Alaabta

Sharaxaad:
prim-o-glucosylcimifugin waxay leedahay saameyn xoog leh oo ka hortagga bararka.Muujinta iNOS iyo COX-2 waa la joojin karaa iyadoo la xakameynayo calaamadaha JAK2 / STAT3

Qaybaha khuseeya:
Jidka calaamadaynta > > difaaca iyo caabuqa > > nitric oxide synthase
Goobta cilmi-baarista > > caabuq / difaac
Alaabta Dabiiciga ah > > kuwa kale

Bartilmaameedka:iNOS
COX-2

Barashada Vitro dhexdeeda:
prim-o-glucosylglycine (POG) waa waxa ugu sarreeya ee chromone iyo mid ka mid ah qaybaha ugu muhiimsan ee firfircoon ee Fangfeng (RS).Prim-o-glucosylglycine wuxuu ka ciyaaraa door ka-hortagga caabuqa ee 264.7 macrophages cayriin iyadoo la xakameynayo JAK2 / STAT3 gudbinta calaamadaha iyo joojinta muujinta iNOS iyo COX-2.Sitotoxicity ee prim-o-glucoside ee LPS ee firfircoon 264.7 macrophages cayriin ayaa la qiyaasay.Isticmaal LPS (1 μG / ml) iyo kordhinta uruurinta prim-o-glucosylglycine (15, 50 iyo 100 μG / ml) 24 saacadood, iyo jiritaanka unugyada waxaa qiimeeyay CCK-8 assay.Marka la barbar dhigo unugyada DMSO la daweeyay (xakamaynta), 24 saacadood iyo soo-gaadhista 15-100 μ Ka dib g/ml prim-o-glucoside, jiritaanka unugga si weyn looma saameynin.Si loo barto saameynta anti-bararka ee prim-o-glucosylglycine, haddii prim-o-glucosylglycine ay saameyn karto NO synthesis ayaa lagu baaray unugyada LPS firfircoon ee 264.7 ceeriin.Isticmaal LPS (1 μG / ml) iyo xaddi kala duwan oo prim-o-glucosylglycine ah (15, 50 iyo 100 μG / ml) 24 saacadood.U fiirsashada laguma cabbirin dhaqanka sare ee falcelinta Griess.Isku-duubnida maya ee dhaqameedku waxay si aad ah u korodhay soo-gaadhista LPS, iyo prim-o-glucoside waxay si weyn u joojisay LPS wax-soo-saar ku-tiirsane [1].
In Vivo Daraasadda
Dheecaanka fayadhowrka bronchoalveolar (BALF) ayaa la ururiyay 7 saacadood ka dib maamulka lipopolysaccharide (LPS), iyo heerka cytokine ee BALF waxaa lagu qiyaasay ELISA.Marka la barbardhigo kooxda kantaroolka, TNF ee BALF- α, IL-1 β Iyo IL-6 heerarka ayaa si aad ah u kordhay.Si kastaba ha noqotee, ka-hortagga pre-o-glucosylglycine (2.5, 5 ama 10 mg / kg) ayaa si weyn hoos ugu dhigay TNF-α, IL-1 β Iyo IL-6 qaab ku-tiirsanaan (P <0.05, P <0.05, 0.01) ) [1].

Tijaabada Unugyada
Qalabka tirinta unugyada (CCK-8) ayaa loo adeegsaday si loo go'aamiyo fiirsashada cytotoxic ee prim-o-glucosylglycine.Marka la soo koobo, 264.7 unug oo ceeriin ah ayaa lagu daweeyey 1 ceelkiiba × Cufnaanta 104 unug ayaa lagu tallaalay 96 ceel waxaana la galiyey hal habeen.Ka dib isticmaal 1 μ Unugyada waxaa lagu kiciyey g/ml LPS waxaana lagu daaweeyey xaddi kala duwan oo prim-o-glucosylglycine ah (15, 50 iyo 100 μg/ml; Medchem express, Princeton, NJ, USA) ama daawaynta DMSO.Ka dib markii la kariyey at 37 ℃ 24 saacadood, xal CCK-8 ayaa lagu daray ceel kasta oo ku shubay saacad kale.Nuugista waxaa lagu cabbiray 450 nm iyadoo la adeegsanayo akhristaha microplate [1].

Tijaabada Xoolaha
jiirar [1] BALB / C jiirar lab ah, 8 toddobaad jir ah, oo miisaankoodu yahay 18 ilaa 20g.Jiirarka ayaa si aan kala sooc lahayn loo qaybiyay 5 kooxood: kooxda xakamaynta;Kooxda LPS;LPS + prime-o-glucosylglycine (2.5, 5 ama 10mg / kg miisaanka jirka).Prime-o-glucosylglycine waxaa lagu maamulay intraperitoneal.1 saac ka dib, jiirarka kooxda LPS iyo kooxda LPS + prime-o-glucosylglycine ayaa la siiyay 50 mg / L intranasal (in) (200 mg / L) μ LLPs si loo keeno dhaawac sambabada ba'an.Jiirarka kantaroolka waxaa la siiyay 50% sanka gudahiisa (gudaha) iyada oo aan LPS μ LPBS
[1]

Tixraac:
[1]Zhou J, iyo al.Prim-O-glucosylcimifugin Attenuates Lipopolysaccharide waxay keentay Jawaabta caabuqa ee RAW 264.7 Macrophages.Farmashiyaha Mag.2017 Jul-Sep;13(51):378-384.
[2].Chen N, iyo al.Prime-O-glucosylcimifugin waxa uu yareeyaa dhaawaca ba'an ee sambabada ee lipopolysaccharide.Int Immunopharmacol.2013 Jun;16(2):139-47.