Ngwaahịa

Nkwadebe ngwọta ntụaka:Were ọnụ ọgụgụ kwesịrị ekwesị nke Ruscogenin ntụ ntụ ntụ ntụ, tụọ ya nke ọma, ma gbakwunye methanol iji mee ka ọ nwere 50% kwa 1ml μG ngwọta. Nkwadebe nke usoro mgbagwoju anya nke ọma tụọ 1 ml, 2 ml, 3 ml, 4 ml, 5 ml na 6. ml nke ntụ ntụ ntụ ntụ, tinye ha na conical flask nke nwere ihe nkwụsị n'otu n'otu, ma kpochapu ihe mgbaze n'ime mmiri ịsa ahụ.Tinye 10ml perchloric acid nke ọma, gbanye ya nke ọma, debe ya na mmiri ọkụ maka nkeji 15, wepụ ya, mee ka ọ dị jụụ na mmiri ice, were reagent kwekọrọ dị ka oghere, tụọ nnabata ahụ na ebili mmiri nke 397nm dị ka ultraviolet na-ahụ anya spectrophotometry. Ihe mgbakwunye VA), were nnabata ahụ dị ka ordinate na itinye uche dị ka abscissa, wee see usoro ọkọlọtọ.

Nkwadebe ngwọta nnwale:Were ihe dị ka 3G ntụ ntụ nke ngwaahịa ahụ, tụọ ya nke ọma, tinye ya na flask conical nke nwere ihe nkwụsịtụ, tinye nke ọma 50ml nke methanol, tụọ ya, kpoo na reflux maka awa 2, mee ka ọ dị jụụ, tụọ ya, mee ka ọ dị arọ furu efu. ya na methanol, fesa ya nke ọma ma kpochaa ya.Gwakọta nke ọma 25ml nke nzacha na-aga n'ihu, tinye ya n'ime efere, weghachite ihe mgbaze ahụ ka ọ kpọọ nkụ, tinye 10ml mmiri ka ọ gbazee nke fọdụrụnụ, gbanye ya na mmiri, gbanye ya na n-butanol ugboro 5, 10ml oge ọ bụla, jikọta n. -butanol ngwọta, sachaa ya ugboro abụọ na amonia ngwọta, 5ml oge ọ bụla, tụfuo ngwọta amonia, na kpochapu n-butanol ngwọta na nkụ.Gbanyụọ ihe fọdụrụ na methanol 80% ma bufee ya na flask volumetric 50ml, tinye 80% methanol na nha ma maa jijiji nke ọma.

Mkpebi usoro n'ụzọ ziri ezi tụọ 2 ~ 5ml nke ule ngwọta, tinye ya na 10ml plugged akọrọ ule tube, dị ka usoro n'okpuru nkwadebe nke ọkọlọtọ usoro, tụọ absorbance dị ka iwu si "volating ihe mgbaze na mmiri bat", gụpụta ọnụọgụ nke ruscoegenin na ngwọta ule site na ọkọlọtọ ọkọlọtọ wee gbakọọ ya.

Ngụkọta saponins nke Ophiopogon japonicus agaghị abụ ihe na-erughị 0.12% dabere na Ruscogenin (C27H42O4).

Ọnọdụ chromatographic: (maka naanị ntụnye aka)