Hua

Typhonine glycoside

Ko Puhuang te hae maroke o Typha angustifolia L., Typha Orientalis Presl, tipu rite ranei o te Typhaceae.Ko te ihirangi o te isorhamnetin-3-o-neohesperidin i te Hainamana Pharmacopoeia (2005 Edition) ka whakatauhia e HPLC.Ko nga tikanga matua mo te whakatau i te isorhamnetin-3-o-neohesperidin i Typhae me ona whakaritenga ko

HPLC.I raro i te whakatau ihirangi o Typha i te Hainamana Pharmacopoeia (2005 Edition):

Nga tikanga Chromatographic me te whakamatautau mo te whakamahi i te punaha: octadecyl silane here silica gel hei whakakii;Ko te wai acetonitrile (15:85) i whakamahia hei wahanga pūkoro;Ko te roangaru kitea he 254 nm.Te pāmahana tīwae 40 ℃.Ko te maha o nga pereti ariā kaua e iti iho i te 1000 kia rite ki te tihi o te isorhamnetin-3-o-neohesperidin.

Te takanga o te otinga whakamatautau: tangohia kia 0.5 karamu o tenei hua, pauna tika, hoatu ki roto i te pounamu ine 50ml, taapirihia te 45ml methanol, maimoatanga ultrasonic (mana 250W, auau 20KHz) mo te 30 meneti, whakamatao, taapirihia te methanol ki te tauine, ruru pai, tātarihia, ka tango i te filtrate tonu

Te whakatau i te isorhamnetin-3-o-neohesperidin i Puhuang me ana whakaritenga na HPLC.Ko te punaha waahanga pūkoro he wai acetonitrile:

① Hp1050 mahi teitei wai chromatograph;Chromatographic tīwae C18 (5) μ m,4.6mm × 250mm) Dalian Institute of Chemical Physics, Chinese Academy of Sciences);Wāhanga pūkoro: wai acetonitrile (15:85);Te roangaru rapu: 254nm;Te pāmahana kōtuhi tīwae.

② Ko te chromatograph wai mahi teitei ko te punaha hp1050 a Hewlett Packard Company i te United States;Ko te karaka here o te pou Chromatographic 10 C18 (3.9mm) × 300mm； Wāhanga pūkoro: wai acetonitrile (80:20);roangaru kitea 254 nm, hōkaiipurangi 4 nm;roangaru tohutoro 550 nm, hōkaiipurangi 100 nm;whakaheke 8.

Fu Daxu et al.I whakatauhia nga ihirangi o te typhoniflorin me te isorhamnetin-3-o-neohesperidin i roto i te tangohanga flavone katoa o Typha na HPLC me te 1100 chromatograph.Ko te tīwae chromatographic he eclipse XdB C18 (250mm × 4.6mm，5 μm) ； Wāhanga pūkoro: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15:78:7; 15min: 23:70:7);Ko te roangaru kitea he 248 nm.Te whakarite i te katoa o te tangohanga flavonoids o Typha: 50g o Typha i tangohia me te 60% ethanol (500ml) × 2), 1H i ia wa, ka whakakoihia te tangohanga ki te 40 ℃, ka rewa te tangohanga ki te wai 600ml me te centrifuged.Ko te toenga ka rewa ki te 300ml wai me te centrifuge.Whakakotahitia te wairewa centrifugal, whakatikahia te rōrahi ki te 1000ml me te wai, ka toha ki runga i te pou o te kapia macroporous (150ml kapia macroporous maroke te taumaha 75g) me te 1.5 nga wa te nui o te tarukino masivesivá, elute ki te 600ml o te wai me te 450ml o te 20% te waihā hei tango. nga poke, katahi ka whakahiatotia ki te 600ml o te 50% te waihano, ka whiwhi te eluent ma te whakamaroke korehau i te 40 ℃.

Yang Yonghua et al.I whakamahia te hiko o te capillary teitei me te chromatography wai mahi teitei hei whakatau i nga ihirangi o te isorhamnetin 232o2, te neohesperidin me te typhoside i Puhuang.Hpce-3d taputapu hiko hiko o te kaporeihana teitei (Hewlett Packard, USA), te pou hiko kiripaka 50 μm × 56cm (HP), lc-6a chromatograph wai mahi teitei (Kamupene Shimadzu o Hapani), pou C18 5mm × 200mm, rahi matūriki 5 μ M (Kamupene Dalian Yite).Tikanga HPCE: parepare 0.02mol/l borax-0.05mol/l 10% acetonitrile o konutai dodecyl pūkawa ngāwha (SDS), ngaohiko 20kV, pāmahana tīwae 30 ℃, rapunga roangaru 270nm, rōrahi werohanga 30KPa · s.Nga tikanga HPLC: he wai acetonitrile te wahanga pūkoro (15:85), he 25 ℃ te pāmahana o te pou, a ko te roangaru rapu ko te 254 nm.

Matū Tohutoro Typhaneoside

[ingoa]typhonioside [1]

[CAS No.]104472-68-6

[tikanga rapu]HPLC ≥ 98%

[Whakaahuatanga]20mg 50mg 100mg 500mg 1g (ka taea te kohikohi kia rite ki nga whakaritenga a te kaihoko)

[āhuatanga]paura amorphous