Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
Ozi dị mkpa
Salvianolic acid B bụ condensation nke molekul atọ nke Danshensu na otu molekul nke caffeic acid.Ọ bụ otu n'ime acid salvianolic a na-amụ karịa.Ọ nwere mmetụta ọgwụ dị mkpa na obi, ụbụrụ, imeju, akụrụ na akụkụ ndị ọzọ.Ngwaahịa a nwere mmetụta nke ịkwalite mgbasa ọbara na iwepu stasis ọbara, na-adọkpụ meridians na ịgbalite ihe nkwekọrịta.A na-ejikarị agwọ ọrịa strok nke ischemic nke ọbara stasis na-egbochi meridians, dị ka nhụjuanya nke ọkara ahụ na aka, adịghị ike, mgbu nkwekọrịta, ọdịda moto, mgbapụ ọnụ na anya, wdg.
Aha aha:salvianolic acid B, salvianolic acid B, salvianolic acid B
Aha Bekee:salvianolic acid B
Usoro ihe omimi:c36h30o16
Arọ molikula:718.62
Nọmba CAS:115939-25-8
Ụzọ nchọpụta:HPLC ≥ 98%
Nkọwapụta:10mg, 20mg, 100mg, 500mg, 1g (nwere ike ngwugwu dị ka ahịa chọrọ)
Ọrụ na ojiji:A na-eji ngwaahịa a maka ikpebi ọdịnaya.
Njirimara anụ ahụ na kemịkal
Njirimara:ngwaahịa bụ quasi ọcha ntụ ntụ.
Ụtọ ahụ dị ntakịrị ilu na astringent, nke nwere mmiri na-ebute ihe onwunwe.Soluble na mmiri, ethanol na methanol.
A na-emepụta Salvianolic acid B site na condensation nke molekul 3 nke salvianolic acid na 1 molecule nke caffeic acid.Ọ nwere otu carboxyl abụọ ma dị n'ụdị nnu dị iche iche (K +, Ca2 +, Na +, NH4 +, wdg).N'ime usoro Decoction na ntinye uche, a na-agbanye obere akụkụ nke salvianolic acid B na-acha odo odo oxalic acid na salvianolic acid, na akụkụ nke salvianolic acid B na-aghọ rosmarinic acid n'okpuru ọnọdụ acidic;Salvianolic acid A na C nwere ike ịbụ tautomeric na ngwọta.
Nkọwapụta
5%, 10%, 50%,
Usoro mmịpụta
A na-egweri Radix Salviae Miltiorrhizae, tinye ya n'ime tank nke mmịpụta, tinye ya na 0.01mol / l hydrochloric acid ugboro 8 n'otu abalị, wee tinye ya na okpukpu 14 karịa mmiri.A na-eji kọlụm resin macroporous AB-8 na-asachapụ ihe ngwọta ahụ e wepụrụ agbasa.Mbụ, elute na 0.01mol/l hydrochloric acid wepụ adịghị adsorbed adịghị ọcha, na mgbe ahụ elu na 25% ethanol iji wepụ nke ukwuu pola adịghị ọcha.N'ikpeazụ, tinye uche na 40% ethanol eluent n'okpuru nrụgide dị ntakịrị iji nwetaghachi ethanol na ifriizi-akọrọ iji nweta mkpokọta Salvia miltiorrhiza phenolic acid na ịdị ọcha karịa 80%.
Chọpụta
Were 1g nke ngwaahịa ahụ, gwerie ya, gbakwunye 5ml nke ethanol, kpalie nke ọma, nyo, were ntakịrị mmiri nzacha, tinye ya na mpempe akwụkwọ nzacha, kpoo ya, hụ ya n'okpuru oriọna ultraviolet (365nm), gosi blue- fluorescence isi awọ, kpọgidere akwụkwọ nzacha n'ime karama ngwọta amonia gbanyere mkpọrọgwụ (anaghị akpọtụrụ elu mmiri), wepụ ya mgbe nkeji iri abụọ gachara, lelee ya n'okpuru oriọna ultraviolet (365nm), na-egosi fluorescence na-acha anụnụ anụnụ.
Acidity:were ngwọta mmiri n'okpuru ihe doro anya, na pH uru ga-abụ 2.0 ~ 4.0 (ihe mgbakwunye nke Chinese Pharmacopoeia 1977 Edition).
Mkpebi ọdịnaya
Ekpebiri ya site na chromatography mmiri na-arụ ọrụ dị elu (Ihe Okike VI D, Mpịakọta I, Chinese Pharmacopoeia, 2000 EDITION).
