Zogulitsa

Kufotokozera:
pachymic acid ndi triterpenoid yochokera ku P. cocos.Pachymic acid imalepheretsa njira zowonetsera za Akt ndi ERK.

Magulu ofananira:
kafukufuku > > khansa
Zachilengedwe > > terpenoids ndi glycosides

Zolinga:
Akt
ERK

Phunziro la In Vitro:
pachymic acid (PA) imatha kuletsa tumorigenesis ya khansa ya ndulu yokhudzana ndi njira zowonetsera za Akt ndi ERK.Chithandizo cha Pachymic acid (PA) chinalepheretsa kwambiri njira za Rho a, Akt ndi ERK m'maselo a khansa ya ndulu.Chithandizo cha Pachymic acid (PA) chingathe kuchepetsa PCNA, ICAM-1, RhoA, p-Akt ndi perk m'njira yodalira mlingo.Maola 12 mutalandira chithandizo, 10 μ G / ml Poria acid (PA) inaletsa kukula kwa maselo pamagulu a 30 μ G / ml kumachepetsanso kukula kwa maselo.Kukula kwa ma cell kunaletsedwa munthawi yake - komanso motengera mlingo.Pambuyo maola 48 mankhwala, ndende anali 10 μ g/ mL, 20 μ G/ml ndi 30 μ Pachymic acid (PA) pa g/ml linaletsa kukula kwa selo ndi za 25%, 40% ndi 70%.Pachymic acid (PA) imalepheretsanso kukula kwa maselo a khansa ya ndulu motengera nthawi komanso kutengera mlingo [1].

Mu Phunziro la Vivo:
pofuna kuyesa ntchito ya antitumor ya pachymic acid (PA) mu vivo, khansa ya m'mapapo ya munthu nci-h23 chotupa xenograft chitsanzo chinagwiritsidwa ntchito.Pachymic acid (PA) idalepheretsa kukula kwa chotupa pamlingo wa 30 ndi 60 mg / kg kwa masiku 21 poyerekeza ndi gulu lowongolera [2].

Kuyesa Maselo:
cell counting kit-8 (CCK-8) idagwiritsidwa ntchito poyesa anti proliferation effect ya pachymic acid (PA) pama cell a GBC-SD.Mwachidule, pambuyo mankhwala otchulidwa, kuwonjezera 10 aliyense dzenje μ Lcck-8 njira, ndipo pambuyo makulitsidwe mmodzi, kuyamwa anayesedwa pa 450 nm ntchito microplate owerenga [1].

Kuyesera kwa Zinyama:
mbewa [2] amagwiritsa ntchito mbewa zazikazi za athymic zazaka za 4-5 milungu.Maselo a nci-h23 omwe amakula mokulirapo (pa 100 μ 5 mu LPBs × 106) amabayidwa pang'onopang'ono mbali yakumanja ya mbewa iliyonse.Chotupa cha xenografts chinakula mpaka kukula kwa 100-200mm 3 ndipo mwachisawawa anapatsidwa magulu anayi osiyana a mankhwala (6 mbewa pagulu lililonse): (a) kuyendetsa galimoto (0.1% DMSO mu saline wamba);(b) Pachymic acid (PA) 10 mg / kg;(c) PA 30 mg/kg;(Dd) PA 60mg / kg. Mbewa zinapatsidwa jekeseni wa intraperitoneal (IP) kwa masabata a 3 (masiku 5 / sabata).Kukula kwa chotupacho kunayesedwa pa nkhwangwa ziwiri mothandizidwa ndi vernier caliper, ndipo kuchuluka kwa chotupa (mm3) kunawerengedwa.

Zolozera:
[1].Chen Y, ndi al.Pachymic acid imalepheretsa tumorigenesis m'maselo a gallbladder carcinoma.Int J Clin Exp Med.2015 Oct 15;8(10):17781-8.
[2].Ma J, ndi al.Pachymic acid imapangitsa apoptosis poyambitsa njira za ROS-zodalira JNK ndi ER m'maselo a khansa ya m'mapapo.Cancer Cell Int.2015 Aug 5; 15:78.