Alaabta

Diyaarinta xalka tixraaca:Qaado qadarka saxda ah ee xalinta tixraaca ee Ruscogenin, si sax ah u miisaam, oo ku dar methanol si aad uga dhigto 50% halkii 1 ml μG. ml ee xalka tixraaca, ku rid weel koofiyad leh oo leh meel istaage siday u kala horreeyaan, oo ka soo bax dareeraha biyaha qubeyska.Si sax ah ugu dar 10ml perchloric acid, si fiican u rux, ku hay biyo kulul 15 daqiiqo, ka saar, ku qabooji biyo baraf ah, qaado reagent u dhigma sida maran, cabbir nuugista mawjada dhererka 397nm marka loo eego ultraviolet spectrophotometry muuqda ( Lifaaqa VA), u qaado nuugista sida ordinate iyo xoogga saarista sida abcissa, oo sawir qalooca caadiga ah.

Diyaarinta xalka tijaabada:Qaado qiyaastii 3G budo ganaax ah oo alaabta ah, si sax ah u miisaam, ku rid weel koofiya leh oo qaboojiyaha, si sax ah ugu dar 50ml of methanol, miisaan, kulayl oo dib u soo celi 2 saacadood, qabooji, miisaan, samee miisaanka lumay. methanol, si fiican u rux oo sifee.Si sax ah u cabbir 25ml oo ah sifeeynta joogtada ah, ku rid weel, ka soo kabso dareeraha engegan, ku dar 10ml oo biyo ah si aad u milmaan inta soo hartay, ku dar biyo, ku rux n-butanol 5 jeer, 10ml mar kasta, isku dar n. -Xalka butanol, ku maydh laba jeer xal tijaabada ammonia, 5ml mar kasta, iska tuur xalalka ammonia, oo ka soo bax milixda n-butanol si ay u engegaan.Ku milmi hadhaagii 80% methanol kuna wareeji weel mug leh oo 50ml ah, ku dar 80% methanol miisaanka oo si fiican u rux.

Habka go'aaminta waxay si sax ah u cabbirtaa 2 ~ 5ml ee xalka tijaabada, ku rid 10ml tuubada tijaabada qalalan ee qalalan, sida waafaqsan habka hoos yimaada diyaarinta qalooca caadiga ah, cabbir nuugista sida waafaqsan sharciga "qallajinta dareeraha biyaha qubeyska", akhri cadadka ruscoegenin ee xalka tijaabada ka soo qalooca caadiga ah oo xisaabi.

Wadarta saponins ee Ophiopogon japonicus waa in aanay ka yarayn 0.12% oo ku salaysan Ruscogenin (C27H42O4).

Xaaladaha Chromatographic: (Tixraac kaliya)