Ibicuruzwa

Ibisobanuro:
acide pachymic ni triterpenoid kuva P. cocos.Acide Pachymic ibuza Akt na ERK inzira yerekana inzira.

Ibyiciro bifitanye isano:
urwego rw'ubushakashatsi >> kanseri
Ibicuruzwa bisanzwe >> terpenoide na glycoside

Intego:
Akt
ERK

Mu Kwiga Vitro:
acide pachymic (PA) irashobora guhagarika tumorigenez ya kanseri yo mu mara irimo Akt na ERK byerekana inzira.Ubuvuzi bwa pachymic (PA) bwabujije cyane inzira ya Rho a, Akt na ERK mu ngirangingo za kanseri ya gallbladder.Pachymic acide (PA) irashobora kugabanya PCNA, ICAM-1, RhoA, p-Akt na perk muburyo buterwa na dose.Nyuma yamasaha 12 nyuma yo kuvurwa, 10 μ G / ml Acide Poria (PA) yabujije imikurire ya selile yibice 30 μ G / ml bikomeza kugabanya imikurire ya selile.Gukura kwakagari kwabujijwe mugihe - kandi muburyo butandukanye.Nyuma yamasaha 48 yo kuvura, intumbero yari 10 μ g / mL ， 20 μ G / ml na 30 μ acide Pachymic (PA) kuri g / ml yabujije imikurire ya selile hafi 25%, 40% na 70%.Acide Pachymic (PA) nayo ibuza imikurire ya kanseri ya gallbladder kanseri iterwa nigihe kandi iterwa na dose [1].

Mu Kwiga Vivo:
hagamijwe gusuzuma ibikorwa birwanya aside pachymic aside (PA) muri vivo, hakoreshejwe kanseri yibihaha yumuntu nci-h23 ikibyimba xenograft.Acide Pachymic (PA) yabujije cyane gukura kw'ibibyimba ku kigero cya 30 na 60 mg / kg mu minsi 21 ugereranije n'itsinda rishinzwe kugenzura [2].

Ubushakashatsi bwakagari:
kubara selile-8 (CCK-8) yakoreshejwe mugusuzuma ingaruka zo gukwirakwiza aside pachymic (PA) kuri selile GBC-SD.Muri make, nyuma yubuvuzi bwagenwe, ongeramo 10 kuri buri mwobo μ Lcck-8 igisubizo, hanyuma nyuma yubushakashatsi bumwe, iyinjizwa ryapimwe kuri 450 nm ukoresheje umusomyi wa microplate [1].

Ubushakashatsi ku nyamaswa:
imbeba [2] yakoresheje imbeba zambaye ubusa zumugore zifite ibyumweru 4-5.Byakuze cyane nci-h23 selile (kuri 100 μ 5 muri LPBs × 106) byatewe muburyo bwihuse muburyo bwiburyo bwa buri mbeba.Tumor xenografts yakuze kugeza ku kigereranyo cya 100-200mm 3 hanyuma igahita ihabwa amatsinda ane yo kuvura (imbeba 6 muri buri tsinda): (a) kugenzura ibinyabiziga (0.1% DMSO muri saline isanzwe);（B acid Acide Pachymic （PA） 10 mg / kg;C） PA 30 mg / kg;Dd） PA 60mg / kg ice Imbeba zahawe pa ninshinge ya intraperitoneal (IP) ibyumweru 3 (iminsi 5 / icyumweru).Ingano yikibyimba yapimwe kumashoka abiri hifashishijwe vernier caliper, hanyuma ibibyimba (mm3) birabaze.

Reba:
[1].Chen Y, n'abandi.Acide Pachymic ibuza tumorigenez mu ngirabuzimafatizo ya kanseri.Int J Clin Yagutse Med.2015 Ukwakira 15; 8 (10): 17781-8.
[2].Ma J, n'abandi.Acide Pachymic itera apoptose binyuze mu gukora inzira ya ROS iterwa na JNK na ER inzira ya kanseri yibihaha.Kanseri Int.2015 Kanama 5; 15: 78.