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Target: GABA receptor, mTOR, PI3K, Akt [1] [2]

In vitro study: low dose 41 μ Jujuboside A of M (about 0.05 g / L) induced GABA (a) receptors during 24 and 72 h treatment α 1， α 5， β 2 subunit mRNA increased significantly. High dose 82 μ M (about 0.1 g / L) Jujuboside A significantly increased GABA (a) receptors at 24 h α 1， α 5 subunit mRNA levels and decreased β 2 subunit mRNA level and decreased GABA (a) receptor subunit α 1， β 2 mRNA expression was treated at 72 h [1]. Jujuboside A pretreatment can reverse the decrease of cell viability and better induce ISO induced H9c2 cell injury. Jujuboside A can accelerate the phosphorylation of PI3K, Akt and mTOR. Jujuboside A can significantly reduce the proportion of microtubule associated protein LC3-II / I in H9c2 cells [2]

In vivo study: during the day (9:00-15:00), jujubosides significantly increased total sleep and rapid eye movement (REM) sleep, but had no significant effect on non REM (NREM) sleep. At night (21:00-3:00), jujubosides significantly increased total sleep and NREM sleep, especially mild sleep, but had no significant effect on REM sleep and slow wave sleep (SWS) [3]. Intraventricular treatment with jjuboside a was significantly alleviated by a through Y-maze, active avoidance and Morris water maze measurements β 1-42 induced learning and memory impairment in mice. Intracerebroventricular injection of Jujuboside A decreased hippocampal a β 1-42 levels significantly inhibited the activities of acetylcholinesterase (AChE) and no, and decreased the increased content of malondialdehyde (MDA) in hippocampus and cerebral cortex of mice injected into lateral ventricle β 1-42[4]。

Cell experiment: after 7 days of culture in vitro, the cells were continuously exposed for 24 hours or 72 hours, and were not exposed to the medium containing Jujuboside A or diazepam or both. Vector was added to the control group; Diazepam group added 10 μ M diazepam; Jujuboside A82 μ M (about 0.1g / L) and 41 μ M (about 0.05 g / L) was added to jua-h (high dose Jujuboside A) and jua-l (low dose Jujuboside A) groups, respectively. Total RNA was isolated from cells for further analysis [1].

Animal experiment: mice: a was injected through ICV β 1-42 induced cognitive impairment in mice. Then, mice were injected with Jujuboside A (0.02 and 0.2 mg / kg) intraventricular (ICV) for 5 days. Y maze, active avoidance and Morris water maze tests were performed on mice [1].