Isoorientin;Homoorientin CAS No. 4261-42-1
Ruzivo Rwakakosha
Zita rechiChinese: isolysine
Chirungu Zita: isoorientin
Chirungu alias: homoorientin;(1S) -1,5-anhydro-1-[2-(3,4-dihydroxyphenyl) -5,7-dihydroxy-4-oxo-4H-chromen-6-yl]-D-glucitol
Nhamba yeCAS: 4261-42-1
Molecular Formula: C21H20O11
Molecular uremu: 448.3769
Physicochemical Properties
Kuchena: pamusoro pe99%, nzira yekuona: HPLC.
Density: 1.759g/cm3
Poindi yekubikira: 856.7 ° C pa760 mmHg
Flash point: 303.2 ° C
Steam pressure: 2.9e-31mmhg pa25 ° C
Biological Chiitiko cheIsoorientin
Tsanangudzo:isoorientin inoshanda COX-2 inhibitor ine IC50 kukosha kwe39 μ M.
Zvikamu zvinoenderana:
Research field > > cancer Natural Products > > flavonoids
Tsvagiridzo yemunda > > kuzvimba / kusadzivirirwa
Chinangwa: cox-2:39 μ M (IC50)
In vitro zvidzidzo:Isoorientin is selective inhibitor ye cyclooxygenase-2 (COX-2) kubva mubhubhu yePueraria tuberosa [1].PANC-1 uye patu-8988 masero akabatwa neIsoorientin (0,20,40,80 uye 160 μ M) Kura muhupo hwemaawa makumi maviri nemana uye kuwedzera CCK8 mhinduro.Pa20, 40, 80 uye 160 μ Pamusangano weM, kushandiswa kwesero kwakaderera zvikuru.Isoorientin (0,20,40,80 uye 160) yakashandiswa kumasero μ M yePANC-1;0, 20, 40, 80160 uye 320 μ M yakashandiswa patu-8988) tsika kwemaawa makumi maviri nemana, uye kutaura kweP kwakaongororwa neWestern blot - AMPK uye AMPK.Kutaura kwep-ampk kwakawedzera mushure mekurapa kweIsoorientin.Zvadaro, muboka re shRNA, 80 μ M yekutarisa kuona mhedzisiro yeIsoorientin.Matauriro emazinga e AMPK uye p-ampk muboka re shRNA aive akaderera zvakanyanya pane ayo ari musango-mhando PC maseru (WT) uye boka rakatapurirana neagative control lentivirus (NC) [2].
In vivo zvidzidzo:Mhuka dzairapwa neIsoorientin pa10 mg/kg uye 20 mg/kg uremu hwemuviri hwakava nekuderera kwakakosha kweclaw edema, nehupamhi hwepamusoro hwe1.19 ± 0.05 mm uye 1.08 ± 0.04 mm, zvichiteerana.Izvi zvakaratidza kuti Isoorientin yakanyanya kuderedza paw edema kana ichienzaniswa neboka rekutonga [3].
Sero kuedza:PANC-1 uye patu-8988 masero akaiswa mune 96 matsime mahwendefa.Tsime rega rega rine ~ 5000 maseru uye mazana maviri maseru μ L yepakati ine 10% FBS.Kana maseru mutsime rega rega asvika 70% confluence, iyo yepakati yakashandurwa uye FBS yemahara yepakati ine akasiyana siyana eisoorientin yakawedzerwa.Mushure memaawa makumi maviri nemana, masero akashambidzwa kamwe chete nePBS, tsika yetsika ine isoorientin yakaraswa, uye 100% yakawedzerwa μ L FBS yemahara medium uye 10 μ L cell counting kit 8 (CCK8) reagent.Masero akaiswa pa 37 ℃ kweimwe maawa 1-2, uye kunyura kwetsime rimwe nerimwe kwakaonekwa pa490 nm uchishandisa ELISA muverengi.Kushanda kwesero kunoratidzwa sechinjo yakawanda mukunyura [2].
Kuedza kwemhuka:kana iri paw edema modhi, mbeva [3] dzakapihwa isoorientin kana celecoxib intraperitoneally, uye carrageenan yaibayiwa yakananga mupaw awa imwe gare gare.Mune iyo airbag modhi, marapirwo ese anopinda mubhegi mhango zvakananga necarrageenan.isoorientin yakaiswa 3 maawa carrageenan isati yaiswa mu capsule.Isoorientin uye celecoxib zvakapihwa kune mbeva.Stock solutions ye isoorientin (100 mg / ml) uye celecoxib (100 mg / ml) yakagadzirwa muDMSO uye yakawedzera kuderedzwa panguva yekurapa.Mhuka dzakakamurwa kuva mapoka mashanu anotevera akasiyana: kutonga (DMSO yakarapwa);Yakarapwa carrageenan (0.5 ml (1.5% (w / V) carrageenan mune brine); Yakarapwa carrageenan + celecoxib (20mg / kg uremu hwemuviri); Yakarapwa carrageenan + isoorientin (10 mg / kg uremu hwemuviri); Rapa carrageenan + isoorientin (20mg / kg uremu hwemuviri).
Reference:[1].Sumalatha M, et al.Isoorientin, Selective Inhibitor yeCyclooxygenase-2 (COX-2) kubva kuTubers yePueraria tuberosa.Nat Prod Commun.2015 Oct;10(10):1703-4.
[2].Ye T, nevamwe.Isoorientin induces apoptosis, inoderedza invasiveness, uye inodzika pasi VEGF secretion nekuita AMPK kusaina mupancreatic cancer maseru.Onco Targes Ther.2016 Dec 12;9:7481-7492.
[3].Anilkumar K, nevamwe.Kuongororwa kweAnti-Inflammatory Properties yeIsoorientin Isolated kubva kuTubers yePueraria tuberosa.Oxid Med Cell Longev.2017;2017:5498054.
