Ibicuruzwa

Intego:GABA yakira, mTOR, PI3K, Akt [1] [2]

Mu bushakashatsi bwa vitro:ikigereranyo gito 41 μ Jujuboside A ya M (hafi 0,05 g / L) yateye reseptor ya GABA (a) mugihe cyo kuvura 24 na 72 h α 1 ， α 5 ， β 2 subunit mRNA yiyongereye cyane.Igipimo kinini 82 μ M (hafi 0.1 g / L) Jujuboside A yiyongereye cyane yakira GABA (a) yakira 24 h α 1 ， α 5 subunit ya mRNA kandi igabanuka β 2 subunit mRNA kandi igabanya subunit ya GABA (a) reseptor subunit α 1 ， expression imvugo ya mRNA yavuwe kuri 72 h [1].Jujuboside Kwiyitirira birashobora guhindura igabanuka ryimikorere ya selile kandi bigatera kurushaho ISO gukomeretsa H9c2.Jujuboside A irashobora kwihutisha fosifora ya PI3K, Akt na mTOR.Jujuboside A irashobora kugabanya cyane igipimo cya microtubule ifitanye isano na poroteyine LC3-II / I muri selile H9c2 [2]

Mu bushakashatsi bwa vivo:ku manywa (9: 00-15: 00), jujuboside yongereye cyane ibitotsi byose hamwe no gusinzira byihuse (REM), ariko ntibyagize ingaruka zikomeye kubitotsi bitari REM (NREM).Mwijoro (21: 00-3: 00), jujuboside yongereye cyane ibitotsi byose hamwe no gusinzira kwa NREM, cyane cyane ibitotsi byoroheje, ariko ntibyagize ingaruka zikomeye kubitotsi bya REM no gusinzira buhoro (SWS) [3].Kuvura imitsi hamwe na jjuboside a byagabanutse cyane binyuze muri Y-maze, kwirinda cyane hamwe no gupima amazi ya Morris β 1-42 byatewe no kwiga no kutagira ubwenge mu mbeba.Gutera inshinge za Jujuboside A kugabanuka kwa hippocampal urwego β 1-42 byabujije cyane ibikorwa bya acetylcholinesterase (AChE) na oya, kandi byagabanije kwiyongera kwa malondialdehyde (MDA) muri hippocampus na cortex yubwonko bwimbeba zatewe mumashanyarazi β 1-42 [4]。

Ubushakashatsi bwakagari:nyuma yiminsi 7 yumuco muri vitro, selile zahoraga zigaragara kumasaha 24 cyangwa amasaha 72, kandi ntizagaragaye muburyo burimo Jujuboside A cyangwa diazepam cyangwa byombi.Vector yongerewe mumatsinda yo kugenzura;Itsinda rya Diazepam ryongeyeho 10 μ M diazepam;Jujuboside A82 μ M (hafi 0.1g / L) na 41 μ M (hafi 0,05 g / L) yongewe kuri jua-h (ikinini kinini Jujuboside A) na jua-l (dose ya Jujuboside A).RNA yose yatandukanijwe na selile kugirango isesengurwe [1].

Ubushakashatsi ku nyamaswa:imbeba: a yatewe muri ICV β 1-42 itera ubumuga bwo kutamenya mu mbeba.Hanyuma, imbeba zatewe na Jujuboside A (0.02 na 0.2 mg / kg) imitsi (ICV) muminsi 5.Y maze, kwirinda cyane hamwe na Morris water maze ibizamini byakorewe ku mbeba [1].