Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
Cov ntaub ntawv tseem ceeb
Salvianolic acid B yog condensation ntawm peb molecules ntawm Danshensu thiab ib tug molecule ntawm caffeic acid.Nws yog ib qho ntawm ntau txoj kev tshawb fawb salvianolic acids.Nws muaj cov tshuaj pharmacological tseem ceeb rau lub plawv, hlwb, siab, raum thiab lwm yam kabmob.Cov khoom no muaj cov txiaj ntsig ntawm kev txhawb nqa cov ntshav ncig thiab tshem tawm cov ntshav stasis, dredging meridians thiab ua kom cov khoom lag luam.Nws yog tsuas yog siv los kho ischemic stroke los ntawm cov ntshav stasis thaiv meridians, xws li loog ntawm ib nrab ntawm lub cev thiab ceg ceg, tsis muaj zog, mob contracture, lub cev tsis muaj zog, qhov ncauj thiab qhov muag deflection, thiab lwm yam.
Alias:salvianolic acid B, salvianolic acid B, salvianolic acid B
Lub npe Askiv:salvianolic acid B
Molecular formula:c36h30o 16
Molecular Luj:718.62 ib
CAS Nr:.115939-25-8
Kev kuaj xyuas txoj kev:HPLC ≥ 98%
Specifications:10mg, 20mg, 100mg, 500mg, 1g (tuaj yeem ntim tau raws li cov neeg siv khoom xav tau)
Muaj nuj nqi thiab siv:Cov khoom no yog siv rau kev txiav txim siab cov ntsiab lus.
Lub cev thiab tshuaj muaj zog
Khoom:cov khoom yog quasi dawb hmoov.
Cov saj yog me ntsis iab thiab astringent, nrog noo noo inducing khoom.Soluble hauv dej, ethanol thiab methanol.
Salvianolic acid B yog tsim los ntawm condensation ntawm 3 molecules ntawm salvianolic acid thiab 1 molecule ntawm caffeic acid.Nws muaj ob pawg carboxyl thiab muaj nyob rau hauv daim ntawv ntawm cov ntsev sib txawv (K +, Ca2 +, Na +, NH4 +, thiab lwm yam).Nyob rau hauv tus txheej txheem ntawm Decoction thiab concentration, ib feem me me ntawm salvianolic acid B yog hydrolyzed rau ntshav oxalic acid thiab salvianolic acid, thiab ib feem ntawm salvianolic acid B ua rosmarinic acid nyob rau hauv acidic tej yam kev mob;Salvianolic acid A thiab C tuaj yeem ua tautomeric hauv kev daws.
Specifications
5%, 10%, 50%, 70%, 90%, 98%
Kev rho tawm
Radix Salviae Miltiorrhizae tau crushed, muab tso rau hauv lub tank extraction, soaked nrog 8 npaug ntawm tus nqi ntawm 0.01mol / l hydrochloric acid thaum hmo ntuj, thiab ces percolated nrog 14 npaug ntawm cov dej.Cov tshuaj percolated extracted yog purified los ntawm AB-8 macroporous resin kem.Ua ntej, elute nrog 0.01mol / l hydrochloric acid kom tshem tawm cov tsis adsorbed impurities, thiab ces elute nrog 25% ethanol kom tshem tawm cov polar impurities heev.Thaum kawg, mloog zoo rau 40% ethanol eluent nyob rau hauv lub siab txo kom rov qab ethanol thiab khov-qhuav kom tau tag nrho Salvia miltiorrhiza phenolic acid nrog ib tug purity ntawm ntau tshaj 80%.
Txheeb xyuas
Noj 1g ntawm cov khoom, zom nws, ntxiv 5ml ntawm ethanol, tag nrho do, lim, noj ob peb tee ntawm filtrate, dot nws ntawm lub lim ntawv strip, qhuav nws, saib nws nyob rau hauv lub ultraviolet teeb (365nm), qhia xiav- Grey fluorescence, dai cov ntawv lim hauv cov tshuaj ammonia concentrated lub raj mis (tsis hu rau cov kua dej), tshem tawm tom qab 20 feeb, saib nws hauv qab lub teeb ultraviolet (365nm), qhia xiav-ntsuab fluorescence.
Acidity:noj cov tshuaj aqueous nyob rau hauv cov khoom ntawm clarity, thiab tus nqi pH yuav tsum yog 2.0 ~ 4.0 (appendix ntawm Suav Pharmacopoeia 1977 tsab).
Kev txiav txim siab txog cov ntsiab lus
Nws tau txiav txim siab los ntawm kev ua haujlwm siab ua kua chromatography (Appendix VI D, Volume I, Suav Pharmacopoeia, 2000 EDITION).
Octadecyl silane bonded silica gel tau siv los ua tus muab tub lim hauv chromatographic tej yam kev mob thiab kev siv qhov system;Methanol acetonitrile formic acid dej (30:10:1:59) yog theem mobile;Kev kuaj pom wavelength yog 286 nm.Tus naj npawb ntawm cov ntawv theoretical xam raws li salvianolic acid B ncov yuav tsum tsis pub tsawg dua 2000.
