Glycyrrhizin, Liquiritin;Liquiritoside;Likviritin;Liquiritoside Cas No.551-15-5
Mawu Oyamba Mwachidule
Glycyrrhizin, yemwe amadziwikanso kuti Liquiritin.Licorice ndi chomera cha Glycyrrhiza ku Leguminosae.Mizu yake ndi zimayambira ndi zitsamba zofala zaku China.
Mankhwala amafalitsidwa kwambiri kumpoto chakum'mawa kwa China, Xinjiang, Yunnan, Inner Mongolia, Anhui ndi malo ena.Shennong materia medica classic imatchula kuti ndi kalasi yapamwamba, ponena kuti "udzu uwu ndi mfumu ya mankhwala onse, ndipo ndi ochepa omwe saugwiritsa ntchito".Licorice ali ndi zigawo zovuta, makamaka triterpenoids, flavonoids ndi coumarins.Flavonoids ndi mtundu wa zigawo za bioactive zomwe zimachokera ku licorice extract.Mankhwala ake a mankhwala a mankhwala makamaka amaphatikizapo glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, ndi zina zotero.
ChemicalName:4H-1-Benzopyran-4-one, 2- [4-(β-D-glucopyranosyloxy) phenyl] -2, 3-dihydro-7-hydroxy-, (S)
PhysicalPnyumba:Monohydrate (kuchepetsa ethanol kapena madzi), malo osungunuka: 212 ~ 213 ° ℃.
Pharmacological Action
Kaizoni: Palibe
Zoyipa: zosadziwika
Zopangira: nyemba Glycyrrhiza glabra L. muzu, Glycyrrhiza uralensis Fisch Root.
Kutulutsa kwa Glycyrrhizin
Kukonzekera Kwazinthu Zopangira Licorice
Kapangidwe kake ka licorice yaiwisi ndizovuta kwambiri.Kuti mupeze zotsatira zabwino zolekanitsa, kuchepetsa kuipitsidwa kwa zinyalala pamzati wokonzekera chromatographic, ndikuwongolera zomwe zili mu glycyrrhizin muzopangira jekeseni, njira yochotsera idagwiritsidwa ntchito popangira zinthu zopangira.Yesani udzu wa 4G ndi banki yamagetsi ndikuyiyika mu beaker.Yesani bwino 100ml madzi osungunuka ndi silinda yoyezera ndikutsanulira mu beaker kuti asungunuke.Akupanga kwa mphindi 15, ndipo nthawi zonse akuyambitsa ndi galasi ndodo kuti imathandizira kuvunda.Kenako ikani beaker mu bafa yotentha ya 90 ℃ ndikutenthetsa kwa maola awiri, kenako itenthetseni kuti isefe.Pambuyo powonjezera zosefera mu n-butanol zosungunulira ndikuyimirira kwa mphindi zingapo, ma glycosides ambiri amasungunuka mu n-butanol zosungunulira, kenako amachotsa yachiwiri, kuchotsa pang'ono glycosides otsala m'madzi, ndipo pomaliza amaphatikiza ndi kuyika n- yankho la butanol lomwe limapezedwa ndi kutulutsa kwachiwiri kwa chromatography ndi kuyeretsa.
Kuyeretsedwa Kwa Glycyrrhizin Ndi Chromatography
Tengani 10 ml ya zomwe zatulutsidwa pamwambapa ngati zopangira zotsalira, yambani mpope, ikani kuthamanga kwa 25 ml / min, ndikubweretsa zopangirazo mu 500 mm ndi gawo la mafoni (methanol: madzi = 1: 4) × Mzere wokonzekera 40 mm, sonkhanitsani kachigawo kakang'ono ka udzu wa glucoside malinga ndi momwe zinthu zilili: gawo loyamba la 1 h limasonkhanitsidwa pamodzi ngati gawo losadetsedwa, ndiyeno sinthani kutuluka.Mwachitsanzo, sambani mzati ndi chisakanizo cha 50% methanol ndi madzi, gwirizanitsani mankhwalawa mphindi 20 zilizonse, ndiyeno sungani botolo lililonse lazinthu ndi nthunzi yozungulira, ndikutenga 20 µ L pakuwunika kwa HPLC chromatographic, Mpaka palibe chandamale chomwe chadziwika.Zomwe anapeza za HPLC zinali motere: gawo la mafoni: methanol: madzi = 3.5: 6.5;Gawo loyima: silika gel osakaniza mpweya 18;Mzere wa Chromatographic: 450 mm × 4.6 mm; Kuthamanga: 1 ml / min;Kutalika kwa mawonekedwe: 254nm.Zomwe zili mu glycyrrhizin mu botolo lachiwiri ndizokwera kwambiri pakati pa zomwe zimalandiridwa mphindi 20 zilizonse
Kuyeretsedwa Kwa Glycyrrhizin Ndi Rechromatography
Popeza zomwe zili mu glycyrrhizin pambuyo pa kuyeretsedwa koyambirira kwa chromatographic sizokwera, njira yomweyo imasankhidwa.Tengani 10 ml ya mankhwala oyeretsedwa pamwamba monga zopangira standby, mlingo otaya ndi 25 ml / min, ndi kubweretsa botolo lachiwiri mankhwala mu 500 mm ndi mafoni gawo (methanol: madzi = 2:5) × Mu 20 mm. } chromatographic column, sonkhanitsani distillate wa udzu glycoside mankhwala malinga ndi mmene zinthu zilili pachimake: kulumikiza mankhwala 4 mphindi iliyonse, kenaka sungani botolo lililonse mankhwala ndi evaporation rotary, ndipo gwiritsani ntchito mzere wozindikira womwewo pamwamba pa HPLC chromatographic kusanthula mpaka palibe chandamale. .Pambuyo pakuwunika, zidapezeka kuti zomwe zili mu glycyrrhizin mu botolo lachisanu ndi chimodzi zinali zapamwamba kwambiri pakati pa zinthu zomwe zidalandilidwa mphindi 4 zilizonse, pomwe nthawi yosungiramo inali 5.898 min monga pachimake chandamale, ndipo zomwe zili m'botolo lachisanu ndi chimodzi zidafika pafupifupi 40% ndi njira yokhazikika yamalo. .
Post Treatment Of Products
Zomwe zasonkhanitsidwa zimasungunulidwa pansi pa kupanikizika kocheperako pa evaporator yozungulira pa 70 ℃.Chosungunuliracho chikatuluka nthunzi, sungunulani chinthu cholimba pa botolo lapansi lozungulira ndi methanol pang'ono, ndikuwunikira mu chubu choyesera kutentha kutentha mpaka makhiristo oyera granular awonekere [2].
