Ibicuruzwa

Intego:JNK1 jnk2 NF- κ B.

Mu bushakashatsi bwa vitro:isovitexin irinda LPS iterwa na okiside yangiza muguhagarika umusaruro wa ROS idasanzwe, kandi ikanagabanya ingaruka za H2O2 kumikorere ya selile.Harimo LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) nta cytotoxicitike yagiraga selile 264.7, ariko 200 μ G / ml isovitexin yerekanaga cytotoxicity ikomeye.Isovitexin (25,50) μ G / ml) yabujije LPS itera TNF- α ， Kongera urwego rwa IL-6, iNOS na COX-2.Isovitexin (25,50) μ G / ml) nayo yabujije I mu ngirabuzimafatizo mbisi 264.7 κ B α Fosifora no gutesha agaciro, ibyo bikaba bihuye n'ingaruka za JNK1 / 2 inhibitor [1].

Mu bushakashatsi bwa vivo:isovitexin (50 na 100 mg / kg, IP) yateje impinduka nke za histopathologique mu bice by'ibihaha kandi igabanya umubare w'uturemangingo twangiza muri LPS yatewe n'imbeba.Heteroeosinophile (50 na 100 mg / kg, IP) yagabanije TNF by- α Kandi umusaruro wa IL-6, umusaruro wa ROS, MPO na MDA, wongera SOD na GSH, kandi wirinda LPS itera umuriro hamwe na stress ya okiside muri LPS itera imbeba za Ali.Kandi uhagarike neza imvugo ya protein ya iNOS na COX-2 [1].Isovitexin (25,50, 100 mg / kg) yagabanije ubuzima bwo kubaho kwa LPS / D-gal yatewe n'umwijima ku mbeba mu buryo butandukanye.Isovitexin kandi yabujije NF- κ B no hejuru igenga LPS / D-gal itera Nrf2 na HO-1 mu mbeba [2].

Ubushakashatsi bwakagari:selile viability yagenwe na MTT assay.Ingirabuzimafatizo 264.7 zatewe ku isahani 96 y’iriba (1) × 104 selile / iriba) hamwe n’ubushakashatsi butandukanye bwa isovitexine (kwibanda kwa nyuma: 0-200) μ G / ml) na LPS (2 μ G / ml) mu masaha 24.Wongeyeho, koresha IV (25 cyangwa 50 μ G / ml) wateguye selile kumasaha 1, hanyuma H 2O 2 (300) hiyongeraho μ M）。 Nyuma yamasaha 24, MTT (5 mg / ml) yongewe kuri selile hanyuma ikabikwa amasaha 4 [1].

Ubushakashatsi ku nyamaswa:imbeba. ), LPS (0.5 mg / kg) + isovitexin (50 cyangwa 100 mg / kg) na LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, yashonga muri saline).Isovitexin cyangwa DEX (5 mg / kg) isovitexin yatanzwe.Nyuma yo guhura na isovitexin cyangwa DEX kumasaha 1, imbeba zatewe aneste hamwe na ether hanyuma LPS itangwa imbere (muri) kugirango ikomeretsa ibihaha.Amatungo yatanzwe nyuma yamasaha 12 ubuyobozi bwa LPS.Kubwibyo, bronchoalveolar lavage fluid (BALF) hamwe nuduce twibihaha byasaruwe kugirango bapime urugero rwa cytokine;Igisekuru cya ROS;Ibikorwa bya SOD, GSH, MDA na MPO;Kandi imvugo ya poroteyine ya COX-2, iNOS, HO-1 na Nrf2 [1].