Ngwaahịa

Ebumnuche:JNK1 jnk2 NF-κ B

Ọmụmụ in vitro:isovitexin na-egbochi mmebi oxidative nke LPS site na igbochi mmepụta nke intracellular ROS, ma na-ebelata mmetụta nke H2O2 na ndụ cell.Nwere LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) enweghị cytotoxicity na mkpụrụ ndụ 264.7 raw, mana 200 μ G / ml isovitexin gosipụtara cytotoxicity dị ịrịba ama.Isovitexin (25,50) μ G / ml) na-egbochi LPS na-ebute TNF-α, Ọkwa ụba nke IL-6, iNOS na COX-2.Isovitexin (25,50) μ G / ml) na-egbochikwa m na mkpụrụ ndụ 264.7 raw κ B α phosphorylation na mbibi, nke kwekọrọ na mmetụta nke JNK1 / 2 inhibitor [1].

Ọmụmụ ihe na vivo:isovitexin (50 na 100 mg / kg, IP) kpatara mgbanwe akụkọ ihe mere eme na-adịchaghị njọ na ngalaba ngụgụ ma belata ọnụ ọgụgụ sel mkpali na ụmụ oke na-ebute LPS.Heteroeosinophils (50 na 100 mg / kg, IP) belatara TNF site-α na mmepụta IL-6, mmepụta ROS, MPO na ọdịnaya MDA, ụba SOD na GSH, ma gbochie LPS na-ebute mbufụt na nrụgide oxidative na LPS mere Ali ụmụ oke.Na igbochi okwu protein nke iNOS na COX-2 [1] nke ọma.Isovitexin (25,50, 100 mg / kg) belatara ọnụego nlanarị nke LPS / D-gal butere mmerụ ahụ imeju na ụmụ oke n'ụzọ dabere na dose.Isovitexin gbochiri NF-κ B wee na-achịkwa LPS / D-gal butere Nrf2 na HO-1 na ụmụ oke [2].

Nnwale cell:Ekpebiri ike ndụ cell site na nyocha MTT.A na-etinye mkpụrụ ndụ 264.7 raw na efere 96 nke ọma (1) × 104 sel / nke ọma) yana ntinye dị iche iche nke isovitexin (ntụgharị ikpeazụ: 0-200) μ G / ml) na LPS (2 μ G / ml) maka awa 24.Na mgbakwunye, a na-eji IV (25 ma ọ bụ 50 μG / ml) mee ka sel ahụ sie ike maka elekere 1, wee tinye H 2O 2 (300) μM) mgbe awa 24 gachara, a na-agbakwunye MTT (5 mg / ml). mkpụrụ ndụ wee tinye ya maka awa 4 [1].

Nnwale anụmanụ:oke [1] iji guzobe ụdị Ali, a na-ekewa ụmụ oke na-enweghị usoro n'ime otu isii: njikwa (saline), naanị isovitexin (100 mg / kg, agbazere na 0.5% DMSO), naanị LPS (0.5 mg / kg, gbazere na saline). ), LPS (0.5 mg / kg) + isovitexin (50 ma ọ bụ 100 mg / kg) na LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, gbazere na saline).A na-enye isovitexin ma ọ bụ DEX (5 mg / kg) isovitexin.Mgbe ekpughere isovitexin ma ọ bụ DEX maka awa 1, a na-eji ether kụnwụrụ ụmụ oke ma nyekwa LPS intranasally (in) iji kpata mmerụ ahụ nku ume.Achụpụrụ anụmanụ ndị ahụ awa iri na abụọ ka nchịkwa LPS gachara.Ya mere, a na-egbute mmiri mmiri bronchoalveolar lavage (BALF) na ngụgụ anụ ahụ iji tụọ ọkwa cytokine;ROS ọgbọ;SOD, GSH, MDA na MPO omume;Na okwu nke COX-2, iNOS, HO-1 na Nrf2 proteins [1].