Cov khoom

Lub npe Askiv:tanshinone IIA
Suav Alias:tanshinone |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA kev ua haujlwm lom neeg

Nqe lus piav qhia:
Tanshinone IIA (Tan IIA) yog ib qho ntawm cov roj soluble tseem ceeb hauv cov hauv paus hniav liab Salvia miltiorrhiza.Tanshinone IIA tuaj yeem inhibit angiogenesis los ntawm kev tsom mus rau cov protein kinase ntawm VEGF / VEGFR2.

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Kev tshawb fawb teb > > kab mob plawv
Khoom ntuj >> Quinones

Hom phiaj:
VEGF/VEGFR2[1]

Kev tshawb fawb hauv Vitro:Cov teebmeem antitumor ntawm tanshinone IIA suav nrog inhibiting qog cell proliferation, cuam tshuam cov qog cell voj voog, txhawb qog cell apoptosis thiab inhibiting qog cell invasion thiab metastasis.Tanshinone IIA muaj kev tiv thaiv kev loj hlob ntawm A549 hlwb: IC50 ntawm tanshinone IIA tom qab 24, 48 thiab 72 teev yog 145.3, 30.95 thiab 11.49, raws li μ M. CCK-8 kev soj ntsuam tau siv los ntsuas tanshinone IIA (2.5 μM) Kev ua haujlwm loj ntawm kev kho A549 hlwb ntawm 24, 48 thiab 72 teev, feem.CCK-8 cov txiaj ntsig tau pom tias tanshinone IIA tuaj yeem cuam tshuam kev loj hlob ntawm A549 hlwb hauv cov koob tshuaj raws sijhawm thiab nyob ntawm lub sijhawm.Qhov tseem ceeb apoptosis thiab kev loj hlob ntawm tes inhibition ntawm A549 hlwb tau pom 48 teev tom qab kev kho tshuaj (qhov kev siv tshuaj yog kwv yees li IC50 tus nqi: tanshinone iia31 ntawm A549) μ M). μ M), nqes tswj cov kev qhia ntawm VEGF thiab VEGFR2 protein nyob rau hauv pab pawg neeg kho tshuaj thiab vector [1].Tanshinone IIA yog ib qho ntawm cov khoom siv ntau tshaj plaws ntawm Salvia miltiorrhiza cag, uas tuaj yeem tiv thaiv H9c2 hlwb los ntawm apoptosis.H9c2 hlwb kho nrog tanshinone IIA inhibited angiotensin II induced apoptosis los ntawm kev tswj cov kev qhia ntawm PTEN (phosphatase thiab tensin homologue).PTEN yog cov qog nqaij hlav uas ua lub luag haujlwm tseem ceeb hauv apoptosis.Tanshinone IIA inhibits angiotensin II (AngII) - induced apoptosis los ntawm kev txo qis kev qhia ntawm phosphatase thiab tensin homologue (PTEN) [2].Tanshinone IIA txo cov protein qhia ntawm EGFR, thiab IGFR thaiv cov PI3K / Akt / mTOR txoj hauv kev hauv plab hnyuv AGS hlwb [3].

Kev sim Cell:A549 hlwb raug suav rau hauv theem logarithmic thiab 6000 hlwb (90 μ L ntim) hauv 96 lub phaj zoo.Yuav 10 μL txawv concentrations ntawm tanshinone IIA (kawg concentrations 80,60,40,30,20,15,10,5 thiab 2.5 μ M) Thiab ADM (kawg concentrations 8,4,2,1,0.5 thiab 0.25 μ M ) Nws tau ntxiv rau hauv pawg tshuaj, thaum pawg tswj tsis zoo (pab pawg neeg nqa khoom) tau ntxiv tsuas yog 10 μ Ldmso lossis ib txwm saline tsis muaj tanshinone IIA lossis Adm. Sib tov cov hlwb nrog CCK-8 reagent (100 μ L / ml nruab nrab) rau lwm 2 teev, thiab qhov nqus tau nyeem ntawm 450 nm siv tus nyeem ntawv microplate.Tus nqi ntawm cov cell proliferation inhibition yog xam raws li cov qauv hauv qab no: proliferation inhibition tus nqi (%) = 1 - [(A1-A4) / (A2-A3)] × 100, qhov twg A1 yog tus nqi OD ntawm pawg sim tshuaj, A2 yog OD tus nqi ntawm pawg tswj hwm dawb paug, A3 yog OD tus nqi ntawm RPMI1640 nruab nrab tsis muaj hlwb, thiab A4 yog tus nqi OD ntawm cov tshuaj nrog tib qhov concentration li A1 tab sis tsis muaj hlwb.Tus nqi IC50 yog xam los ntawm nonlinear regression tsom xam siv graphpad prism software [1], uas sawv cev rau cov tshuaj concentration uas qhia 50% cell loj hlob inhibition.

Siv:[1].Xie J, et al.Cov nyhuv antitumor ntawm tanshinone IIA ntawm kev tiv thaiv kev loj hlob thiab txo qis VEGF / VEGFR2 kev qhia ntawm tib neeg tsis yog me me ntawm lub ntsws cancer A549 cell kab.Acta Pharm Sin B. 2015 Nov;5(6:554-63).
[2].Zhang Z, et al.Tanshinone IIA inhibits apoptosis nyob rau hauv myocardium los ntawm inducing microRNA-152-3p qhia thiab yog li downregulating PTEN.Am J Transl Res.2016 Lub Xya Hli 15;8(7):3124-32.
[3].Su CC, et al.Tanshinone IIA txo qis cov protein qhia ntawm EGFR, thiab IGFR thaiv cov PI3K / Akt / mTOR txoj hauv kev gastric carcinoma AGS hlwb ob leeg hauv vitro thiab hauv vivo.Oncol Rep. 2016 Lub Yim Hli;36(2):1173-9.