Verbascoside CAS No.: 61276-17-3
Cov ntaub ntawv tseem ceeb
[npe]Mullein glycosides
[alias]ergosterol, Mullein
[qeb]phenylpropanoid glycosides
[English npe]acteoside;Verbascoside;Kusaginin
[molecular formula]C29H36O15
[molecular hnyav]624.59 ib
[CAS Nr.]61276-17-3
Physicochemical Properties
[cov khoom]cov khoom no yog dawb koob siv lead ua hmoov
[Cov txheeb ze ntom ntom]1.6g / cm3
[solubility]yooj yim soluble hauv ethanol, methanol thiab ethyl acetate.
Extraction Source
Cov khoom no yog cov qia qhuav nrog scaly nplooj ntawm Cistanche deserticola, ib tsob nroj ntawm Liedang tsev neeg.
Txoj Kev Xeem
HPLC ≥ 98%
Chromatographic tej yam kev mob: mobile theem methanol acetonitrile 1% acetic acid (15:10:75), txaus tus nqi 0.6 ml · min-1, kem kub 30 ℃, nrhiav wavelength 334 nm (rau siv xwb)
Muaj nuj nqi thiab siv
Cov khoom no yog siv rau kev txiav txim siab cov ntsiab lus
Txoj kev khaws cia
2-8 ° C, khaws cia deb ntawm lub teeb.
Bioactivity ntawm Verbascoside
Kev tshawb fawb hauv Vitro:
Raws li kev sib tw PKC Inhibitor ntawm ATP, Verbascoside muaj IC50 ntawm 25 μ M. Verbascoside pom kis ntawm 22 thiab 28 txheeb ze rau ATP thiab histone, raws li μ M. Verbascoside muaj kev ua haujlwm zoo ntawm L-1210 hlwb nrog IC50 ntawm 13 μ M. [1] . Verbascoside (5,10) μ M) Inhibition ntawm 2,4-dinitrochlorobenzene (DNCB) - induced T cell costimulatory yam CD86 thiab CD54, proinflammatory cytokines thiab NF hauv thk-1 hlwb κ B pathway activation [2].
Hauv Vivo Studies:
Verbascoside (1%) txo qhov tshwm sim ntawm kev khawb tag nrho thiab qhov mob ntawm daim tawv nqaij mob hauv tus nas qauv ntawm 2,4-dinitrochlorobenzene (DNCB) - induced atopic dermatitis (AD).Verbascoside tuaj yeem thaiv cov pro-inflammatory cytokine TNF hauv DNCB induced tawv nqaij lesions- α, Kev nthuav qhia ntawm IL-6 thiab IL-4 mRNA [2].Verbascoside (50100 mg / kg, IP) tsis hloov qhov mob khaub thuas txawv txav tshwm sim los ntawm kev raug mob hnyav (CCI).Verbascoside (200 mg / kg, IP) txo qhov ua xua rau qhov mob khaub thuas stimulated acetone nyob rau hnub 3. Verbascoside kuj txo qis tus cwj pwm hloov cuam tshuam nrog neuropathy.Tsis tas li ntawd, Verbascoside txo Bax thiab nce Bcl-2 rau hnub 3 [3].
Kev sim Cell:
lymphocytic nas leukemia L1210 hlwb (ATCC, CCL 219) muaj 10% fetal bovine serum, 4 mM glutamine, 100 U / ml penicillin, 100 μ Hauv 24 qhov zoo pawg phaj ntawm Dulbecco hloov Eagle nruab nrab, 104 lub hlwb ib leeg tau zoo. Ml streptomycin sulfate thiab Verbascoside (ua kua hauv DMSO).Kev loj hlob raug saib xyuas los ntawm kev suav cov xov tooj ntawm tes hauv Coulter txee tom qab 2 hnub ntawm incubation nyob rau hauv ib qho chaw ntub dej (5% CO2 hauv huab cua) ntawm 37 ℃.Tus nqi IC50 raug xam raws li cov kab regression linear tsim rau txhua qhov kev xeem sib xyaw [1].
Kev sim tsiaj:
Yuav kom induce atopic dermatitis (AD) - zoo li cov tsos mob, nas [2] siv 2,4-dinitrochlorobenzene (DNCB).Nyob rau hauv luv luv, cov plaub hau dorsal ntawm nas raug tshem tawm nrog hluav taws xob txiab 2 hnub ua ntej kev kho DNCB.Yuav 200 μ L ntawm 1% DNCB (hauv acetone: txiv roj roj = 4: 1) tau siv rau shaved rov qab daim tawv nqaij rau sensitization.Kev tawm tsam rov ua dua ntawm tib qhov chaw, 0.2% DNCB txhua 3 hnub rau li 2 lub lis piam.Cov nas tau muab faib ua 4 pawg (n = 6 hauv txhua pab pawg): (1) kho tsheb tswj, (2) DNCB kho nkaus xwb, (3) 1% Verbascoside (acetone: txiv roj roj 4: 1) - kho nkaus xwb, thiab ( 4) DNCB + 1% Verbascoside kho pab pawg[2].
Siv:
[1].Herbert JM, et al.Verbascoside cais los ntawm Lantana camara, ib qho inhibitor ntawm protein kinase C. J Nat Prod.1991 Nov-Dec; 54(6): 1595-600.
[2].Li Y, et al.Verbascoside Alleviates Atopic Dermatitis-zoo li cov tsos mob hauv cov nas los ntawm nws cov muaj zog tiv thaiv kab mob.Int Arch Allergy Immunol.2018; 175(4): 220-230.
[3].Amin B, et al.Cov nyhuv ntawm Verbascoside nyob rau hauv Neuropathic Mob Induced los ntawm Chronic Constriction Injury nyob rau hauv nas.Phytother Res.2016 Jan; 30(1:128-35).
