Glycyrrhizin, Liquiritin;Liquiritoside;Likviritin;Liquiritoside Cas No.551-15-5
Hordhac Kooban
Glycyrrhizin, sidoo kale loo yaqaan Liquiritin.Licorice waa geedka Glycyrrhiza ee Leguminosae.Xididdada iyo afkoda waa geedo Chinese ah oo caadi ah.
Daawooyinka ayaa si weyn loogu qaybiyey Waqooyi Bari Shiinaha, Xinjiang, Yunnan, Mongolia gudaha, Anhui iyo meelo kale.Shennong materia medica classic ayaa ku taxaysa heerka ugu sarreeya, isagoo leh "cawskani waa boqorka dawooyinka oo dhan, waxaana jira dad yar oo aan isticmaalin".Licorice wuxuu leeyahay qaybo isku dhafan, oo ay ku jiraan triterpenoids, flavonoids iyo coumarins.Flavonoids waa nooc ka mid ah qaybaha bioactive ee laga helay soosaarkii licorice.Qaybaha ay ka kooban tahay kiimikooyinka daawada inta badan waxaa ka mid ah glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, iwm. Sanadihii la soo dhaafay, waxaa jiray warbixino ku saabsan xoqidda xagjirka xorta ah, antioxidant, anti-kansarka iyo saamaynta mutagenic ee flavonoids licorice gudaha iyo dibaddaba.
KiimikoNaamiin:4H-1-Benzopyran-4-kow, 2- [4- (β-D-glucopyranosyloxy) phenyl] -2, 3-dihydro-7-hydroxy-, (S)
Pxamaasad lehPhantidaMonohydrate (milan ethanol ama biyo), barta dhalaalaysa: 212 ~ 213 ° ℃.
Falka dawooyinka
Sunta: Maya
Falcelinta xun: aan la garanayn
Ilaha ka kooban: legume Glycyrrhiza glabra L. xidid, Glycyrrhiza uralensis Fisch Root.
Soo saarista Glycyrrhizin
Ka Hortagga Qalabka Ceyriinka Licorice
Halabuurka kiimikada ee alaabta ceeriin ee licorice waa mid aad u adag.Si loo helo saameyn wanaagsan oo kala-soocid ah, la yareeyo wasakhda wasakhda ah ee tiirka chromatographic diyaarinta, iyo wanaajinta maadada glycyrrhizin ee duridda alaabta ceeriin, habka soo saarista ayaa loo isticmaalay in lagu sii daayo alaabta ceeriin.Ku miisaan cawska 4G oo leh dheelitir elektaroonig ah oo geli digsiga.Si sax ah ugu cabbir 100ml oo biyo nadiif ah oo leh dhululubo cabbir ah oo ku shub digsiga si uu u milmo.Ultrasonic ilaa 15 daqiiqo, oo si joogto ah u walaaq ul galaas ah si loo dedejiyo kala diri.Ka dibna ku rid weelka biyaha qubeyska 90 ℃ oo kuleyl 2 saacadood, ka dibna diiri si aad u nadiifiso.Ka dib marka lagu daro shaandhaynta dareeraha n-butanol oo taagan dhowr daqiiqadood, inta badan glycosides-yada waxaa lagu milmaa n-butanol dareeraha, ka dibna samee soo saarista labaad, soo saar qadar yar oo glycosides ah oo ku haray biyaha, ugu dambeyntiina isku dar oo xooga saar n- xal butanol ah oo lagu helay soosaar labaad oo loogu talagalay chromatography iyo nadiifinta.
Sifaynta Glycyrrhizin By Chromatography
Qaado 10 ml oo ka mid ah alaabta kor ku xusan sida alaabta ceeriin ee firaaqada ah, billow bamka, deji heerka socodka 25 ml / min, oo alaabta ceeriin u geli 500 mm wajiga wareega (methanol: biyo = 1: 4) × In a Tiirka diyaarinta 40 mm, soo ururi jajabka alaabta glucoside hay si waafaqsan xaaladda ugu sarreysa: jajabka 1 h ee ugu horreeya waxaa la soo ururiyaa sida jajabka hore ee wasakhda, ka dibna beddel socodka.Tusaale ahaan, tiirka ku maydh isku-dar ah 50% methanol iyo biyo, ku xidh sheyga 20 kii daqiqiba kasta,kadibna ku ururi dhalo kasta oo badeecad ah oo leh uumi wareeg ah, oo qaado 20 µL oo ah falanqaynta chromatographic HPLC, Ilaa aan la ogaan bartilmaameed.Xaaladaha lagu ogaanayo HPLC waxay ahaayeen sida soo socota: wajiga wareega: methanol: biyaha = 3.5: 6.5;Marxaladda taagan: silica gel carbon 18;Tiirka Chromatographic: 450 mm × 4.6 mm; Heerka socodka: 1 ml / min;Dhererka mowjadda ogaanshaha: 254nm.Waxyaabaha ku jira glycyrrhizin ee dhalada labaad ayaa ah tan ugu sareysa ee alaabta la helo 20 daqiiqo kasta
Nadiifinta Glycyrrhizin ee Rechromatography
Maadaama maadada glycyrrhizin ka dib nadiifinta chromatographic aasaasiga ah aysan sarreyn, hab isku mid ah ayaa la doortaa.Qaado 10 ml oo ah sheyga sare ee la safeeyey sida alaabta ceeriin ee heeganka ah, heerka qulqulka waa 25 ml / min, oo dhalada labaad ee badeecada u keen 500 mm wajiga wareega (methanol: biyo = 2: 5) × 20 mm } tiirka chromatographic, ururi distillate ee alaabta glycoside hay iyadoo loo eegayo xaaladda ugu sarreysa: ku xidh alaabta 4 kasta, ka dibna ku ururi dhalo kasta oo badeecad ah oo leh uumi wareeg ah, oo isticmaal isla xariijimaha ogaanshaha ee sare ee falanqaynta chromatographic HPLC ilaa inta aanay jirin bartilmaameed. .Falanqaynta ka dib, waxaa la ogaaday in maadada glycyrrhizin ee dhalada lixaad ay tahay tan ugu sareysa ee alaabooyinka la helo 4 kasta, taas oo wakhtiga haynta uu ahaa 5.898 min sida ugu sarreysa bartilmaameedka, iyo nuxurku wuxuu gaaray ilaa 40% habka caadiga ah ee aagga. .
Ka Dib Daawaynta Alaabta
Alaabta la soo ururiyey waxaa lagu diiriyaa cadaadis la dhimay oo saaran uumiga wareega ee 70 ℃.Ka dib markii dareeraha asal ahaan uumibaxo, milaan badeecada adag ee ku jirta weelka hoose ee wareegsan oo leh qadar yar oo methanol ah, oo ku shub tuubo tijaabo ah heerkulka qolka ilaa ay ka soo baxaan kiristaalo granular ah [2].
