Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
Zambiri Zofunikira
Salvianolic acid B ndi condensation wa mamolekyu atatu a Danshensu ndi molekyu imodzi ya caffeic acid.Ndi imodzi mwazophunzira kwambiri za salvianolic acid.Lili ndi zotsatira za pharmacological pamtima, ubongo, chiwindi, impso ndi ziwalo zina.Chogulitsachi chimakhala ndi zotsatira zolimbikitsa kufalikira kwa magazi ndikuchotsa ma stasis amagazi, dredging meridians ndikuyambitsa zomangira.Amagwiritsidwa ntchito kwambiri pochiza sitiroko ya ischemic yomwe imayamba chifukwa cha kutsekeka kwa magazi komwe kumatsekereza meridians, monga dzanzi la theka la thupi ndi miyendo, kufooka, kupweteka kwamgwirizano, kulephera kwa magalimoto, kupotoza pakamwa ndi maso, ndi zina zambiri.
Dzinali:salvianolic acid B, salvianolic acid B, salvianolic acid B
Dzina la Chingerezi:salvianolic acid B
Molecular formula:c36h30o16
Kulemera kwa mamolekyu:718.62
Nambala ya CAS:115939-25-8
Njira yodziwira:HPLC ≥ 98%
Zofotokozera:10mg, 20mg, 100mg, 500mg, 1g (akhoza mmatumba malinga ndi zofunika kasitomala)
Ntchito ndi kugwiritsa ntchito:Izi zimagwiritsidwa ntchito pozindikira zomwe zili.
Thupi ndi mankhwala katundu
Katundu:mankhwala ndi quasi woyera ufa.
Kukoma kwake kumakhala kowawa pang'ono komanso kowawa, komwe kumapangitsa chinyezi.Kusungunuka m'madzi, ethanol ndi methanol.
Salvianolic acid B amapangidwa ndi condensation wa 3 mamolekyu a salvianolic acid ndi 1 molekyu ya caffeic acid.Ili ndi magulu awiri a carboxyl ndipo ilipo mu mawonekedwe a mchere wosiyanasiyana (K +, Ca2 +, Na +, NH4 +, etc.).Mu ndondomeko Decoction ndi ndende, gawo laling'ono la salvianolic asidi B ndi hydrolyzed kuti chibakuwa oxalic asidi ndi salvianolic asidi, ndi gawo la salvianolic asidi B amakhala rosmarinic asidi pansi acidic zinthu;Salvianolic acid A ndi C akhoza kukhala tautomeric mu yankho.
Zofotokozera
＞5%,＞10%,＞50%,＞70%,＞90%,98%
M'zigawo Njira
Radix Salviae Miltiorrhizae inaphwanyidwa, kuikidwa mu thanki yochotsamo, yonyowetsedwa ndi 8 kuwirikiza kuchuluka kwa 0.01mol/l hydrochloric acid usiku wonse, ndiyeno kuphwanyidwa ndi 14 kuchuluka kwa madzi.Yankho lopangidwa ndi percolated limatsukidwa ndi AB-8 macroporous resin column.Choyamba, elute ndi 0.01mol/l hydrochloric acid kuchotsa zonyansa sanali adsorbed, ndiyeno elute ndi 25% Mowa kuchotsa kwambiri polar zosafunika.Pomaliza, kuyang'ana 40% Mowa eluent pansi pa kupanikizika kuchepetsa kuti achire Mowa ndi amaundana-zouma kupeza okwana Salvia miltiorrhiza phenolic asidi ndi chiyero cha oposa 80%.
Dziwani
Tengani 1g ya mankhwala, pogaya, onjezani 5ml wa Mowa, kusonkhezera kwathunthu, fyuluta, kutenga madontho ochepa a filtrate, dontho pa pepala fyuluta mzere, ziwunikeni, kuona pansi pa nyali ultraviolet (365nm), onetsani buluu- imvi fluorescence, ponyani pepala fyuluta mu anaikira ammonia yankho botolo (osakhudza madzi pamwamba), itulutseni pambuyo mphindi 20, onani pansi pa nyali ultraviolet (365nm), kusonyeza blue-wobiriwira fulorosenti.
Acidity:tengani yankho lamadzi pansi pa chinthu chomveka bwino, ndipo pH mtengo udzakhala 2.0 ~ 4.0 (zowonjezera za Chinese Pharmacopoeia Edition 1977).
Kutsimikiza Kwazinthu
Zinatsimikiziridwa ndi chromatography yamadzimadzi (Zowonjezera VI D, Volume I, Chinese Pharmacopoeia, 2000 EDITION).
Octadecyl silane womangidwa silika gel osakaniza ankagwiritsidwa ntchito monga filler mu chromatographic mikhalidwe ndi dongosolo applicability mayeso;Methanol acetonitrile formic acid madzi (30:10:1:59) anali gawo loyenda;Kutalika kwa mawonekedwe kunali 286 nm.Chiwerengero cha mbale zongoyerekeza zowerengedwa molingana ndi salvianolic acid B pachimake sichiyenera kuchepera 2000.
