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Nkọwa:isoorientin bụ ihe mgbochi COX-2 dị irè yana uru IC50 nke 39 μM.

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Ogige nyocha > > Ọrịa cancer eke > > flavonoids
Ogige nyocha > > mbufụt / mgbochi
Ebumnuche: cox-2:39 μM (IC50)

Ọmụmụ in vitro:Isoorientin bụ onye na-egbochi cyclooxygenase-2 (COX-2) site na tuber nke Pueraria tuberosa [1].A na-agwọ sel PANC-1 na patu-8988 na Isoorientin (0,20,40,80 na 160 μ M) Na-eto n'ihu maka awa 24 ma gbakwunye CCK8 ngwọta.Na 20, 40, 80 na 160 μ Na ntinye uche nke M, mkpụrụ ndụ cell na-ebelata nke ukwuu. A na-eji Isoorientin (0,20,40,80 na 160) mee ihe maka sel μ M maka PANC-1;0, 20, 40, 80160 na 320 μM ka ejiri mee ihe maka patu-8988) omenala maka awa 24, na Western blot tụlere okwu P - AMPK na AMPK.Okwu p-ampk mụbara mgbe ọgwụgwọ Isoorientin gasịrị.Mgbe ahụ, na otu shRNA, ntinye 80 μM iji chọpụta mmetụta nke Isoorientin.Ọkwa ngosipụta nke AMPK na p-ampk dị na otu shRNA dị ala karịa ndị nọ n'ụdị sel PC anụ ọhịa (WT) wee jiri lentivirus njikwa na-adịghị mma (NC) bufee otu ahụ.

Ọmụmụ ihe na vivo:Anụmanụ ejiri ọgwụ Isoorientin emeso ya na 10 mg / kg na 20 mg / kg arọ ahụ nwere mbelata dị ịrịba ama nke edema n'ụkwụ, yana ọkpụrụkpụ kacha elu nke 1.19 ± 0.05 mm na 1.08 ± 0.04 mm, n'otu n'otu.Nke a gosiri na Isoorientin belatara nke ukwuu edema paw ma e jiri ya tụnyere ndị na-achịkwa [3].

Nnwale cell:A gbanyere sel PANC-1 na patu-8988 n'efere olulu mmiri iri itoolu na isii.Mmiri nke ọ bụla nwere ~ sel 5000 na sel 200 μ L nwere 10% FBS.Mgbe mkpụrụ ndụ dị na nke ọ bụla ruru 70% confluence, a gbanwere usoro ahụ yana agbakwunyere FBS free media yana ntinye dị iche iche nke isoorientin.Mgbe awa 24 gachara, a na-eji PBS sachaa mkpụrụ ndụ ahụ otu ugboro, tụfuo usoro ọdịnala nwere isoorientin, tinyekwa 100% μ L FBS free media na 10 μ L cell counting kit 8 (CCK8) reagent.A na-etinye mkpụrụ ndụ ahụ na 37 ℃ maka awa 1-2 ọzọ, a chọpụtakwara nnabata nke olulu mmiri ọ bụla na 490 nm site na iji onye na-agụ ELISA.A na-egosipụta ike ndụ cell dị ka ọtụtụ mgbanwe na nnabata [2].

Nnwale anụmanụ:N'ihe banyere ụdị edema paw, a na-enye ụmụ oke [3] isoorientin ma ọ bụ celecoxib intraperitoneal, na carrageenan na-agbanye ozugbo n'ime paw otu awa ka e mesịrị.Na ụdị airbag, ọgwụgwọ niile na-abanye n'ime oghere akpa ozugbo na carrageenan.A na-agbanye isoorientin awa 3 tupu etinye carrageenan n'ime capsule.A na-enye Isoorientin na celecoxib maka ụmụ oke.A kwadebere ihe ngwọta nke isoorientin (100 mg / ml) na celecoxib (100 mg / ml) na DMSO wee gbasaa ọzọ n'oge ọgwụgwọ.E kewara anụmanụ ndị a n'ime otu ise dị iche iche: njikwa (DMSO mesoro);A na-agwọ carrageenan (0.5 ml (1.5% (w / V) carrageenan na brine); A na-agwọ carrageenan + celecoxib (20mg / kg arọ ahụ); carrageenan + isoorientin na-agwọ (10 mg / kg arọ ahụ); Na-emeso carrageenan + isoorientin (20mg / kg). n'arọ arọ).

Ntụaka:[1].Sumalatha M, et al.Isoorientin, onye na-egbochi Cyclooxygenase-2 (COX-2) sitere na Tubers nke Pueraria tuberosa.Nat Prod Commun.2015 Ọkt; 10 (10): 1703-4 .
[2].Ee T, et al.Isoorientin na-ebute apoptosis, na-ebelata mbuso agha, ma na-agbadata nzuzo VEGF site na ịgbalite akara AMPK na mkpụrụ ndụ kansa pancreatic.Onco Targets Ther.2016 Dec 12;9:7481-7492 .
[3].Anilkumar K, et al.Nyochaa Njirimara Mgbochi nke Isoorientin Kewapụrụ na Tubers nke Pueraria tuberosa.Oxid Med Cell Longev.2017;2017:5498054.