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Tsanangudzo:
pachymic acid is a triterpenoid kubva kuP. cocos.Pachymic acid inhibits Akt uye ERK kusaina nzira.

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research field > > cancer
Natural Zvigadzirwa>> terpenoids uye glycosides

Chinangwa:
Akt
ERK

In Vitro Chidzidzo:
pachymic acid (PA) inogona kuvharidzira tumorigenesis yegomarara renduru inosanganisira nzira dzechiratidzo dzeAkt uye ERK.Pachymic acid (PA) kurapwa kwakavharisa zvakanyanya Rho a, Akt uye ERK nzira mumasero egomarara renduru.Pachymic acid (PA) kurapwa inogona kuderedza PCNA, ICAM-1, RhoA, p-Akt uye perk nenzira inoenderana nedosi.Maawa gumi nemaviri mushure mekurapwa, 10 μ G / ml Poria asidhi (PA) inhibited sero kukura pakusangana kwe30 μ G / ml yakawedzera kuderedza kukura kwesero.Kukura kwesero kwakadziviswa munguva - uye nedosi-inotsamira nzira.Mushure memaawa makumi mana nemasere ekurapwa, chiyero chaive 10 μ g/ mL, 20 μ G / ml uye 30 μ Pachymic acid (PA) pa g / ml inhibited sero kukura neinenge 25%, 40% uye 70%.Pachymic acid (PA) inodzivirirawo kukura kwemasero egomarara renduru nenzira inotsamira uye inotsamira mudosi [1].

MuVivo Chidzidzo:
kuitira kuti uongorore maitiro e-antitumor epachymic acid (PA) mu vivo, kenza yemapapu emunhu nci-h23 tumor xenograft modhi yakashandiswa.Pachymic acid (PA) yakanyanya kuvharidzira bundu kukura pamadosi e30 uye 60 mg / kg kwemazuva 21 zvichienzaniswa neboka rekutonga [2].

Cell Experiment:
cell counting kit-8 (CCK-8) yakashandiswa kuongorora anti proliferation effect ye pachymic acid (PA) paGBC-SD masero.Muchidimbu, mushure mekurapa kwakatarwa, wedzera gumi kune rimwe gomba μ Lcck-8 mhinduro, uye mushure meimwe incubation, kunyura kwakayerwa pa450 nm uchishandisa microplate reader [1].

Kuedza kwemhuka:
mbeva [2] vakashandisa vakadzi athymic nude mbeva vane makore 4-5 mavhiki.nci-h23 masero akakura zvakanyanya (pa100 μ 5 muLPBs × 106) akaiswa pasi pecutaneously mudivi rekurudyi rembeva yega yega.Tumor xenografts yakakura kusvika paavhareji saizi ye100-200mm 3 uye yakasarudzika kumapoka mana ekurapa akasiyana (6 mice muboka rimwe nerimwe): (a) kutonga kwemotokari (0.1% DMSO mune yakajairika saline);(b) Pachymic acid (PA) 10 mg / kg;(c) PA 30 mg / kg;(Dd) PA 60mg / kg. Mice dzakapiwa pa ne intraperitoneal (IP) jekiseni kwemavhiki matatu (5 mazuva / vhiki).Saizi yebundu yakayerwa pamasanhu maviri nerubatsiro rwevernier caliper, uye bundu vhoriyamu (mm3) yakaverengerwa.

Reference:
[1].Chen Y, nevamwe.Pachymic acid inhibits tumorigenesis mu gallbladder carcinoma masero.Int J Clin Exp Med.2015 Oct 15;8(10):17781-8.
[2].MaJ, nevamwe.Pachymic acid induces apoptosis kuburikidza ne activating ROS-inotsamira JNK uye ER kushushikana nzira mumaseru ekenza yemapapu.Cancer Cell Int.2015 Aug 5;15:78.