3-o-neohesperidin ma Puhuang a me kāna hoʻomākaukau ʻana e HPLC.ʻO ka wai acetonitrile ka ʻōnaehana pae kelepona maʻamau:

① Hp1050 kiʻekiʻe hana wai chromatograph;Chromatographic kolamu C18 (5) μ m,4.6mm × 250mm) Dalian Institute of Chemical Physics, Chinese Academy of Sciences);ʻĀpana kelepona: wai acetonitrile (15:85);Ka lōʻihi o ka nalu ʻike: 254nm;ʻO ke kolamu wela wela.

② ʻO ka chromatograph wai hana kiʻekiʻe he hp1050 ʻōnaehana o Hewlett Packard Company ma ʻAmelika Hui Pū ʻIa;Chromatographic kolamu bondclone 10 C18 (3.9mm) × 300mm； Ke pae paʻa: wai acetonitrile (80:20);ka lōʻihi nalu ʻike 254 nm, bandwidth 4 nm;ka lōʻihi hawewe kuhikuhi 550 nm, bandwidth 100 nm;hoemi 8.

ʻO Fu Daxu et al.Hoʻoholo i nā mea o typhoniflorin a me isorhamnetin-3-o-neohesperidin i ka huina flavone extract o Typha e HPLC a me 1100 chromatograph.ʻO ke kolamu chromatographic ʻo eclipse XdB C18 (250mm × 4.6mm，5 μm) ； ʻĀpana kelepona: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15:78:7; 15min: 23:70:7);ʻO 248 nm ka lōʻihi hawewe ʻike.Ka hoomakaukau ana o ka huina flavonoids extract o Typha: 50g o Typha ua extracted me 60% ethanol (500ml) × 2), 1H i kela a me keia manawa, ka extract ua concentrated ma 40 ℃, ka extract ua hemo me ka 600ml wai a me centrifuged.Ua hoʻoheheʻe ʻia ke koena me 300ml o ka wai a centrifuged.Hoʻohui i ka hopena centrifugal, hoʻoponopono i ka leo i ka 1000ml me ka wai, adsorb ma luna o ke kolamu o ka resin macroporous (150ml macroporous resin maloʻo kaumaha 75g) me 1.5 manawa o ka nui o ka lāʻau lapaʻau, elute me 600ml o ka wai a me 450ml o 20% ethanol e wehe. nā haumia, a laila elute me 600ml o 50% ethanol, a loaʻa ka eluent ma ka maloʻo maloʻo ma 40 ℃.

ʻO Yang Yonghua et al.Hoʻohana ʻia ka electrophoresis capillary kiʻekiʻe a me ka chromatography wai hana kiʻekiʻe e hoʻoholo i nā mea o ka isorhamnetin 232o2, neohesperidin a me ka typhoside ma Puhuang.Hpce-3d kiʻekiʻe hana capillary electrophoresis mea hana (Hewlett Packard, USA), quartz capillary kolamu 50 μm × 56cm (HP), lc-6a kiʻekiʻe hana wai chromatograph (Shimadzu hui o Iapana), C18 kolamu 5mm × 200mm, ʻāpana nui 5 μ M (Dalian Yite hui).Nā kūlana HPCE: buffer 0.02mol/l borax-0.05mol/l 10% acetonitrile o ka sodium dodecyl sulfate (SDS), voli 20kV, kolamu wela 30 ℃, loaʻa hawewe ʻike 270nm, ka nui injection 30KPa · s.ʻO nā kūlana HPLC: ʻo ka pae kelepona he wai acetonitrile (15:85), ʻo ka mahana o ke kolamu he 25 ℃, a ʻo ka lōʻihi o ka nalu ʻike he 254 nm.

Mea Hoʻohālikelike Typhaneoside

[inoa]typhonioside [1]

[CAS No.]104472-68-6

[ʻano ʻike]HPLC ≥ 98%

[Hōʻike]20mg 50mg 100mg 500mg 1g (hiki ke hoʻopili ʻia e like me nā koi o ka mea kūʻai aku)

[nā waiwai]pauda amorphous