Oloa

Typhonine glycoside

O Puhuang o le poleni mago o Typha angustifolia L., Typha Orientalis Presl poʻo laʻau tutusa i le Typhaceae.O le anotusi o le isorhamnetin-3-o-neohesperidin i le Chinese Pharmacopoeia (2005 Edition) e fuafuaina e le HPLC.O auala autu mo le fuafuaina o le isorhamnetin-3-o-neohesperidin i Typhae ma ana sauniuniga e

HPLC.I lalo ole fa'atatauga ole Typha ile Chinese Pharmacopoeia (2005 Edition):

Tulaga o le Chromatographic ma le su'ega o le fa'aogaina o faiga: octadecyl silane fa'apipi'i silica gel e fa'atumu;O le vai acetonitrile (15:85) sa fa'aaogaina e fai ma vaega feavea'i;Ole umi ole galu ole iloa ole 254 nm.Koluma vevela 40 ℃.Ole numera o papatusi faʻapitoa e le tatau ona itiiti ifo i le 1000 e tusa ai ma le pito i luga ole isorhamnetin-3-o-neohesperidin.

Sauniuniga o vaifofo su'esu'e: ave pe tusa ma le 0.5g o lenei oloa, fua sa'o, tu'u i totonu o se fagu fua 50ml, fa'aopoopo le 45ml o le methanol, togafitiga ultrasonic (mana 250W, taimi 20KHz) mo le 30 minute, fa'amafanafana, fa'aopoopo le methanol i le fua, lulu lelei, faamama, ma ave le filtrate faifai pea

Fuafuaga o le isorhamnetin-3-o-neohesperidin i Puhuang ma ana sauniuniga e le HPLC.O le fa'aogaina lautele o le fa'aogaina o le vai acetonitrile:

① Hp1050 maualuga fa'atinoga vai chromatograph;Chromatographic koluma C18 (5) μm,4.6mm × 250mm) Dalian Inisetiute o Chemical Physics, Saina Academy o Saienisi);Vaega feavea'i: vai acetonitrile (15:85);Sailiga umi galu: 254nm;Koluma vevela vevela.

② O le chromatograph vai maualuga o le hp1050 system a le Hewlett Packard Company i le Iunaite Setete;Chromatographic koluma bondclone 10 C18 (3.9mm) × 300mm； Vaega feaveai: vai acetonitrile (80:20);iloa le umi galu 254 nm, bandwidth 4 nm;fa'asinoga galu umi 550 nm, bandwidth 100 nm;fa'aitiitia 8.

Fu Daxu et al.Fuaina mea o loʻo i totonu o typhoniflorin ma isorhamnetin-3-o-neohesperidin i le aofaʻi o le flavone extract a Typha e HPLC ma le 1100 chromatograph.O le koluma chromatographic o le eclipse XdB C18 (250mm × 4.6mm，5 μm); Vaega feavea'i: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15:78:7; 15min: 23:70:7);Ole umi ole galu ole iloa ole 248 nm.Sauniuniga o le aofaʻi o le flavonoids o Typha: 50g o Typha na maua mai i le 60% ethanol (500ml) × 2), 1H i taimi taʻitasi, o le faʻapipiʻi na faʻapipiʻi i le 40 ℃, o le vaega na faʻaumatia i le 600ml vai ma centrifuged.O le toega na fa'amavaeina i le 300ml vai ma fa'a'ese'ese.Faʻatasi le vaifofo centrifugal, faʻapipiʻi le voluma i le 1000ml i le vai, faʻapipiʻi i luga o se koluma o le resini macroporous (150ml macroporous resin mamago mamafa 75g) faʻatasi ma le 1.5 taimi le aofaʻi o fualaau faasaina, elute i le 600ml vai ma le 450ml o le 20% ethanol e aveese. eleelea, ona fa'afefeteina lea i le 600ml o le 50% ethanol, ma o le eluent e maua i le fa'agogo mamago i le 40 ℃.

Yang Yonghua et al.Faʻaaogaina le maualuga o le faʻaogaina o le capillary electrophoresis ma le maualuga o le faʻaogaina o le chromatography vai e fuafua ai mea o loʻo i totonu o le isorhamnetin 232o2, neohesperidin ma typhoside i Puhuang.Hpce-3d maualuga fa'atinoga capillary electrophoresis meafaigaluega (Hewlett Packard, ISA), quartz capillary koluma 50 μm × 56cm (HP), lc-6a maualuga fa'atinoga vai chromatograph (Shimadzu kamupani a Iapani), C18 koluma 5mm × 200mm, vaega laiti 5 μ M (Kamupani Dalian Yite).Tulaga HPCE: buffer 0.02mol/l borax-0.05mol/l 10% acetonitrile o le sodium dodecyl sulfate (SDS), voltage 20kV, koluma vevela 30 ℃, suʻesuʻeina galu umi 270nm, tui voluma 30KPa · s.Tulaga HPLC: o le vaega feaveaʻi o le vai acetonitrile (15:85), o le vevela o le koluma e 25 ℃, ma o le umi o le galu e iloa ai o le 254 nm.

Typhaneoside Reference substance

[igoa]typhonioside [1]

[CAS Nu.]104472-68-6

[auala su'esu'e]HPLC ≥ 98%

[Fa'amatalaga]20mg 50mg 100mg 500mg 1g (e mafai ona afifiina e tusa ai ma manaoga o tagata faatau)

[meatotino]pauta amorphous