Cov khoom

Typhonine glycoside

Puhuang yog cov paj ntoos qhuav ntawm Typha angustifolia L., Typha Orientalis Presl lossis cov nroj tsuag zoo sib xws hauv Typhaceae.Cov ntsiab lus ntawm isorhamnetin-3-o-neohesperidin hauv Suav Pharmacopoeia (2005 Edition) yog txiav txim los ntawm HPLC.Cov txheej txheem tseem ceeb rau kev txiav txim siab ntawm isorhamnetin-3-o-neohesperidin hauv Typhae thiab nws cov kev npaj yog

HPLC.Raws li cov ntsiab lus kev txiav txim siab ntawm Typha hauv Suav Pharmocopoeia (2005 Edition):

Chromatographic tej yam kev mob thiab system applicability test: octadecyl silane bonded silica gel li muab tub lim;Acetonitrile dej (15:85) tau siv los ua theem mobile;Qhov ntsuas wavelength yog 254 nm.Kem kub 40 ℃.Tus lej ntawm cov phiaj xwm theoretical yuav tsum tsis pub tsawg dua 1000 raws li qhov siab tshaj ntawm isorhamnetin-3-o-neohesperidin.

Kev npaj cov tshuaj ntsuam xyuas: noj li 0.5g ntawm cov khoom no, ntsuas kom raug, muab tso rau hauv 50ml ntsuas lub raj mis, ntxiv 45ml ntawm methanol, kev kho ultrasonic (hwj chim 250W, zaus 20KHz) rau 30 feeb, txias nws, ntxiv methanol rau lub scale, co nws zoo, lim nws, thiab coj cov nruam filtrate

Kev txiav txim siab ntawm isorhamnetin-3-o-neohesperidin hauv Puhuang thiab nws cov kev npaj los ntawm HPLC.Cov txheej txheem mobile theem yog acetonitrile dej:

① Hp1050 kev ua haujlwm siab ua kua chromatograph;Chromatographic kem C18 (5) μ m, 4.6mm × 250mm) Dalian Institute of Chemical Physics, Suav Academy ntawm Sciences);Mobile theem: acetonitrile dej (15:85);Ntsuas wavelength: 254nm;Kem tsev xog paj kub.

② Kev ua haujlwm siab ua kua chromatograph yog hp1050 system ntawm Hewlett Packard Company hauv Tebchaws Meskas;Chromatographic kem bondclone 10 C18 (3.9mm) × 300mm) ； Txawb theem: dej acetonitrile (80:20);nrhiav wavelength 254 nm, bandwidth 4 nm;siv wavelength 550 nm, bandwidth 100 nm;kev attenuation 8.

Fu Daxu et al.Txiav txim siab cov ntsiab lus ntawm typhoniflorin thiab isorhamnetin-3-o-neohesperidin nyob rau hauv tag nrho flavone extract ntawm Typha los ntawm HPLC thiab 1100 chromatograph.Cov kab chromatographic yog dab noj hnub XdB C18 (250mm × 4.6mm, 5 μ m) ； Txawb theem: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15:78:7; 15min: 23:70:7);Qhov ntsuas wavelength yog 248 nm.Kev npaj tag nrho cov flavonoids extract ntawm Typha: 50g ntawm Typha tau muab rho tawm nrog 60% ethanol (500ml) × 2), 1H txhua zaus, cov extract tau concentrated ntawm 40 ℃, cov extract tau yaj nrog 600ml dej thiab centrifuged.Cov residue yog yaj nrog 300ml dej thiab centrifuged.Muab cov tshuaj centrifugal, kho qhov ntim rau 1000ml nrog dej, adsorb rau ntawm ib kem ntawm macroporous resin (150ml macroporous resin qhuav hnyav 75g) nrog 1.5 npaug ntawm cov tshuaj crude, elute nrog 600ml dej thiab 450ml ntawm 20% ethanol kom tshem tawm. impurities, ces elute nrog 600ml ntawm 50% ethanol, thiab eluent yog tau los ntawm lub tshuab nqus tsev ziab ntawm 40 ℃.

Yang Yonghua et al.Siv kev ua haujlwm siab capillary electrophoresis thiab kev ua haujlwm siab ua kua chromatography los txiav txim siab cov ntsiab lus ntawm isorhamnetin 232o2, neohesperidin thiab typhoside hauv Puhuang.Hpce-3d kev ua haujlwm siab capillary electrophoresis ntsuas (Hewlett Packard, USA), quartz capillary kem 50 μ m × 56cm (HP), lc-6a siab ua kua chromatograph (Shimadzu tuam txhab ntawm Nyiv), C18 kem 5mm × 200mm, particle loj 5 μ M (Dalian Yite tuam txhab).HPCE tej yam kev mob: tsis 0.02mol/l borax-0.05mol/l 10% acetonitrile ntawm sodium dodecyl sulfate (SDS), voltage 20kV, kem kub 30 ℃, nrhiav wavelength 270nm, txhaj ntim 30KPa · s.HPLC tej yam kev mob: lub xov tooj ntawm tes yog acetonitrile dej (15:85), kem kub yog 25 ℃, thiab nrhiav wavelength yog 254 nm.

Typhaneoside Reference khoom

[npe]typhonioside [1]

[CAS Nr.]104472-68-6

[Nrhiav txoj kev]HPLC ≥ 98%

[Specification]20mg 50mg 100mg 500mg 1g (tuaj yeem ntim tau raws li cov neeg siv khoom xav tau)

[cov khoom]amorphous hmoov