Zogulitsa

Kufotokozera:
prim-o-glucosylcimifugin ali ndi mphamvu zoletsa kutupa.Mafotokozedwe a iNOS ndi COX-2 akhoza kuletsedwa mwa kuwongolera JAK2 / STAT3 sign transd

Magawo oyenera:
Njira yowonetsera>> chitetezo ndi kutupa>> nitric oxide synthase
Kafukufuku>> kutupa / chitetezo chokwanira
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Zolinga:iNOS
COX-2

Phunziro la In Vitro:
prim-o-glucosylglycine (POG) ndizomwe zili pamwamba kwambiri mu chromone komanso chimodzi mwazinthu zomwe zimagwira ntchito mu Fangfeng (RS).Prim-o-glucosylglycine imagwira ntchito yolimbana ndi kutupa mu macrophages yaiwisi ya 264.7 poletsa kutulutsa kwa JAK2 / STAT3 ndikuletsa kufotokoza kwa iNOS ndi COX-2.The cytotoxicity ya prim-o-glucoside pa LPS activated yaiwisi 264.7 macrophages anayesedwa.Gwiritsani ntchito LPS (1 μ G / ml) ndikuchulukirachulukira kwa prim-o-glucosylglycine (15, 50 ndi 100 μ G / ml) kwa maola 24, ndipo mphamvu ya cell idawunikidwa ndi kuyesa kwa CCK-8.Poyerekeza ndi ma cell omwe amathandizidwa ndi DMSO (kuwongolera), maola 24 ndikuwonekera kwa 15-100 μ Pambuyo pa g / ml prim-o-glucoside, kuthekera kwa cell sikunakhudzidwe kwambiri.Kuti muphunzire za anti-inflammatory effect ya prim-o-glucosylglycine, kaya prim-o-glucosylglycine ingakhudze NO synthesis idawunikidwa mu LPS yotsegulidwa yaiwisi 264.7 maselo.Gwiritsani ntchito LPS (1 μ G / ml) ndi mitundu yosiyanasiyana ya prim-o-glucosylglycine (15, 50 ndi 100 μ G / ml) kwa maola 24.Kukhazikikako sikunayesedwe mu chikhalidwe chapamwamba kwambiri ndi machitidwe a Griess.Kuchuluka kwa ayi mu chikhalidwe chapamwamba kunakula kwambiri ndi kuwonekera kwa LPS, ndipo prim-o-glucoside yoletsa kwambiri LPS sinapangitse kuti pasakhale kupanga modalira ndende [1].
Mu Phunziro la Vivo
bronchoalveolar lavage fluid (BALF) inasonkhanitsidwa patatha maola 7 kuchokera pamene lipopolysaccharide (LPS) imayendetsedwa, ndipo mlingo wa cytokine mu BALF unayesedwa ndi ELISA.Poyerekeza ndi gulu lolamulira, TNF mu BALF- α, IL-1 β Ndi IL-6 milingo inakula kwambiri.Komabe, pretreatment ndi prime-o-glucosylglycine (2.5, 5 kapena 10 mg / kg) kwambiri pansi yolamulidwa TNF- α, IL-1 β Ndi IL-6 m'njira yodalira mlingo (P <0.05, P <0.05, 0.01) [1].

Kuyesera kwa Ma cell
Ma cell counting Kit (CCK-8) idagwiritsidwa ntchito kudziwa kuchuluka kwa cytotoxic ya prim-o-glucosylglycine.Mwachidule, maselo aiwisi a 264.7 amathandizidwa ndi 1 pachitsime × Kuchulukana kwa maselo a 104 kunalowetsedwa m'zitsime za 96 ndikuyikidwa usiku wonse.Kenako gwiritsani ntchito 1 μ Maselo adalimbikitsidwa ndi g / ml LPS ndikuthandizidwa ndi mitundu yosiyanasiyana ya prim-o-glucosylglycine (15, 50 ndi 100 μ g/mL; Medchem Express, Princeton, NJ, USA) kapena chithandizo cha DMSO.Pambuyo pa makulitsidwe pa 37 ℃ kwa maola 24, yankho la CCK-8 linawonjezedwa pa chitsime chilichonse ndikuumirira kwa ola lina.Absorbance anayesedwa pa 450 nm pogwiritsa ntchito microplate reader [1].

Kuyesera kwa Zinyama
mbewa [1] BALB / C mbewa zamphongo, masabata 8, zolemera pafupifupi 18 mpaka 20g.Mbewa zinagawidwa mwachisawawa m'magulu a 5: gulu lolamulira;gulu la LPS;LPS + prime-o-glucosylglycine (2.5, 5 kapena 10mg / kg kulemera kwa thupi).Prime-o-glucosylglycine amaperekedwa intraperitoneally.Pambuyo pa ola la 1, mbewa mu gulu la LPS ndi LPS + prime-o-glucosylglycine gulu anapatsidwa 50 mg / L intranasal (mu) (200 mg / L) μ LLPs kuchititsa kuvulala pachimake m'mapapo.Makoswe owongolera anapatsidwa 50% intranasal (mu) popanda LPS μ LPBS
[1]

Zolozera:
[1].Zhou J, ndi al.Prim-O-glucosylcimifugin Attenuates LipopolysaccharideInduced Inflammatory Response mu RAW 264.7 Macrophages.Pharmacogn Mag.2017 Jul-Sep; 13 (51): 378-384.
[2].Chen N, ndi al.Prime-O-glucosylcimifugin amalepheretsa kuvulala koopsa kwa mapapo a lipopolysaccharide mu mbewa.Mankhwala a Immunopharmacol.2013 Jun; 16 (2): 139-47.