Glycyrrhizin, Liquiritin;Liquiritoside;Likviritin;Liquiritoside Cas No.551-15-5
Kupu Whakataki Poto
Ko Glycyrrhizin, e mohiotia ana ko Liquiritin.Ko te Licorice he tipu o Glycyrrhiza i Leguminosae.Ko ona pakiaka me ona kakau he otaota Hainamana noa.
Ka tohatohahia te rongoa ki te Rawhiti o Haina, Xinjiang, Yunnan, Inner Mongolia, Anhui me etahi atu waahi.Ka whakarārangihia e Shennong materia medica classic te reanga teitei, e kii ana "ko tenei tarutaru te kingi o nga rongoa katoa, a he tokoiti te hunga karekau e whakamahi".Ko te Licorice he waahanga uaua, ko te nuinga ko te triterpenoids, flavonoids me te coumarins.Ko nga flavonoids he momo waahanga koiora i puta mai i te tangohanga raihana.Ko ona wahanga matū rongoa ko te glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, me etahi atu. I nga tau kua pahure ake nei, kua puta nga korero mo te whakaheke kore utu, antioxidant, anti-cancer me nga hua anti mutagenic o te flavonoids licorice i te kainga me waho.
Te matūNahau:4H-1-Benzopyran-4-one, 2- [4-(β-D-glucopyranosyloxy) phenyl]-2, 3-dihydro-7-hydroxy-, (S)
PtinanaPtika:Monohydrate (waiwai waimeha wai ranei), ira whakarewa: 212 ~ 213 ° ℃.
Mahi Rongoa
Paitini: Karekau
Te tauhohenga kino: kaore e mohiotia
Te puna whakauru: legume Glycyrrhiza glabra L. pakiaka, Glycyrrhiza uralensis Fisch Root.
Tangohanga o Glycyrrhizin
Pretreatment of Licorice Raw Materials
He tino uaua te hanganga matū o te rauemi mata raihana.Kia pai ake ai te painga o te wehenga, whakaitihia te poke o nga poke i runga i te chromatographic tīwae whakarite, me te whakapai ake i te ihirangi o te glycyrrhizin i roto i te werohanga rauemi mata, i whakamahia te tikanga tangohanga ki te rongoa i te rauemi mata.Paunahia te tarutaru 4G me te toenga hiko ka maka ki roto i te ipurau.Me ine tika te 100ml wai matutu me te ine ine ka riringi ki roto i te ipurau mo te memeha.Ultrasonic mo te 15 meneti, me te whakaoho tonu me te rakau karaihe hei whakatere i te whakakore.Katahi ka kuhua te ipurau ki roto i te 90 ℃ pati whakamahana ka whakamahana mo te 2 haora, ka whakamahana mo te tātari.Whai muri i te taapiri i te filtrate ki te whakarewa n-butanol me te tu mo etahi meneti, ka memeha te nuinga o nga glycosides i roto i te whakarewa n-butanol, katahi ka whakahaere i te tangohanga tuarua, ka tango i te iti o nga glycosides e toe ana i roto i te wai, ka mutu ka whakakotahi me te aro ki te n- te otinga butanol i whiwhi mai i te tangohanga tuarua mo te chromatography me te pure.
Te purenga o Glycyrrhizin Na Chromatography
Tangohia te 10 ml o te hua kua tangohia mai i runga ake nei hei rauemi mata e manawapa, timata te papu, whakatauhia te tere rere ki te 25 ml / min, ka kawe mai i te rauemi mata ki roto i te 500 mm ma te wahanga pūkoro (methanol: wai = 1:4) × I roto i te 40 mm tīwae takahanga, kohia te hautanga o te tarutaru glucoside hua i runga i te ahuatanga o te tihi: ko te hautanga o te 1 h tuatahi ka kohia hei hautanga o mua i te poke, katahi ka huri i te rere.Hei tauira, horoia te pou me te ranunga o te 50% methanol me te wai, honoa te hua ia 20 meneti, katahi ka aro ki ia pounamu hua ki te whakaetonga hurihuri, ka tango i te 20 µ L mo te tātari chromatographic HPLC, Kia kore ra e kitea te whainga.Ko nga ahuatanga o te rapunga o te HPLC e whai ake nei: te wahanga pūkoro: methanol: wai = 3.5:6.5;Wāhanga noho: silica gel waro 18;Tīwae Chromatographic: 450 mm × 4.6 mm ； Rere Rere: 1 ml / min;Te roangaru rapu: 254nm.Ko te ihirangi o te glycyrrhizin i roto i te pounamu tuarua ko te mea teitei rawa atu i roto i nga hua ka riro ia 20 min
Te purenga o Glycyrrhizin Na Rechromatography
I te mea ko te ihirangi o te glycyrrhizin i muri i te purenga chromatographic tuatahi kaore i te teitei, ka tohua te tikanga ano.Tangohia 10 ml o te hua purea i runga ake rite te rauemi mata tūtatari, te auau rere ko te 25 ml / min, ka kawea te pounamu tuarua o te hua ki te 500 mm i te wahanga pūkoro (methanol: wai = 2:5) × I roto i te 20 mm. } tīwae chromatographic, kohia te distillate o te tarutaru glycoside hua i runga i te ahua o te tihi: hono te hua ia 4 min, ka kukū ia ipu o te hua ki te whakaetonga hurihuri, me te whakamahi i te taua Unuhia kitea i runga ake mo te HPLC chromatographic tātaritanga tae noa ki kahore he ūnga .I muri i te tātaritanga, i kitea ko te ihirangi o te glycyrrhizin i roto i te ono o nga pounamu ko te mea nui rawa atu i roto i nga hua i riro ia 4 meneti, i roto i te waa pupuri ko te 5.898 min hei te tihi o te whaainga, a ko te ihirangi i tae atu ki te 40% na te tikanga whakatikatika rohe. .
Post Treatment o Hua
Ko te hua kua kohia ka whakamarohia i raro i te pehanga iti i runga i te whakaetonga hurihuri i te 70 ℃.I muri i te tino whakaeto o te whakarewa, whakarewahia te hua totoka i runga i te ipu porotaka o raro me te iti o te mewaro, ka whakamaarama i roto i te ipuipu i te pāmahana rūma tae noa ki te puta mai o nga tioata ma.
