Ibicuruzwa

Tifonine glycoside

Puhuang ni amabyi yumye ya Typha angustifolia L., Typha Orientalis Presl cyangwa ibimera bisa na Tifase.Ibiri muri isorhamnetin-3-o-neohesperidin muri Pharmacopoeia yo mu Bushinwa (2005 Edition) bigenwa na HPLC.Uburyo nyamukuru bwo kumenya isorhamnetin-3-o-neohesperidin muri Tifayike no kuyitegura ni

HPLC.Ukurikije ibikubiye muri Tifa muri Pharmacopoeia yo mu Bushinwa (Edition 2005):

Imiterere ya Chromatografiya hamwe na sisitemu ikoreshwa: octadecyl silane ihujwe na silika gel nkuwuzuza;Amazi ya Acetonitrile (15:85) yakoreshejwe nk'icyiciro kigendanwa;Uburebure bwumurambararo bwari 254 nm.Ubushyuhe bwinkingi 40 ℃.Umubare w'ibyapa byerekana ntushobora kuba munsi ya 1000 ukurikije impinga ya isorhamnetin-3-o-neohesperidin.

Gutegura igisubizo cyibizamini: fata hafi 0.5g yibi bicuruzwa, ubipime neza, ubishyire mu icupa ripima 50ml, ongeramo 45ml ya methanol, kuvura ultrasonic (power 250W, frequency 20KHz) muminota 30, bikonje, ongeramo methanol kuri igipimo, kunyeganyeza neza, kuyungurura, no gufata akayunguruzo gahoraho

Kumenya isorhamnetin-3-o-neohesperidin muri Puhuang nimyiteguro yayo na HPLC.Sisitemu rusange igendanwa ni amazi ya acetonitrile:

① Hp1050 ikora neza ya chromatografi;Inkingi ya Chromatografique C18 (5) μ m, 4,6mm × 250mm) Ishuri rya Dalian Institute of Chemical Physics, Academy of Science of China);Icyiciro kigendanwa: amazi ya acetonitrile (15:85);Uburebure bwumurongo: 254nm;Ubushyuhe bwa parike.

Chromatografi ikora cyane ni sisitemu ya hp1050 ya Hewlett Packard Company muri Amerika;Inkingi ya Chromatografique inkingi 10 C18 (3.9mm) × 300mm phase phase Icyiciro cya mobile: amazi acetonitrile (80:20);gutahura uburebure bwa 254 nm, umurongo wa 4 nm;uburebure bwa 550 nm, umurongo wa 100 nm;attenuation 8.

Fu Daxu n'abandi.Kumenya ibiri muri typhoniflorin na isorhamnetin-3-o-neohesperidin mumashanyarazi yose ya flavone ya Tifa na HPLC na 1100 chromatograf.Inkingi ya chromatografi ni ubwirakabiri XdB C18 (250mm × 4,6mm ， 5 μ m) phase Icyiciro cya mobile: acetonitrile-0.5% acide acetike methanol ikuraho (0min: 15: 78: 7; 15min: 23: 70: 7);Uburebure bwumurongo ni 248 nm.Gutegura ibimera byose bya flavonoide ya Tifa: 50g ya Tifa yakuwe hamwe na 60% Ethanol (500ml) × 2), 1H buri gihe, ibivamo byibanze kuri 40 ℃, ibiyikuramo byashongeshejwe namazi 600ml hanyuma bigashyirwa hagati.Ibisigara byashongeshejwe hamwe na 300ml y'amazi hanyuma bigashyirwa hamwe.Huza igisubizo cya centrifugal, shyira amajwi kuri 1000ml n'amazi, uyitondekane kumurongo wa macroporus resin (150ml macroporous resin yumye 75g) hamwe ninshuro 1.5 yumubare wibiyobyabwenge, ukuramo 600ml yamazi na 450ml ya 20% Ethanol kugirango ukureho umwanda, hanyuma ushyire hamwe na 600ml ya 50% ya Ethanol, kandi eluent iboneka mukumisha vacuum 40 ℃.

Yang Yonghua n'abandi.Yakoresheje imikorere ya capillary electrophoresis hamwe na chromatografiya ikora cyane kugirango umenye ibiri muri isorhamnetin 232o2, neohesperidin na tifoside muri Puhuang.Hpce-3d ikora cyane capillary electrophoresis igikoresho (Hewlett Packard, USA), quartz capillary inkingi 50 μ m × 56cm (HP), lc-6a ikora cyane ya chromatografiya (sosiyete ya Shimadzu yo mubuyapani), C18 inkingi 5mm × 200mm, ubunini bwa 5 μ M (sosiyete ya Dalian Yite).Imiterere ya HPCE: buffer 0.02mol / l borax-0.05mol / l 10% acetonitrile ya sodium dodecyl sulfate (SDS), voltage 20kV, ubushyuhe bwinkingi 30 ℃, uburebure bwumuraba 270nm, inshinge 30KPa · s.Imiterere ya HPLC: icyiciro kigendanwa cyari amazi ya acetonitrile (15:85), ubushyuhe bwinkingi bwari 25 ℃, nuburebure bwumurongo wa 254 nm.

Tifaneoside Yerekana ibintu

[izina]tifonioside [1]

[CAS No.]104472-68-6

[uburyo bwo gutahura]HPLC ≥ 98%

[Ibisobanuro]20mg 50mg 100mg 500mg 1g (irashobora gupakirwa ukurikije ibyo abakiriya bakeneye)

[imitungo]ifu ya amorphous