Ibicuruzwa

Gutegura igisubizo cyifashishwa:fata urugero rukwiye rwibisubizo bya Ruscogenin, ubipime neza, hanyuma ushyiremo methanol kugirango bikore birimo 50% kumuti wa 1ml μ G. ml yumuti wibisubizo, ubishyire mumashanyarazi hamwe na stopper, hanyuma uhumure umusemburo mubwogero bwamazi.Ongeraho neza acide 10ml perchloric, uyinyeganyeze neza, uyibike mumazi ashyushye muminota 15, uyikuremo, uyikonje n'amazi ya barafu, fata reagent ijyanye nubusa, upime iyinjizwa ryumurambararo wa 397nm ukurikije ultraviolet igaragara ya spekitifotometometrie ( Umugereka VA), fata ibyinjira nkibisanzwe hamwe nibitekerezo nka abscissa, hanyuma ushushanye umurongo usanzwe.

Gutegura igisubizo cyibizamini:fata ifu nziza ya 3G yibicuruzwa, ubipime neza, ubishyire mumashanyarazi ya conic hamwe na stoper, ongeramo neza 50ml ya methanol, ubipime, ubushyuhe nibisubiramo mumasaha 2, ubikonje, ubipime, ugire ibiro byatakaye hamwe na methanol, kunyeganyeza neza no kuyungurura.Gupima neza 25ml ya filtrate ikomeza, uyishyire muri flask, usubize umusemburo wumye, ongeramo 10ml y'amazi kugirango ushongeshe ibisigazwa, uyuzuze amazi, uyinyeganyeze na n-butanol inshuro 5, 10ml buri mwanya, uhuze n -umuti wa butanol, kwoza kabiri hamwe nigisubizo cya ammonia, 5ml buri gihe, ujugunye igisubizo cya ammonia, hanyuma uhumure n-butanol yumuti.Kuramo ibisigara hamwe na methanol 80% hanyuma ubyohereze kuri flask ya 50ml ya volumetric, ongeramo methanol 80% mubipimo hanyuma uzunguze neza.

Uburyo bwo kumenya neza gupima neza 2 ~ 5ml yumuti wikizamini, ukabishyira muri 10ml wacometse mu cyuma cyumye, ukurikije uburyo buteganijwe gutegurwa umurongo usanzwe, gupima iyinjizwa hakurikijwe amategeko kuva "guhindagura umusemburo mu bwogero bw’amazi", soma ingano ya ruscoegenin mugisubizo cyikizamini uhereye kumurongo usanzwe hanyuma ubare.

Saponine yose ya Ophiopogon japonicus ntishobora kuba munsi ya 0,12% ishingiye kuri Ruscogenin (C27H42O4).

Imiterere ya Chromatografiya: (kubisobanuro gusa)