Cov khoom

Hom phiaj:JNK1 jnk2 NF- κ B

Kev tshawb nrhiav hauv vitro:isovitexin tiv thaiv LPS induced oxidative puas tsuaj los ntawm inhibiting zus tau tej cov intracellular ROS, thiab kuj tsis muaj zog cov nyhuv ntawm H2O2 ntawm cell viability.Muaj LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) tsis muaj cytotoxicity rau raw 264.7 hlwb, tab sis 200 μ G / ml isovitexin pom cytotoxicity tseem ceeb.Isovitexin (25,50) μ G / ml) inhibited LPS induced TNF- α, nce qib ntawm IL-6, iNOS thiab COX-2.Isovitexin (25,50) μ G / ml) kuj inhibited I hauv raw 264.7 hlwb κ B α Phosphorylation thiab degradation, uas zoo ib yam nrog cov nyhuv ntawm JNK1 / 2 inhibitor [1].

Hauv vivo kev tshawb fawb:isovitexin (50 thiab 100 mg / kg, IP) ua rau tsis tshua muaj kev hloov pauv histopathological hauv lub ntsws thiab txo cov hlwb inflammatory hauv LPS induced nas.Heteroeosinophils (50 thiab 100 mg / kg, IP) txo TNF by- α Thiab IL-6 ntau lawm, ROS ntau lawm, MPO thiab MDA cov ntsiab lus, nce SOD thiab GSH, thiab tiv thaiv LPS induced o thiab oxidative stress nyob rau hauv LPS induced Ali nas.Thiab zoo inhibit qhov kev qhia ntawm cov protein ntawm iNOS thiab COX-2 [1].Isovitexin (25,50, 100 mg / kg) txo qis kev ciaj sia ntawm LPS / D-gal induced siab raug mob hauv cov nas nyob rau hauv koob tshuaj.Isovitexin kuj inhibit NF- κ B thiab nce tswj LPS / D-gal induced Nrf2 thiab HO-1 hauv nas [2].

Kev sim ntawm tes:cell viability tau txiav txim los ntawm MTT kev xeem.Raw 264.7 hlwb raug inoculated rau ntawm 96 zoo daim hlau (1) × 104 hlwb / zoo) thiab nrog ntau yam concentrations ntawm isovitexin (kawg concentration: 0-200) μ G / ml) thiab LPS (2 μ G / ml) rau 24 teev.Tsis tas li ntawd, siv IV (25 lossis 50 μ G / ml) pretreated cov hlwb rau 1 teev, thiab tom qab ntawd H 2O 2 (300) tau ntxiv μ M). Tom qab 24 teev, MTT (5 mg / ml) tau ntxiv rau hauv cell thiab ces incubated rau 4 teev [1].

Tsiaj kev sim:nas [1] txhawm rau tsim Ali qauv, nas tau muab faib ua 6 pawg: tswj (saline), tsuas yog isovitexin (100 mg / kg, yaj hauv 0.5% DMSO), tsuas yog LPS (0.5 mg / kg, yaj hauv saline). ), LPS (0.5 mg / kg) + isovitexin (50 lossis 100 mg / kg) thiab LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, yaj hauv saline).Isovitexin lossis DEX (5 mg / kg) isovitexin tau muab tshuaj.Tom qab kis rau isovitexin lossis DEX rau 1 teev, nas tau muab tshuaj loog nrog ether thiab LPS tau muab tshuaj intranasally (hauv) txhawm rau ua rau mob ntsws.Cov tsiaj tau euthanized 12 teev tom qab LPS tswj hwm.Yog li, cov kua dej bronchoalveolar lavage (BALF) thiab cov qog nqaij hlav ntsws tau raug sau los ntsuas cov qib cytokine;ROS tiam;SOD, GSH, MDA thiab MPO cov haujlwm;Thiab kev qhia ntawm COX-2, iNOS, HO-1 thiab Nrf2 proteins [1].