Products

Description:
prim-o-glucosylcimifugin has potent anti-inflammatory effects. The expression of iNOS and COX-2 can be inhibited by regulating JAK2 / STAT3 signal transd

Relevant categories:
Signaling pathway > > immunity and inflammation > > nitric oxide synthase
Research field > > inflammation / immunity
Natural Products > > others

Target: iNOS
COX-2

In Vitro Study:
prim-o-glucosylglycine (POG) is the highest content of chromone and one of the main active components in Fangfeng (RS). Prim-o-glucosylglycine plays an anti-inflammatory role in raw 264.7 macrophages by inhibiting JAK2 / STAT3 signal transduction and inhibiting the expression of iNOS and COX-2. The cytotoxicity of prim-o-glucoside on LPS activated raw 264.7 macrophages was measured. Use LPS (1 μ G / ml) and increasing concentrations of prim-o-glucosylglycine (15, 50 and 100 μ G / ml) for 24 hours, and cell viability was evaluated by CCK-8 assay. Compared with DMSO treated cells (control), 24 hours and exposure to 15-100 μ After g / ml prim-o-glucoside, cell viability was not significantly affected. In order to study the anti-inflammatory effect of prim-o-glucosylglycine, whether prim-o-glucosylglycine can affect NO synthesis was examined in LPS activated raw 264.7 cells. Use LPS (1 μ G / ml) and various concentrations of prim-o-glucosylglycine (15, 50 and 100 μ G / ml) for 24 hours. The concentration was not measured in the culture supernatant by Griess reaction. The concentration of no in the culture supernatant increased significantly with LPS exposure, and prim-o-glucoside significantly inhibited LPS induced no production in a concentration dependent manner [1].
In Vivo Study
bronchoalveolar lavage fluid (BALF) was collected 7 hours after lipopolysaccharide (LPS) administration, and the cytokine level in BALF was measured by ELISA. Compared with the control group, TNF in BALF- α， IL-1 β And IL-6 levels increased significantly. However, pretreatment with prime-o-glucosylglycine (2.5, 5 or 10 mg / kg) significantly down regulated TNF- α， IL-1 β And IL-6 in a dose-dependent manner (P < 0.05, P < 0.05, 0.01) [1].

Cell Experiment
cell counting Kit (CCK-8) was used to determine the cytotoxic concentration of prim-o-glucosylglycine. In short, raw 264.7 cells were treated with 1 per well × The density of 104 cells was inoculated in 96 wells and incubated overnight. Then use 1 μ Cells were stimulated with g / ml LPS and treated with various concentrations of prim-o-glucosylglycine (15, 50 and 100 μ g/ mL; Medchem express, Princeton, NJ, USA) or DMSO treatment. After incubation at 37 ℃ for 24 hours, CCK-8 solution was added to each well and incubated for another hour. Absorbance was measured at 450 nm using a microplate reader [1].

Animal Experiment
mice [1] BALB / C male mice, 8 weeks old, weighing about 18 to 20g. Mice were randomly divided into 5 groups: control group; LPS group; LPS + prime-o-glucosylglycine (2.5, 5 or 10mg / kg body weight). Prime-o-glucosylglycine was administered intraperitoneally. After 1 hour, mice in LPS group and LPS + prime-o-glucosylglycine group were given 50 mg / L intranasal (in) (200 mg / L) μ LLPs to induce acute lung injury. The control mice were given 50% intranasal (in) without LPS μ LPBS
[1]

Reference:
[1]. Zhou J, et al. Prim-O-glucosylcimifugin Attenuates Lipopolysaccharideinduced Inflammatory Response in RAW 264.7 Macrophages. Pharmacogn Mag. 2017 Jul-Sep;13(51):378-384.
[2]. Chen N, et al. Prime-O-glucosylcimifugin attenuates lipopolysaccharide-induced acute lung injury in mice. Int Immunopharmacol. 2013 Jun;16(2):139-47.