Kukonzekera yankho lolozera molondola kuyeza kuchuluka koyenera kwa salvianolic acid B yankho lofotokozera ndikuwonjezera madzi kuti likhale ndi 10% pa 1ml μ G yankho.
Kukonzekera yankho mayeso kutenga za 0.2g wa mankhwala, molondola kulemera, anaika mu 50ml kuyeza botolo, kuwonjezera mlingo woyenera wa methanol, sonicate kwa mphindi 20, kuziziritsa izo, kuwonjezera madzi sikelo, kugwedeza bwino, fyuluta. Ilo, yezani molondola 1ml ya kusefa kosalekeza, ikani mu botolo loyezera 25ml, onjezerani madzi pamlingo, gwedezani bwino.
Njira yotsimikizika imatenga 20% ya njira yowongolera ndi 20% ya mayeso μ l.Ibayeni mu chromatograph yamadzi kuti mudziwe.
Pharmacological Efficacy
Salvianolic acid B ndi condensation wa mamolekyu atatu a Danshensu ndi molekyu imodzi ya caffeic acid.Ndi imodzi mwazophunzira kwambiri za salvianolic acid.Lili ndi zotsatira za pharmacological pamtima, ubongo, chiwindi, impso ndi ziwalo zina.
Antioxidant
Salvianolic acid B ali ndi mphamvu ya antioxidant.Zoyeserera mu vivo ndi mu vitro zikuwonetsa kuti salvianolic acid B imatha kuwononga ma radicals opanda okosijeni ndikuletsa lipid peroxidation.Kuchita kwake kwakukulu ndikwapamwamba kuposa vitamini C, vitamini E ndi mannitol.Ndi imodzi mwazinthu zodziwika bwino zachilengedwe zomwe zimakhala ndi antioxidant wamphamvu kwambiri Maphunziro a Pharmacological akuwonetsa kuti salvianolic acid ya jekeseni imakhala ndi antioxidant kwenikweni, imalepheretsa kuphatikizika kwa mapulateleti ndi thrombosis, ndipo imatha kutalikitsa nthawi yamoyo ya nyama zomwe zili pansi pa hypoxia.Zotsatira zake zidawonetsa kuti salvianolic acid ya jakisoni (60 ~ 15mg / kg) imatha kusintha kwambiri kuperewera kwaubongo mu makoswe omwe ali ndi vuto la cerebral ischemia-reperfusion, kuwongolera kusokonezeka kwamakhalidwe komanso kuchepetsa kwambiri gawo la ubongo wa infarction.Panali kusiyana kwakukulu pakati pa mlingo waukulu ndi wapakati (60 ndi 30mg / kg);Salvianolic acid ya jekeseni imatha kusintha kwambiri kuwonongeka kwa mitsempha chifukwa cha FeCl3 imayambitsa ubongo wa ischemia mu makoswe pa 1, 2 ndi maola a 24 pambuyo pa utsogoleri, zomwe zimawonetseredwa pakusintha kwa khalidwe lachidziwitso ndi kuchepetsa dera la cerebral infarction;Salvianolic acid 40 mg/kg kwa jakisoni kwambiri inhibited kuphatikizika kwa kalulu kupatsidwa zinthu za m`mwazi anachititsa ndi ADP, asidi arachidonic ndi kolajeni, ndi chopinga mitengo anali 81.5%, 76,7% ndi 68.9% motero.Salvianolic acid 60 ndi 30mg/kg kwa jekeseni kwambiri linaletsa thrombosis mu makoswe;Salvianolic acid 60 ndi 30mg / kg ya jakisoni imatalikitsa nthawi yopulumuka ya mbewa pansi pa hypoxia.
Ntchito Yachipatala
Chogulitsachi chimakhala ndi zotsatira zolimbikitsa kufalikira kwa magazi ndikuchotsa ma stasis amagazi, dredging meridians ndikuyambitsa zomangira.Amagwiritsidwa ntchito kwambiri pochiza sitiroko ya ischemic yomwe imayamba chifukwa cha kutsekeka kwa magazi komwe kumatsekereza meridians, monga dzanzi la theka la thupi ndi miyendo, kufooka, kupweteka kwamgwirizano, kulephera kwa magalimoto, kupotoza pakamwa ndi maso, ndi zina zambiri.
Sitolo
Pamalo ozizira ndi owuma.
Nthawi Yovomerezeka
Zaka ziwiri.
Njira Yosungira
2-8 ° C, yosungidwa m'malo ozizira komanso owuma komanso kutali ndi kuwala.
Nkhani Zofunika Kusamala
Mankhwalawa ayenera kusungidwa kutentha kochepa.Ngati ziwululidwa ndi mpweya kwa nthawi yayitali, zomwe zilimo zimachepetsedwa.