Kev npaj cov tshuaj siv tau raug ntsuas qhov tsim nyog ntawm salvianolic acid B siv tshuaj thiab ntxiv dej kom nws muaj 10% ntawm 1ml μ G tov.
Kev npaj cov tshuaj ntsuam xyuas yuav siv li 0.2g ntawm cov khoom, ua kom raug hnyav, muab tso rau hauv 50ml ntsuas lub raj mis, ntxiv methanol, sonicate rau 20 feeb, txias nws, ntxiv dej rau lub teev, co nws zoo, lim. Nws, ntsuas kom raug 1ml ntawm cov dej tsis tu ncua, muab tso rau hauv 25ml ntsuas lub raj mis, ntxiv dej rau qhov ntsuas, co nws zoo.
Txoj kev txiav txim kom raug absorbs 20% ntawm kev tswj kev daws teeb meem thiab 20% ntawm cov tshuaj ntsuam xyuas μ l.Txhaj nws mus rau hauv cov kua chromatograph rau kev txiav txim.
Pharmacological efficiency
Salvianolic acid B yog condensation ntawm peb molecules ntawm Danshensu thiab ib tug molecule ntawm caffeic acid.Nws yog ib qho ntawm ntau txoj kev tshawb fawb salvianolic acids.Nws muaj cov tshuaj pharmacological tseem ceeb rau lub plawv, hlwb, siab, raum thiab lwm yam kabmob.
Antioxidant
Salvianolic acid B muaj zog antioxidant nyhuv.Kev sim hauv vivo thiab hauv vitro qhia tias salvianolic acid B tuaj yeem tshem tawm cov pa dawb radicals thiab inhibit lipid peroxidation.Nws qhov kev siv zog ntau dua li cov vitamin C, vitamin E thiab mannitol.Nws yog ib qho ntawm cov khoom lag luam paub zoo nrog cov txiaj ntsig zoo tshaj plaws antioxidant kev tshawb fawb Pharmacological qhia tau hais tias salvianolic acid rau kev txhaj tshuaj muaj cov nyhuv antioxidant, inhibits platelet aggregation thiab thrombosis, thiab tuaj yeem ua kom ntev lub sij hawm ciaj sia ntawm cov tsiaj hauv qab hypoxia.Cov txiaj ntsig tau pom tias salvianolic acid rau kev txhaj tshuaj (60 ~ 15mg / kg) tuaj yeem txhim kho lub paj hlwb tsis txaus hauv cov nas nrog cerebral ischemia-reperfusion raug mob, txhim kho tus cwj pwm tsis zoo thiab txo qhov chaw ntawm lub paj hlwb.Muaj qhov sib txawv ntawm cov koob tshuaj siab thiab nruab nrab (60 thiab 30mg / kg);Salvianolic acid rau kev txhaj tshuaj tuaj yeem txhim kho lub paj hlwb puas tsuaj los ntawm FeCl3 induced cerebral ischemia hauv nas ntawm 1, 2 thiab 24 teev tom qab kev tswj hwm, uas tau tshwm sim hauv kev txhim kho kev coj cwj pwm tsis zoo thiab txo qis ntawm cerebral infarction cheeb tsam;Salvianolic acid 40 mg / kg rau kev txhaj tshuaj tiv thaiv kev sib sau ntawm luav platelets tshwm sim los ntawm ADP, arachidonic acid thiab collagen, thiab inhibition tus nqi yog 81.5%, 76.7% thiab 68.9% feem.Salvianolic acid 60 thiab 30mg / kg rau txhaj tshuaj tiv thaiv thrombosis hauv nas;Salvianolic acid 60 thiab 30mg / kg rau kev txhaj tshuaj ua rau lub sijhawm muaj sia nyob ntawm nas nyob rau hauv hypoxia.
Daim ntawv thov kev kho mob
Cov khoom no muaj cov txiaj ntsig ntawm kev txhawb nqa cov ntshav ncig thiab tshem tawm cov ntshav stasis, dredging meridians thiab ua kom cov khoom lag luam.Nws yog tsuas yog siv los kho ischemic stroke los ntawm cov ntshav stasis thaiv meridians, xws li loog ntawm ib nrab ntawm lub cev thiab ceg ceg, tsis muaj zog, mob contracture, lub cev tsis muaj zog, qhov ncauj thiab qhov muag deflection, thiab lwm yam.
Khw
Nyob rau hauv ib qho chaw txias thiab qhuav.
Lub sij hawm siv tau
Ob xyoos.
Txoj kev khaws cia
2-8 ° C, khaws cia rau hauv qhov chaw txias thiab qhuav thiab deb ntawm lub teeb.
Teeb meem xav tau kev saib xyuas
Cov khoom yuav tsum tau khaws cia ntawm qhov kub thiab txias.Yog tias nws raug huab cua ntev ntev, cov ntsiab lus yuav raug txo.
