Alaabta

Bartilmaameedka:GABA reseptor, mTOR, PI3K, Akt [1] [2]

Barashada vitro:qiyaas yar 41 μ Jujuboside A of M (qiyaastii 0.05 g / L) GABA (a) reseptors inta lagu jiro 24 iyo 72 h daawaynta α 1, α 5, β 2 subunit mRNA si weyn u kordhay.Qiyaasta sare ee 82 μM (qiyaastii 0.1 g / L) Jujuboside A ayaa si weyn u kordhay GABA (a) reseptors 24 h α1, α 5 heerarka mRNA iyo hoos u dhaca β 2 heerka mRNA iyo hoos u GABA (a) receptor subunit α 1, β2 mRNA muujinta waxaa lagu daaweeyay 72 h [1].Jujuboside Daawaynta ka horraysa waxay dib u celin kartaa hoos u dhaca jiritaanka unugyada waxayna si fiican u keeni kartaa ISO dhaawaca unugyada H9c2.Jujuboside A waxay dedejin kartaa fosforyaalka PI3K, Akt iyo mTOR.Jujuboside A waxay si weyn u yarayn kartaa saamiga borotiinka microtubule ee la xidhiidha LC3-II / I ee unugyada H9c2 [2]

In vivo waxbarashada:maalintii (9:00-15:00), jujubosides waxay si wayn u kordhisay hurdo dhan hurdo iyo dhaq-dhaqaaq isha oo degdeg ah (REM), laakiin wax saamayn ah kuma yeelan hurdo la'aanta REM (NREM).Habeenkii (21: 00-3: 00), jujubosides waxay si weyn u kordhiyeen hurdo dhan hurdo iyo hurdo NREM, gaar ahaan hurdo fudud, laakiin wax saameyn ah kuma yeelan hurdada REM iyo hurdo gaaban oo hurdo ah (SWS) [3].Daawaynta intraventricular ee jjuboside a waxaa si weyn u yareeyey a dhex Y-maze, ka fogaansho firfircoon iyo cabbirada moris biyaha Morris β 1-42 keentay waxbarashada iyo xusuusta liidata ee jiirarka.Cirridka intracerebroventricular ee Jujuboside A hoos u dhaca hippocampal a β 1-42 heerarka ayaa si weyn u hakiyay hawlaha acetylcholinesterase (AChE) iyo maya, oo hoos u dhigay content korodhka malondialdehyde (MDA) ee hippocampus iyo kiliyaha cerebral ee jiirarka lagu duray lateral ventricle β 1-42 [4]

Tijaabada unugga:7 maalmood ka dib dhaqameedka in vitro, unugyadu waxay si joogto ah u soo shaac baxeen 24 saacadood ama 72 saacadood, oo aan loo bandhigin dhexdhexaad ka kooban Jujuboside A ama diazepam ama labadaba.Vector ayaa lagu daray kooxda xakamaynta;Kooxda Diazepam waxay ku dareen 10 μM diazepam;Jujuboside A82 μM (qiyaastii 0.1g / L) iyo 41 μM (qiyaastii 0.05 g / L) ayaa lagu daray jua-h (qiyaas sare oo Jujuboside A) iyo jua-l (qiyaasta Jujuboside A) kooxaha, siday u kala horreeyaan.Wadarta guud ee RNA ayaa laga soocay unugyada si loo baaro [1].

Tijaabada xayawaanka:jiirarka: a waxaa lagu duray iyada oo loo marayo ICV β 1-42 ay keentay daciifnimada garashada ee jiirarka.Ka dib, jiirarka ayaa lagu duray Jujuboside A (0.02 iyo 0.2 mg / kg) intraventricular (ICV) muddo 5 maalmood ah.Maze Y, ka fogaansho firfircoon iyo tijaabooyinka waallida biyaha Morris ayaa lagu sameeyay jiirarka [1].