Alaabta

Bartilmaameedka:JNK1 jnk2 NF- κ B

Barashada vitro:isovitexin waxay ka hortagtaa dhaawaca oksaydhiyaha ee LPS iyadoo la joojinayo soo saarista ROS intracellular, sidoo kale waxay daciifisaa saamaynta H2O2 ee jiritaanka unugyada.Waxa ku jira LPS (2 μ Isovitexin (0-100 g / ml) μG/ml) may lahayn cytotoxicity unug 264.7 ceeriin ah, laakiin 200 μ G/ml isovitexin ayaa muujisay cytotoxicity muhiim ah.Isovitexin (25,50) μ G / ml) waxay joojisay LPS TNF-α, Heerarka kordhay ee IL-6, iNOS iyo COX-2.Isovitexin (25,50) μ G / ml) sidoo kale waxay joojisay I ee unugyada 264.7 cayriin κ B α Fosfooraska iyo hoos u dhaca, taas oo la socota saameynta JNK1 / 2 inhibitor [1].

In vivo waxbarashada:isovitexin (50 iyo 100 mg/kg/kg, IP) waxay sababtay isbeddello aad u daran oo histopathological ah oo ku yimaadda qaybaha sambabada waxayna hoos u dhigtay tirada unugyada bararka ee jiirarka ay keentay LPS.Heteroeosinophils (50 iyo 100 mg / kg, IP) waxay hoos u dhigtay TNF by- α Iyo IL-6 wax soo saarka, wax soo saarka ROS, MPO iyo MDA content, kordhay SOD iyo GSH, oo ka hortagay LPS barar iyo walbahaarka oksidative ee LPS ay keentay Ali jiirarka.Oo si wax ku ool ah u joojiso muujinta borotiinka ee iNOS iyo COX-2 [1].Isovitexin (25,50, 100 mg / kg) waxay hoos u dhigtay heerka badbaadada ee LPS / D-gal ee dhaawaca beerka ee jiirarka iyadoo loo eegayo qiyaasta qiyaasta.Isovitexin sidoo kale waxay joojisay NF-κ B iyo kor u habeynta LPS / D-gal Nrf2 iyo HO-1 ee jiirarka [2].

Tijaabada unugga:jiritaanka unugga waxaa go'aamiyey MTT assay.Unugyada 264.7 ee ceeriin ayaa lagu tallaalay 96 saxan oo ceel ah (1) × 104 unug / ceel) iyo tirooyin kala duwan oo isovitexin ah (fiirsashada kama dambaysta ah: 0-200) μ G / ml) iyo LPS (2 μ G / ml) 24 saacadood.Intaa waxaa dheer, isticmaalka IV (25 ama 50 μG / ml) ayaa horay u daaweeyay unugyada 1 saac, ka dibna H 2O 2 (300) ayaa lagu daray μM) 24 saacadood ka dib, MTT (5 mg / ml) ayaa lagu daray unugyo ka dibna lagu beeray 4 saacadood [1].

Tijaabada xayawaanka:jiirarka [1] si loo dhiso model Ali, jiirarka ayaa si aan kala sooc lahayn loo qaybiyay 6 kooxood: kantaroolka (saline), kaliya isovitexin (100 mg / kg, lagu milmay 0.5% DMSO), kaliya LPS (0.5 mg / kg, lagu milmay saline). LPS (0.5 mg / kg) + isovitexin (50 ama 100 mg / kg) iyo LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, oo lagu milmay saline).Isovitexin ama DEX (5 mg / kg) isovitexin ayaa la maamulay.Ka dib soo-gaadhista isovitexin ama DEX muddo 1 saac ah, jiirarka ayaa lagu suuxiyay ether waxaana LPS lagu maamulay gudaha sanbabada si loo keeno dhaawaca sambabada.Xayawaankan ayaa la nadiifiyey 12 saacadood kadib maamulka LPS.Sidaa darteed, dheecaanka dheecaanka bronchoalveolar (BALF) iyo shaybaarada unugyada sambabada ayaa la goostay si loo cabbiro heerarka cytokine;Jiilka ROS;SOD, GSH, MDA iyo hawlaha MPO;Iyo muujinta COX-2, iNOS, HO-1 iyo borotiinnada Nrf2 [1].