Zogulitsa

Zolinga:JNK1 jnk2 NF- ndi B

Maphunziro a in vitro:isovitexin imalepheretsa kuwonongeka kwa okosijeni kwa LPS poletsa kupanga kwa ROS ya intracellular, komanso kufooketsa mphamvu ya H2O2 pama cell.Muli LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) analibe cytotoxicity kwa yaiwisi 264.7 maselo, koma 200 μ G / ml isovitexin anasonyeza kwambiri cytotoxicity.Isovitexin (25,50) μ G / ml) yoletsa LPS inachititsa TNF- α, Kuwonjezeka kwa IL-6, iNOS ndi COX-2.Isovitexin (25,50) μ G / ml) inalepheretsanso I m'maselo aiwisi a 264.7 κ B α Phosphorylation ndi kuwonongeka, zomwe zimagwirizana ndi zotsatira za JNK1 / 2 inhibitor [1].

Maphunziro mu vivo:isovitexin (50 ndi 100 mg / kg, IP) idapangitsa kusintha kocheperako kwa histopathological m'magawo am'mapapo ndikuchepetsa kuchuluka kwa maselo otupa mu mbewa za LPS.Heteroeosinophils (50 ndi 100 mg / kg, IP) inachepetsa TNF ndi- α Ndipo IL-6 kupanga, kupanga ROS, MPO ndi MDA zokhutira, kuwonjezeka kwa SOD ndi GSH, ndipo kulepheretsa LPS kuchititsa kutupa ndi kupsyinjika kwa okosijeni mu LPS inachititsa Ali mbewa.Ndipo zimalepheretsa kufotokoza kwa mapuloteni a iNOS ndi COX-2 [1].Isovitexin (25,50, 100 mg / kg) idachepetsa kupulumuka kwa LPS / D-gal kuvulala kwachiwindi mu mbewa motengera mlingo.Isovitexin inaletsanso NF- κ B ndikuwongolera LPS / D-gal yochititsa Nrf2 ndi HO-1 mu mbewa [2].

Kuyesera kwa ma cell:Kuthekera kwa ma cell kumatsimikiziridwa ndi kuyesa kwa MTT.Yaiwisi 264,7 maselo katemera pa 96 bwino mbale (1) × 104 maselo / bwino) ndi woipa zosiyanasiyana isovitexin (omaliza ndende: 0-200) μ G / ml) ndi LPS (2 μ G / ml) kwa maola 24.Komanso, ntchito IV (25 kapena 50 μ G / ml) pretreated maselo kwa ola 1, ndiyeno H 2O 2 (300) anawonjezera μ M). Pambuyo maola 24, MTT (5 mg / ml) anawonjezera maselo ndiyeno incubated kwa maola 4 [1].

Kuyesera kwa zinyama:mbewa [1] kuti akhazikitse chitsanzo cha Ali, mbewa zinagawidwa mwachisawawa m'magulu a 6: kulamulira (saline), isovitexin yokha (100 mg / kg, kusungunuka mu 0.5% DMSO), LPS yokha (0.5 mg / kg, yosungunuka mu saline). ), LPS (0.5 mg / kg) + isovitexin (50 kapena 100 mg / kg) ndi LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, kusungunuka mu saline).Isovitexin kapena DEX (5 mg / kg) isovitexin idaperekedwa.Pambuyo pokhudzana ndi isovitexin kapena DEX kwa ola la 1, mbewa zinagwidwa ndi ether ndipo LPS inkaperekedwa intranasally (mu) kuti apangitse kuvulala kwamapapu.Nyamazo zidaloledwa maola 12 pambuyo pa kayendetsedwe ka LPS.Choncho, bronchoalveolar lavage fluid (BALF) ndi zitsanzo za minofu ya m'mapapo zinakololedwa kuti ziyese milingo ya cytokine;Mbadwo wa ROS;SOD, GSH, MDA ndi ntchito za MPO;Ndipo mawu a COX-2, iNOS, HO-1 ndi mapuloteni a Nrf2 [1].