Glycyrrhizin, Liquiritin;Liquiritoside;Likviritin;Liquiritoside Cas No.551-15-5
Lus Taw Qhia
Glycyrrhizin, tseem hu ua Liquiritin.Licorice yog ib tsob nroj ntawm Glycyrrhiza hauv Leguminosae.Nws cov hauv paus hniav thiab stems yog suav tshuaj ntsuab.
Tshuaj yog dav faib nyob rau hauv Northeast Tuam Tshoj, Xinjiang, Yunnan, Inner Mongolia, Anhui thiab lwm qhov chaw.Shennong materia medica classic sau nws raws li qib siab, hais tias "cov nyom no yog huab tais ntawm txhua yam tshuaj, thiab muaj tsawg tus uas tsis siv nws".Licorice muaj cov khoom sib txuam, feem ntau suav nrog triterpenoids, flavonoids thiab coumarins.Flavonoids yog ib hom bioactive Cheebtsam tau los ntawm licorice extract.Nws cov khoom siv tshuaj lom neeg feem ntau suav nrog glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, thiab lwm yam. Nyob rau hauv xyoo tas los no, muaj cov lus ceeb toom ntawm cov dawb radical scavenging, antioxidant, anti-cancer thiab anti mutagenic teebmeem ntawm licorice nyob rau hauv txawv teb chaws.
TshuajName:4H-1-Benzopyran-4-one, 2- [4-(β-D-glucopyranosyloxy) phenyl]-2, 3-dihydro-7-hydroxy-, (S)
PhysicalPvaj tse:Monohydrate (dilute ethanol los yog dej), melting point: 212 ~ 213 ° ℃.
Pharmacological Action
Toxicity: Tsis muaj
Cov tshuaj tiv thaiv tsis zoo: tsis paub
Cov khoom xyaw: legume Glycyrrhiza glabra L. cag, Glycyrrhiza uralensis Fisch Root.
Extraction ntawm Glycyrrhizin
Pretreatment Ntawm Licorice Raw Materials
Cov tshuaj muaj pes tsawg leeg ntawm licorice raw khoom yog heev complex.Txhawm rau kom tau txais kev sib cais zoo dua, txo cov pa phem ntawm impurities ntawm kev npaj chromatographic kem, thiab txhim kho cov ntsiab lus ntawm glycyrrhizin hauv cov khoom siv raw, cov txheej txheem rho tawm tau siv los ua cov khoom siv raw.Ntsuas 4G quav nyab nrog hluav taws xob sib npaug thiab muab tso rau hauv lub beaker.Ua kom raug ntsuas 100ml dej distilled nrog lub ntsuas lub tog raj kheej thiab ncuav nws mus rau hauv lub beaker kom yaj.Ultrasonic rau li 15 feeb, thiab tas li do nrog ib tug iav pas nrig kom ceev dissolution.Tom qab ntawd muab lub khob rau hauv 90 ℃ thermostatic da dej thiab ua kom sov rau 2 teev, ces sov nws rau pom.Tom qab ntxiv cov filtrate rau hauv n-butanol hnyav thiab sawv ntsug rau ob peb feeb, feem ntau ntawm glycosides yog yaj hauv n-butanol hnyav, tom qab ntawd ua cov txheej txheem thib ob, rho tawm ib qho me me ntawm glycosides nyob hauv dej, thiab thaum kawg ua ke thiab mloog zoo rau n- butanol tov tau los ntawm kev rho tawm theem nrab rau chromatography thiab purification.
Purification ntawm Glycyrrhizin los ntawm Chromatography
Siv 10 ml ntawm cov khoom rho tawm saum toj no raws li cov khoom siv raw khoom, pib lub twj tso kua mis, teeb tsa tus nqi ntws ntawm 25 ml / min, thiab nqa cov khoom siv raw rau hauv 500 hli los ntawm theem mobile (methanol: dej = 1: 4) × Hauv ib qho 40 mm npaj kem, sau cov feem ntawm quav nyab glucoside cov khoom raws li lub ncov qhov teeb meem: cov feem ntawm thawj 1 h yog sau ua ke raws li ib tug pre impurity feem, thiab ces hloov lub ntws.Piv txwv li, ntxuav lub kem nrog sib tov ntawm 50% methanol thiab dej, txuas cov khoom txhua txhua 20 min, thiab tom qab ntawd mloog zoo rau txhua lub raj mis ntawm cov khoom nrog rotary evaporation, thiab siv 20 µ L rau HPLC chromatographic tsom xam, kom txog thaum tsis muaj lub hom phiaj.Cov kev kuaj pom ntawm HPLC yog raws li nram no: mobile theem: methanol: dej = 3.5:6.5;Nyob ruaj ruaj theem: silica gel carbon 18;Chromatographic kem: 450 mm × 4.6 mm; Flow tus nqi: 1 ml / min;Ntsuas wavelength: 254nm.Cov ntsiab lus ntawm glycyrrhizin hauv lub raj mis thib ob yog qhov siab tshaj plaws ntawm cov khoom tau txais txhua 20 feeb
Purification ntawm Glycyrrhizin los ntawm Rechromatography
Txij li thaum cov ntsiab lus ntawm glycyrrhizin tom qab thawj chromatographic purification tsis siab, tib txoj kev raug xaiv.Siv 10 ml ntawm cov khoom purified saum toj no raws li cov khoom siv raw khoom, cov nqi ntws yog 25 ml / min, thiab nqa lub raj mis thib ob ntawm cov khoom rau hauv 500 hli los ntawm theem mobile (methanol: dej = 2: 5) × Hauv 20 hli. } chromatographic kem, sau cov distillate ntawm quav nyab glycoside cov khoom raws li lub ncov qhov teeb meem: txuas cov khoom txhua txhua 4 min, ces mloog zoo rau txhua lub raj mis ntawm cov khoom nrog rotary evaporation, thiab siv tib lub nrhiav kom tau strip saum toj no rau HPLC chromatographic tsom mus txog rau thaum tsis muaj lub hom phiaj. .Tom qab kev soj ntsuam, nws tau pom tias cov ntsiab lus ntawm glycyrrhizin nyob rau hauv lub thib rau lub raj mis yog qhov siab tshaj ntawm cov khoom tau txais txhua txhua 4 min, nyob rau hauv uas lub sij hawm tuav yog 5.898 min raws li lub hom phiaj ncov, thiab cov ntsiab lus mus txog 40% los ntawm cheeb tsam normalization txoj kev. .
Tom Qab Kev Kho Cov Khoom Siv
Cov khoom uas tau sau tseg yog distilled nyob rau hauv txo lub siab ntawm rotary evaporator ntawm 70 ℃.Tom qab cov kuab tshuaj yog qhov pib evaporated, yaj cov khoom ntawm lub khob hauv qab nrog methanol me me, thiab crystallize nyob rau hauv ib lub raj ntsuas ntawm chav tsev kub kom txog thaum dawb granular crystals tshwm [2].
