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Descriptio:
prim-o-glucosylcimifugin potentes effectus anti-inflammatorii habet.Expressio iNOS et COX-2 inhiberi potest ordinando JAK2 / STAT3 signo transd

Genera pertinentia:
> > > > Synthasis oxydi nitrici signans iter > > immunitatem et inflammationem
Investigationes campi > ​​> inflammatio / immunitas
Natural Products >> alii

Scopum:iNOS
COX-2

In Vitro Study:
prim-o-glucosylglycine (POG) summa contentum chromone et unum principalium partium activarum in Fangfeng (RS).Prim-o-glucosylglycines partes inflammatorias in crudis 264,7 macrophages anti-inflammationem agit, inhibendo JAK2 / STAT3 transductionem insignem et inhibitionem expressionis iNOS et COX-2.Cytotoxicitas prim-o-glucosidis in LPS reducitur rudis 264,7 macrophages mensurata.Utere LPS (1 μ G / ml) et augendae collectiones primitivorum o-glucosylglycini (15, 50 et 100 μ G/ml) per 24 horas, et cellae viability a CCK-8 examinata fuit.Comparatus cum DMSO cellulis tractatis (imperium), 24 horis et nuditate ad 15-100 μ Post g/ml prim-o-glucosidum, cellula viability cellularum non signanter affectus est.Ad investigandum effectum anti-inflammatorii primi o-glucosylglycini, utrum primum o-glucosylglycinum afficere possit, nulla synthesis examinata est in LPS cellulas crudas 264,7.Utere LPS (1 μ G/ml) et variis concentratione primitivorum o-glucosylglycini (15, 50 et 100 μ G/ml) pro 24 horis.Contentio non mensuratur in cultura supernatantis per reactionem Griss.Concentratio nullius in cultura supernatantis signanter aucta cum LPS detectione, et prim-o-glucoside insigniter inhibita LPS inducebat nullam productionem in modo dependens dependens [1].
In Vivo Study
lavacrum bronchoalveolaris fluidum (BALF) colligebatur 7 horis post lipopolysaccharidum (LPS) administrationem, et cytokinum in BALF mensuratum ab ELISA.Comparatus cum globo regimine, TNF in BALF- α， IL-1 β et IL-6 gradus insigniter auctus.Praetractatio autem cum primo-o-glucosylglycine (2.5, 5 vel 10 mg / kg) signanter ordinatur TNF- α， IL-1 β Et IL-6 in modo dependens (P < 0.05, P < 0.05, 0.01 ) [1].

Cell Experimentum
cellula computationis Ornamentum (CCK-8) adhibitum est ad determinandam intentionem cytotoxicam primi o-glucosylglycini.In summa, cruda 264.7 cellulae 1 per bene tractatae sunt densitas 104 cellularum inoculata erat in 96 puteis et incubatis pernoctare.Tum usus 1 μ Cellae g/ml LPS excitatae sunt et variis concentratione primitivae glucosylglycini (15, 50 et 100 µ g/mL; exprimuntur, Princeton, NJ, USA) vel DMSO tractatum.Post incubationem ad 37 per 24 horas, solutio CCK-8 singulis bene addita est, et per aliam horam incubata.Absorbance was metiri in 450 nm utens microplato lectore [1].

Animal Experimentum
mures [1] BALB / C mures masculi, VIII hebdomades quinque, appendentes circiter 18 ad 20g.Mures passim in 5 circulos divisi sunt: ​​coetus control;catervae LPS;LPS + primi-o-glucosylglycinum (2.5, 5 vel 10mg / kg pondus corporis).Primi-o-glucosylglycine intraperitonealiter administrata est.Post horam 1 , mures in LPS globi et LPS + globi primi-o-glucosylglyci dati sunt 50 mg / L intranasale (in) (200 mg / L) μ LLPs ad laesionem acutam pulmonis inducendam.Imperium mures datae sunt L% intranasales sine LPS μ LPBS
[1]

Relatio:
[1].Zhou J, et al.Prim-O-glucosylcimifugin Attenuat Responsio Inflammatoriae Lipopolysaccharideinduced in RAW 264,7 Macrophages.Pharmacogn Mag.2017 Iul-Sep;
[2].Chen N, et al.Primi-O-glucosylcimifugin attenuat lipopolysaccharide-inducta injuria pulmonis acuti in muribus.Int Immunopharmacol.2013 Jun; 16(2):139-47.