Alaabta

Typhonine glycoside

Puhuang waa manka la qalajiyey ee Typha angustifolia L., Typha Orientalis Presl ama dhirta la midka ah ee Typhaceae.Waxa ku jira isorhamnetin-3-o-neohesperidin ee Pharmacopoeia Shiinaha (Daabacaada 2005) waxaa go'aamiya HPLC.Hababka ugu muhiimsan ee go'aaminta isorhamnetin-3-o-neohesperidin ee Typhae iyo diyaarinteeda waa

HPLCSida waafaqsan go'aaminta nuxurka Typha ee Pharmacopoeia Shiinaha (Daabacaada 2005):

Xaaladaha Chromatographic iyo Imtixaanka ku habboonaanta nidaamka: octadecyl silane bonded silica jel sida buuxin;Biyaha Acetonitrile (15:85) ayaa loo isticmaalay sidii wejiga wareega;Dhererka mowjadda ogaanshaha waxay ahayd 254 nm.Heerkulka tiirka 40 ℃.Tirada taarikada aragtida waa in aanay ka yarayn 1000 marka loo eego heerka ugu sarreeya ee isorhamnetin-3-o-neohesperidin.

Diyaarinta xalka tijaabada: qaado qiyaastii 0.5g oo badeecadan ah, si sax ah u miisaan, ku rid dhalada cabbirka 50ml, ku dar 45ml methanol, daawaynta ultrasonic (awood 250W, inta jeer ee 20KHz) 30 daqiiqo, qabooji, ku dar methanol miisaan, si fiican u rux, sifee, oo qaado sifaynta joogtada ah

Go'aaminta isorhamnetin-3-o-neohesperidin ee Puhuang iyo diyaarinteeda HPLC.Nidaamka wajiga wareega guud waa biyaha acetonitrile:

① Hp1050 chromatograph dareeraha waxqabadka sare;Tiirka Chromatographic C18 (5) μ m,4.6mm × 250mm) Dalian Institute of Chemical Physics, Akadeemiyada Sayniska Shiinaha);Marxaladda wareega: biyaha acetonitrile (15:85);Dhererka hirarka: 254nm;Heerkulka aqalka dhirta lagu koriyo ee tiirka.

② Waxqabadka sare ee chromatograph dareeraha ah waa nidaamka hp1050 ee Shirkadda Hewlett Packard ee Maraykanka;Tiirka Chromatographic bondclone 10 C18 (3.9mm) × 300mm)dhererka mowjadda ogaanshaha 254 nm, bandwidth 4 nm;hirarka dhererka dhererka 550 nm, bandwidth 100 nm;dhimis 8.

Fu Daxu iyo al.Lagu go'aamiyay waxa ku jira typhoniflorin iyo isorhamnetin-3-o-neohesperidin ee wadarta flavone-ka laga soosaaray Typha ee HPLC iyo 1100 chromatograph.Tiirka chromatographic waa madoobaad XdB C18 (250mm × 4.6mm, 5 μm) ； Wajiga wareega: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15: 78: 7; 15min: 23:70:7);Dhererka mowjadda ogaanshaha waa 248 nm.Diyaarinta wadarta flavonoids-ka laga soosaaray Typha: 50g oo Typha ah waxaa lagu soo saaray 60% ethanol (500ml) × 2), 1H mar kasta, soosaarku wuxuu ku ururay 40 ℃, soosaarku wuxuu ku milmay 600ml oo biyo ah iyo centrifused.Hadhaaga waxa lagu milmay 300ml oo biyo ah oo la badhxay.Isku darka xalka centrifugal, ku hagaaji mugga ilaa 1000ml biyo, ku dheji tiir of resin macroporous (150ml xabagta macroporous miisaanka qalalan 75g) 1.5 jeer qiyaasta daroogada cayriin, elute 600ml oo biyo ah iyo 450ml of 20% ethanol si meesha looga saaro. wasakhda, ka dibna lumin 600ml of 50% ethanol, iyo eluent waxaa lagu helay by faakuum qalajiyey at 40 ℃.

Yang Yonghua iyo al.Waxaa la adeegsaday koromografiga dareeraha waxqabadka sare iyo waxqabadka sare ee dareeraha si loo go'aamiyo waxa ku jira isorhamnetin 232o2, neohesperidin iyo typhoside ee Puhuang.Hpce-3d wax qabadka sare ee qalabka korantada korantada (Hewlett Packard, USA), tiirka quartz capillary 50 μm × 56cm (HP), lc-6a chromatograph dareeraha waxqabadka sare (shirkadda Shimadzu ee Japan), tiirka C18 5mm × 200mm, cabbirka qayb 5 μ M (Shirkadda Dalian Yite).Xaaladaha HPCE: buffer 0.02mol/l borax-0.05mol/l 10% acetonitrile of sodium dodecyl sulfate (SDS), danab 20kV, heerkulka tiirka 30 ℃, mowjadda ogaanshaha 270nm, mugga duritaanka 30KPa · s.Xaaladaha HPLC: Marxaladda guurguura waxay ahayd biyo acetonitrile (15:85), heerkulka tiirka wuxuu ahaa 25 ℃, iyo hirarka la ogaado wuxuu ahaa 254 nm.

Walaxda Tixraaca Typhaneoside

[name]typhonioside [1]

[CAS Maya.]104472-68-6

[habka ogaanshaha]HPLC ≥ 98%

[Tilmaamin]20mg 50mg 100mg 500mg 1g (waxaa lagu baakayn karaa si waafaqsan shuruudaha macaamiisha)

[hantida]budada amorphous