Glycyrrhizin, Liquiritin;Liquiritoside;Likviritin;Liquiritoside Cas No.551-15-5
Intangiriro
Glycyrrhizin, izwi kandi nka Liquiritin.Licorice ni igihingwa cya Glycyrrhiza muri Leguminosae.Imizi n'ibiti byayo ni ibimera bisanzwe byabashinwa.
Ubuvuzi bukwirakwizwa cyane mu majyaruguru y'uburasirazuba bw'Ubushinwa, Sinayi, Yunnan, Mongoliya y'imbere, Anhui n'ahandi.Shennong materia medica classic yashyize ku rutonde nkurwego rwo hejuru, agira ati "iki cyatsi ni umwami wimiti yose, kandi ni bake batagikoresha".Uruhushya rufite ibice bigoye, cyane cyane birimo triterpenoide, flavonoide na coumarine.Flavonoide ni ubwoko bwa bioactive ibice byakuwe mubikomoka ku bishishwa.Ibigize imiti bivura cyane cyane harimo glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, nibindi. Mu myaka yashize, haravuzwe amakuru avuga ku buntu bwangiza, antioxydants, anti-kanseri ndetse n’ingaruka zo kurwanya mutagenic flavonoide mu gihugu ndetse no mu mahanga.
ImitiName:4H-1-Benzopyran-4-imwe, 2- [4- (β-D-glucopyranosyloxy) fenyl] -2, 3-dihydro-7-hydroxy-, (S)
PhysicalPubugeni:Monohydrate (dilute etanol cyangwa amazi), aho gushonga: 212 ~ 213 ° ℃.
Igikorwa cya farumasi
Uburozi: Nta
Ingaruka mbi: itazwi
Inkomoko yibikoresho: ibinyamisogwe Glycyrrhiza glabra L. umuzi, Glycyrrhiza uralensis Fisch Imizi.
Gukuramo Glycyrrhizin
Gutegura Ibikoresho bya Licorice
Ibigize imiti yibikoresho byibanze biragoye cyane.Kugirango ubone ingaruka nziza zo gutandukana, gabanya umwanda wanduye kumurongo wateguwe wa chromatografiya, no kunoza ibirimo glycyrrhizin mubikoresho byatewe inshinge, uburyo bwo kuvoma bwakoreshejwe mukubeshya ibikoresho bibisi.Gupima ibyatsi bya 4G hamwe nuburinganire bwa elegitoronike hanyuma ubishyire muri beaker.Gupima neza amazi 100ml yatoboye hamwe na silinderi yo gupima hanyuma uyisuke muri beaker kugirango ushonga.Ultrasonic muminota igera kuri 15, kandi uhore ukangisha inkoni yikirahure kugirango wihute.Noneho shyira inzoga mu bwogero bwa 90 ℃ thermostatike hanyuma ubishyuhe amasaha 2, hanyuma ubishyuhe kugirango ubyungurure.Nyuma yo kongeramo akayunguruzo mumashanyarazi ya n-butanol no guhagarara muminota mike, glycoside nyinshi zishonga mumashanyarazi ya n-butanol, hanyuma zigakuramo kabiri, zikuramo glycoside nkeya zisigaye mumazi, hanyuma ugahuza hanyuma ugahuriza hamwe n- igisubizo cya butanol cyabonetse mugukuramo kabiri kuri chromatografiya no kwezwa。
Isuku rya Glycyrrhizin Na Chromatography
Fata ml 10 yibicuruzwa byakuwe hejuru nkibikoresho fatizo byabigenewe, tangira pompe, shyira umuvuduko wa 25 ml / min, hanyuma uzane ibikoresho bibisi muri mm 500 ukoresheje mobile mobile (methanol: amazi = 1: 4) × Muri a Inkingi ya mm 40 yo gutegura, kusanya igice cyibicuruzwa bya glucoside ukurikije ibihe byimpera: igice cya 1 h cyambere cyegeranijwe hamwe nkigice cyambere cyanduye, hanyuma uhindure imigezi.Kurugero, oza inkingi hamwe nuruvange rwa 50% methanol namazi, uhuze ibicuruzwa buri min 20, hanyuma ushire hamwe icupa ryibicuruzwa hamwe no guhumeka, hanyuma ufate 20 µ L kugirango isesengura rya chromatografi ya HPLC, Kugeza nta ntego igaragara.Imiterere ya HPLC yari iteye itya: icyiciro kigendanwa: methanol: amazi = 3.5: 6.5;Icyiciro gihagaze: silika gel karubone 18;Inkingi ya Chromatografiya: 450 mm × 4,6 mm rate Igipimo cyo gutemba: 1 ml / min;Uburebure bwumurongo: 254nm.Ibiri muri glycyrrhizin mu icupa rya kabiri nibyo byinshi mubicuruzwa byakiriwe buri min 20
Isuku rya Glycyrrhizin Na Rechromatography
Kubera ko ibikubiye muri glycyrrhizin nyuma yo kweza kwa chromatografiya itari hejuru, uburyo bumwe bwatoranijwe.Fata ml 10 yibicuruzwa byavuzwe haruguru bisukuwe nkibikoresho fatizo bihagaze neza, umuvuduko wa 25 ml / min, hanyuma uzane icupa rya kabiri ryibicuruzwa muri mm 500 ukoresheje icyiciro cya mobile (methanol: amazi = 2: 5) × Muri mm 20 column inkingi ya chromatografi, kusanya distillate yibicuruzwa byatsi glycoside ukurikije uko ibintu bimeze: guhuza ibicuruzwa buri min 4, hanyuma ushire hamwe icupa ryibicuruzwa hamwe no guhumeka, hanyuma ukoreshe umurongo umwe wo gutahura hejuru kugirango HPLC isesengure kugeza igihe nta ntego ihari. .Nyuma yisesengura, byagaragaye ko ibikubiye muri glycyrrhizin mu icupa rya gatandatu aribyo byari hejuru cyane mubicuruzwa byakiriwe buri min 4, aho igihe cyo kugumana cyari min 5.898 min nkimpanvu yagenewe, naho ibirimo bigera kuri 40% muburyo bwo guhuza ubusanzwe .
Kohereza Ibicuruzwa
Ibicuruzwa byakusanyirijwe hamwe bitandukanijwe nigitutu cyagabanutse kuri moteri izenguruka kuri 70 ℃.Umuti umaze guhumeka cyane, shonga ibicuruzwa bikomeye kumurambararo wuruziga hamwe na methanol nkeya, hanyuma uhindurwe mumiyoboro yipimisha mubushyuhe bwicyumba kugeza kristu yera yera igaragara [2].
