Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
Ruzivo Rwakakosha
Salvianolic acid B ndiko kukwenenzverwa kwemamorekuru matatu eDanshensu uye imwe molecule yecaffeic acid.Ndiyo imwe yeakanyanya kudzidzwa salvianolic acids.Iyo ine yakakosha pharmacological mhedzisiro pamwoyo, uropi, chiropa, itsvo uye dzimwe nhengo.Ichi chigadzirwa chine mhedzisiro yekusimudzira kutenderera kweropa uye kubvisa ropa stasis, dredging meridians uye activate collaterals.Inonyanya kushandiswa kurapa ischemic sitiroko inokonzerwa neropa stasis inovharira meridians, senge chiveve chehafu yemuviri nemakumbo, kushaya simba, kurwadziwa kwechibvumirano, kutadza kwemota, muromo uye kutsauka kweziso, nezvimwe.
Zita rezita:salvianolic acid B, salvianolic acid B, salvianolic acid B
Chirungu Zita:salvianolic acid B
Molecular formula:c36h30o16
Molecular uremu:718.62
Nhamba yeCAS:115939-25-8
Nzira yekuona:HPLC ≥ 98%
Zvinodiwa:10mg, 20mg, 100mg, 500mg, 1g (inogona kuiswa mukati maererano nezvinodiwa nevatengi)
Basa uye kushandiswa:Ichi chigadzirwa chinoshandiswa kugadzirisa zvirimo.
Zvenyama uye zvemakemikari zvinhu
Properties:chigadzirwa chacho quasi white powder.
Kuravira kunovava zvishoma uye kunonatsa, nechinhu chinoita kuti mwando.Soluble mumvura, ethanol uye methanol.
Salvianolic acid B inoumbwa nekudzikamisa mamorekuru matatu e salvianolic acid uye 1 molecule yecaffeic acid.Iine mapoka maviri e-carboxyl uye iripo mumhando yemunyu wakasiyana (K +, Ca2 +, Na +, NH4 +, nezvimwewo).Mukuita kweDecoction uye kuisa pfungwa, chikamu chiduku che salvianolic acid B chinogadziriswa kusvika kune yepepuru oxalic acid uye salvianolic acid, uye chikamu che salvianolic acid B chinova rosmarinic acid pasi pemamiriro eacidic;Salvianolic acid A uye C inogona kuve tautomeric mumhinduro.
Zvinotsanangurwa
＞5%,＞10%,＞50%,＞70%,＞90%,98%
Extraction Process
Iyo Radix Salviae Miltiorrhizae yakapwanyika, ikaiswa mutangi rekuchera, yakanyoroveswa ne8 yakapetwa huwandu hwe0.01mol/l hydrochloric acid usiku humwe, uyezve yakanyungudika nekagumi neina kuwanda kwemvura.Iyo percolated yakabviswa mhinduro inonatswa neAB-8 macroporous resin column.Chekutanga, elute ne 0.01mol/l hydrochloric acid kubvisa tsvina isina adsorbed, uyezve elute ne 25% ethanol kubvisa zvakanyanya polar tsvina.Chekupedzisira, simbisa iyo 40% ethanol eluent pasi pekumanikidzwa kwakaderedzwa kudzosa ethanol uye kuomesa-kuoma kuti uwane yakazara Salvia miltiorrhiza phenolic acid nekuchena kunodarika 80%.
Ziva
Tora 1g yechigadzirwa, igaye, wedzera 5ml ye ethanol, nyatso kurunga, sefa, tora madonhwe mashoma efiltrate, nyora papepa resefa strip, iomese, ione pasi pe ultraviolet rambi (365nm), ratidza bhuruu- grey fluorescence, sungirira bepa resefa muyakaomeswa ammonia solution bhodhoro (isingabatanidzi mvura yepamusoro), ibudise mushure memaminitsi makumi maviri, ione pasi pe ultraviolet rambi (365nm), ratidza bhuruu-girinhi fluorescence.
Acidity:tora mhinduro ine aqueous pasi pechinhu chekujekesa, uye pH kukosha ichave 2.0 ~ 4.0 (appendix yeChinese Pharmacopoeia 1977 Edition).
Content Determination
Yakatemerwa nepamusoro pekuita liquid chromatography (Appendix VI D, Vhoriyamu I, Chinese Pharmacopoeia, 2000 EDITION).
Octadecyl silane bonded silica gel yakashandiswa sekuzadza mune chromatographic mamiriro uye system applicability bvunzo;Methanol acetonitrile formic acid water (30:10:1:59) ndiyo yaive mobile phase;Iyo yekuona wavelength yaive 286 nm.Huwandu hwetioretical mahwendefa akaverengerwa zvinoenderana nesalvianolic acid B peak haifanire kunge iri pasi pe2000.