A na-eji Octadecyl silane bonded silica gel mee ihe dị ka ihe ndochi na ọnọdụ chromatographic na ule ntinye usoro;Methanol acetonitrile formic acid mmiri (30:10:1:59) bụ usoro mkpanaka;Ogologo ebili mmiri a chọpụtara bụ 286 nm.Ọnụọgụ nke efere usoro ihe omume gbakọrọ dabere na salvianolic acid B elu agaghị abụ ihe na-erughị 2000.
Nkwadebe ntụaka n'ụzọ ziri ezi tụọ ego kwesịrị ekwesị nke salvianolic acid ngwọta ntụaka B ma tinye mmiri ka ọ nwee 10% kwa 1ml μG ngwọta.
Nkwadebe nke ngwọta ule were ihe dị ka 0.2g nke ngwaahịa ahụ, tụọ ya nke ọma, tinye ya na karama nha 50ml, gbakwunye methanol kwesịrị ekwesị, sonicate maka nkeji 20, mee ka ọ dị jụụ, tinye mmiri n'ogo, gbanye ya nke ọma, iyo. ya, tụọ nke ọma 1ml nke nzacha na-aga n'ihu, tinye ya n'ime karama nha 25ml, tinye mmiri na ọnụ ọgụgụ, maa jijiji nke ọma.
Usoro mkpebi siri ike na-amịkọrọ 20% nke ngwọta nchịkwa yana 20% nke ngwọta ule μl.Tinye ya n'ime chromatograph mmiri mmiri maka mkpebi siri ike.
Ịrụ ọgwụ ọgwụ
Salvianolic acid B bụ condensation nke molekul atọ nke Danshensu na otu molekul nke caffeic acid.Ọ bụ otu n'ime acid salvianolic a na-amụ karịa.Ọ nwere mmetụta ọgwụ dị mkpa na obi, ụbụrụ, imeju, akụrụ na akụkụ ndị ọzọ.
Antioxidant
Salvianolic acid B nwere mmetụta antioxidant siri ike.Nnwale na vivo na in vitro na-egosi na salvianolic acid B nwere ike ịbọ ọbọ oxygen free radicals na igbochi lipid peroxidation.Ike omume ya dị elu karịa nke vitamin C, vitamin E na mannitol.Ọ bụ otu n'ime ngwaahịa sitere n'okike nke nwere mmetụta antioxidant kachasị ike, ọmụmụ ọgwụ na-egosi na salvianolic acid maka ịgba ọgwụ nwere mmetụta antioxidant doro anya, na-egbochi nchịkọta platelet na thrombosis, ma nwee ike ịgbatị oge ndụ anụmanụ n'okpuru hypoxia.Nsonaazụ gosiri na salvianolic acid maka ịgba ogwu (60 ~ 15mg / kg) nwere ike imeziwanye ụkọ akwara ozi na oke nwere mmerụ ahụ nke ischemia-reperfusion, melite nsogbu omume ma belata oke nke infarction ụbụrụ.Enwere nnukwu ọdịiche dị n'etiti oke na nke ọkara (60 na 30mg / kg);Salvianolic acid maka ịgba ọgwụ nwere ike imeziwanye mmebi akwara ozi nke FeCl3 butere cerebral ischemia na oke na 1, 2 na 24 awa mgbe nchịkwa gasịrị, nke a na-egosipụta na mmụba nke nsogbu omume na mbelata mpaghara infarction ụbụrụ;Salvianolic acid 40 mg / n'arọ maka ịgba ọgwụ mgbochi gbochiri nchịkọta platelet oke bekee nke ADP, arachidonic acid na collagen kpatara, na ọnụego mgbochi bụ 81.5%, 76.7% na 68.9% n'otu n'otu.Salvianolic acid 60 na 30mg / n'arọ maka ịgba ọgwụ mgbochi egbochiri thrombosis na oke;Salvianolic acid 60 na 30mg / n'arọ maka injection gbatịkwuru ogologo oge ndụ ụmụ oke n'okpuru hypoxia.
Ngwa Clinical
Ngwaahịa a nwere mmetụta nke ịkwalite mgbasa ọbara na iwepu stasis ọbara, na-adọkpụ meridians na ịgbalite ihe nkwekọrịta.A na-ejikarị agwọ ọrịa strok nke ischemic nke ọbara stasis na-egbochi meridians, dị ka nhụjuanya nke ọkara ahụ na aka, adịghị ike, mgbu nkwekọrịta, ọdịda moto, mgbapụ ọnụ na anya, wdg.
Ụlọ ahịa
N'ebe dị jụụ na nkụ.
Oge nkwado
Afọ abụọ.
Usoro nchekwa
2-8 Celsius C, echekwara n'ebe dị jụụ na nkụ ma pụọ na ìhè.
Ihe ndị chọrọ nlebara anya
Ekwesịrị ịchekwa ngwaahịa ahụ na obere okpomọkụ.Ọ bụrụ na ekpughere ya na ikuku ruo ogologo oge, ọdịnaya ga-ebelata.
