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Chirungu Zita:tanshinone IIA
Chine zita reChinese:tanshinone |tanshinone IIA |tanshinone 2A |tanshinone IIA |tanshinone IIA biological chiitiko

Tsanangudzo:
Tanshinone IIA (Tan IIA) ndechimwe chezvinyorwa zvinonyungudika nemafuta mumidzi yemudzi mutsvuku Salvia miltiorrhiza.Tanshinone IIA inogona kuvharidzira angiogenesis nekutarisa protein kinase domain yeVEGF / VEGFR2.

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Chinangwa:
VEGF/VEGFR2[1]

In Vitro Chidzidzo:Iyo antitumor mhedzisiro ye tanshinone IIA inosanganisira inhibiting tumor cell kuwanda, kukanganisa tumor cell cycle, kukurudzira tumor cell apoptosis uye inhibiting tumor cell invasion uye metastasis.Tanshinone IIA yakanga ine anti proliferative effect pamasero eA549: IC50 ye tanshinone IIA mushure me24, 48 uye 72 maawa aiva 145.3, 30.95 uye 11.49, maererano μ M. CCK-8 assay yakashandiswa kuongorora tanshinone IIA μ80 μ-25 (25). Basa rekuwedzera reakarapwa A549 masero pa24, 48 uye 72 maawa, zvichiteerana.CCK-8 mhedzisiro yakaratidza kuti tanshinone IIA inogona kumisa zvakanyanya kupararira kwemasero eA549 nenzira inotsamira uye inotsamira nguva.Yakakosha apoptosis uye sero kukura inhibition yeA549 masero akaonekwa maawa makumi mana nemasere mushure mekurapwa kwezvinodhaka (iyo yekusungwa yakashandiswa yaive yeIC50 kukosha: tanshinone iia31 paA549) μ M) . μ M), pasi gadzirisa kutaura kweVEGF uye VEGFR2 protein muboka rekurapa zvinodhaka uye vector [1].Tanshinone IIA chimwe chezvakanyanya kuwanda zvikamu zveSalvia miltiorrhiza midzi, inogona kuchengetedza masero eH9c2 kubva kuapoptosis.H9c2 masero akabatwa ne tanshinone IIA inhibited angiotensin II induced apoptosis nekudzika kudzora kutaura kwePTEN (phosphatase uye tensin homologue).PTEN ibundu suppressor inoita basa rakakosha muapoptosis.Tanshinone IIA inhibits angiotensin II (AngII) - induced apoptosis nekudzika kudzora kutaura kwephosphatase uye tensin homologue (PTEN) [2].Tanshinone IIA inoderedza mapuroteni kutaura kweEGFR, uye IGFR inovhara iyo PI3K / Akt / mTOR nzira mugastric cancer AGS masero [3].

Cell Experiment:A549 maseru akaverengerwa muchikamu chelogarithmic uye 6000 masero (90 μ L vhoriyamu) mu96 tsime ndiro.Will 10 μ L yakasiyana-siyana ye tanshinone IIA (yekupedzisira concentrations 80,60,40,30,20,15,10,5 uye 2.5 μ M) Uye ADM (yekupedzisira concentrations 8,4,2,1,0.5 uye 0.25 μ M ) Yakawedzerwa kuboka rezvinodhaka, nepo boka rekutonga risina kunaka (boka rinotakura) rakawedzerwa chete 10 μ Ldmso kana saline yakajairika isina tanshinone IIA kana Adm. Sanganisa masero neCCK-8 reagent (100 μ L / ml yepakati) ye imwe 2 maawa, uye absorbance yakaverengwa pa450 nm uchishandisa microplate reader.Kuwedzera kwesero inhibition rate inotarwa maererano neshoko rinotevera: kupararira kwekudzivirira kwehuwandu (%) = 1 - [(A1-A4) / (A2-A3)] × 100, apo A1 ndiyo inokosha yeOD yeboka rekuedza zvinodhaka, A2 ndiyo inokosha yeOD yeboka risina chinhu, A3 ndiyo OD inokosha ye RPMI1640 yepakati isina masero, uye A4 ndiyo OD yemishonga ine huwandu hwakafanana neA1 asi isina masero.Iko IC50 kukosha kwakaverengerwa nenonlinear regression analysis uchishandisa graphpad prism software [1], iyo inomiririra kusanganiswa kwezvinodhaka kunoratidza 50% cell growth inhibition.

Reference:[1].Xie J, nevamwe.Iyo antitumor mhedzisiro ye tanshinone IIA pane anti-kupararira uye kudzikira VEGF/VEGFR2 kutaura pamunhu asiri diki cell kenza yemapapu A549 cell line.Acta Pharm Sin B. 2015 Nov;5(6):554-63.
[2].Zhang Z, nevamwe.Tanshinone IIA inhibits apoptosis mumyocardium nekuita microRNA-152-3p kutaura uye nokudaro kuderedza PTEN.Ndiri J Transl Res.2016 Jul 15;8(7):3124-32.
[3].Su CC, nevamwe.Tanshinone IIA inoderedza kuratidzwa kweprotein yeEGFR, uye IGFR ichivharira PI3K/Akt/mTOR nzira mugastric carcinoma AGS masero zvese mu vitro uye mu vivo.Oncol Rep. 2016 Aug;36(2):1173-9.