Kugadzirira kwereferensi mhinduro kuyera huwandu hwakakodzera hwesalvianolic acid B referenzi yemhinduro uye wedzera mvura kuti iite ine 10% pa 1ml μ G mhinduro.
Kugadzirira kwebvunzo mhinduro tora inenge 0.2g yechigadzirwa, kunyatsoyera uremu, isa mubhodhoro rekuyeresa 50ml, wedzera huwandu hwakakodzera hwemethanol, sonicate kwemaminitsi makumi maviri, itonhodze, wedzera mvura pachikero, zunza zvakanaka, sefa. iyo, kunyatsoyera 1ml yefiltrate inopfuurira, isa mubhodhoro rekuyeresa 25ml, wedzera mvura kuchiyero, zunza zvakanaka.
Nzira yekutsunga inonyatso tora 20% yemhinduro yekugadzirisa uye 20% yebvunzo mhinduro μ l.Ijekiseni mukati mechromatograph yemvura kuti zvionekwe.
Pharmacological Efficacy
Salvianolic acid B ndiko kukwenenzverwa kwemamorekuru matatu eDanshensu uye imwe molecule yecaffeic acid.Ndiyo imwe yeakanyanya kudzidzwa salvianolic acids.Iyo ine yakakosha pharmacological mhedzisiro pamwoyo, uropi, chiropa, itsvo uye dzimwe nhengo.
Antioxidant
Salvianolic acid B ine yakasimba antioxidant mhedzisiro.Kuedza mu vivo uye in vitro kunoratidza kuti salvianolic acid B inogona kuchenesa okisijeni isina radicals uye inhibit lipid peroxidation.Kusimba kwayo kwekuita kwakakwira kupfuura kwevhitamini C, vhitamini E uye mannitol.Icho chimwe chezvinhu zvinozivikanwa zvechisikigo zvigadzirwa zvine yakasimba antioxidant mhedzisiro Zvidzidzo zvePharmacological zvinoratidza kuti salvianolic acid yejekiseni ine pachena antioxidant effect, inhibits platelet aggregation uye thrombosis, uye inogona kurebesa nguva yekupona kwemhuka pasi pe hypoxia.Zvigumisiro zvakaratidza kuti salvianolic acid yejekiseni (60 ~ 15mg / kg) inogona kuvandudza zvakanyanya kukanganisa kwetsinga mumakonzo ane cerebral ischemia-reperfusion kukuvara, kuvandudza maitiro ehutano uye kuderedza zvakanyanya nzvimbo yecerebral infarction.Pakave nemusiyano wakakura pakati peakakwira uye epakati madosi (60 uye 30mg / kg);Salvianolic acid yejekiseni inogona kuvandudza zvakanyanya kukanganisa kweurological kunokonzerwa neFeCl3 induced cerebral ischemia mumakonzo pa1, 2 uye maawa 24 mushure mekutonga, iyo inoratidzwa mukuvandudzwa kwehutano hwehutano uye kuderedzwa kwecerebral infarction nzvimbo;Salvianolic acid 40 mg / kg yejekiseni yakanyanya kurambidza kuunganidzwa kwetsuro maplatelet anokonzerwa neADP, arachidonic acid uye collagen, uye inhibition rates yaive 81.5%, 76.7% uye 68.9% zvichiteerana.Salvianolic acid 60 uye 30mg / kg yejekiseni inodzivirira zvakanyanya thrombosis mumakonzo;Salvianolic acid 60 uye 30mg / kg yejekiseni yakawedzera zvakanyanya nguva yekupona yemakonzo pasi pe hypoxia.
Clinical Application
Ichi chigadzirwa chine mhedzisiro yekusimudzira kutenderera kweropa uye kubvisa ropa stasis, dredging meridians uye activate collaterals.Inonyanya kushandiswa kurapa ischemic sitiroko inokonzerwa neropa stasis inovharira meridians, senge chiveve chehafu yemuviri nemakumbo, kushaya simba, kurwadziwa kwechibvumirano, kutadza kwemota, muromo uye kutsauka kweziso, nezvimwe.
Store
Munzvimbo inotonhorera uye yakaoma.
Term of Validity
Makore maviri.
Kuchengeta Nzira
2-8 ° C, yakachengetwa munzvimbo inotonhorera uye yakaoma uye kure nechiedza.
Nyaya Dzinoda Kufungwa
Chigadzirwa chacho chinofanira kuchengetwa pakupisa kwakadzika.Kana ikaratidzwa kumhepo kwenguva yakareba, zvinyorwa zvichaderedzwa.
